Prevalence of Hepatitis B Virus and Genotypes in the Region of Khyber Pakhtunkhwa Pakistan

GOHAR, M; REHMAN, IU; AHMAD, J; AHMAD, F; BASHIR, K; IKRAM, S; HASSAN, N; KHAN, MA; ULLAH, A

doi:10.54112/bbasr.v2023i1.53

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…Ciprofloxacin, a broad-spectrum fluoroquinolone antibiotic, was selected as the standard drug S* owing to its efficacy against both gram-positive and gram-negative bacteria (Suleman 2016 ; AMIN et al 2023 ; Hassan et al 2023 ). It may act by inhibiting the activity of crucial bacterial enzymes, namely topoisomerase IV and topoisomerase II (DNA gyrase), which play vital roles in the separation of bacterial DNA (Khan et al 2018 ; GOHAR et al 2023 ; Ullah et al 2023a , b ).…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, bacterial, viral, and other microbial infections can all cause respiratory tract infections. Specific bacterial pathogens, including Staphylococcus aureus , Streptococcus pneumonia , and hemophilic influenza , were commonly responsible for bacterial respiratory infections (Wu et al 2021 ; GOHAR et al 2023 ; Hassan et al 2023 ). Hemophilic influenza is not only a primary cause of pneumonia but can also lead to bronchitis and sinusitis (Diaz-Diaz et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial potential of Propolis: molecular docking, simulation and toxicity analysis

Islam,

Hussain,

Shujaat

et al. 2024

AMB Expr

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The issue of antibiotic resistance in pathogenic microbes is a global concern. This study was aimed to explore in silico and in vitro analysis of the antibacterial efficacy of different natural ligands against bacterial activity. The ligands included in the study were Propolis Neoflavanoide 1, Carvacrol, Cinnamaldehyde, Thymol, p-benzoquinone, and Ciprofloxacin (standard drug S*). The outcomes of molecular docking revealed that Propolis Neoflavaniode-1 showed a highly significant binding energy of − 7.1 and − 7.2 kcal/mol for the two gram-positive bacteria, as compared to the gram-negative bacteria. All ligands demonstrated acute toxicity (oral, dermal), except for Propolis Neoflavanoide 1 and S* drugs, with a confidence score range of 50–60%. Using a molecular dynamic simulation approach, we investigated Propolis Neoflavaniode-1’s potential for therapeutic use in more detail. An MD simulation lasting 100 ns was performed using the Desmond Simulation software to examine the conformational stability and steady state of Propolis Neoflavaniode-1 in protein molecule complexes. Additionally, in vitro studies confirmed the antimicrobial activity of Propolis Neoflavaniode 1 by increasing the zone of inhibition against Gram-positive bacteria, p < 0.005 as compared to gram-negative bacteria. This study revealed the promising antibacterial efficacy of Propolis Neoflavaniode 1, demonstrated through robust in silico analyses, minimal toxicity, and confirmed in vitro antimicrobial activity, suggesting its potential as a viable alternative to combat antibiotic resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial potential of Propolis: molecular docking, simulation and toxicity analysis

Islam,

Hussain,

Shujaat

et al. 2024

AMB Expr

View full text Add to dashboard Cite

show abstract

“…Subsequent research in children aged 6 to 11 revealed notable gains in lung function, but not in BMI or CFQ-R ratings; several unfavorable side-effects forced the trial’s termination. Although the effectiveness of the combination therapy is recognized, its usage is restricted due to concerns about drug interactions and safety [ 62 , 76 , 77 , 78 , 79 ].…”

Section: Monotherapy To Triple Combination Therapy (Eti)mentioning

confidence: 99%

Cystic Fibrosis: Understanding Cystic Fibrosis Transmembrane Regulator Mutation Classification and Modulator Therapies

Anwar,

Peng,

Zahid

et al. 2024

ARM

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A common life-threatening hereditary disease, Cystic Fibrosis (CF), affects primarily Caucasian infants. High sweat-salt levels are observed as a result of a single autosomal mutation in chromosome 7 that affects the critical function of the cystic fibrosis transmembrane regulator (CFTR). For establishing tailored treatment strategies, it is important to understand the broad range of CFTR mutations and their impacts on disease pathophysiology. This study thoroughly investigates the six main classes of classification of CFTR mutations based on their functional effects. Each class is distinguished by distinct molecular flaws, such as poor protein synthesis, misfolding, gating defects, conduction defects, and decreased CFTR expression at the apical membrane. Furthermore, this paper focuses on the emerging field of CFTR modulators, which intend to restore CFTR function or mitigate its consequences. These modulators, which are characterized by the mode of action and targeted mutation class, have the potential to provide personalized therapy regimens in CF patients. This review provides valuable insights into the genetic basis of CF pathology, and highlights the potential for precision medicine methods in CF therapy by thoroughly investigating CFTR mutation classification and related modulators.

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“…This genetic variation leads to substituting valine (Val) with methionine (Met) at codon 66 of the proBDNF protein. The Val66Met SNP has demonstrated effects on the intracellular transportation and activity-dependent release of BDNF demonstrated effects on the intracellular transportation and activity-dependent release of (Egan et al 2003 ; Dempster et al 2005 ; Szeszko et al 2005 ; PERVAIZ et al 2021 ; GOHAR et al 2023 ). The Val66Met SNP has a significant influence on BDNF secretion and function.…”

Section: Introductionmentioning

confidence: 99%

In silico analysis of the Val66Met mutation in BDNF protein: implications for psychological stress

Shan,

Khan,

Ishtiaq

et al. 2024

AMB Expr

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The brain-derived neurotrophic factor (BDNF) involves stress regulation and psychiatric disorders. The Val66Met polymorphism in the BDNF gene has been linked to altered protein function and susceptibility to stress-related conditions. This in silico analysis aimed to predict and analyze the consequences of the Val66Met mutation in the BDNF gene of stressed individuals. Computational techniques, including ab initio, comparative, and I-TASSER modeling, were used to evaluate the functional and stability effects of the Val66Met mutation in BDNF. The accuracy and reliability of the models were validated. Sequence alignment and secondary structure analysis compared amino acid residues and structural components. The phylogenetic analysis assessed the conservation of the mutation site. Functional and stability prediction analyses provided mixed results, suggesting potential effects on protein function and stability. Structural models revealed the importance of BDNF in key biological processes. Sequence alignment analysis showed the conservation of amino acid residues across species. Secondary structure analysis indicated minor differences between the wild-type and mutant forms. Phylogenetic analysis supported the evolutionary conservation of the mutation site. This computational study suggests that the Val66Met mutation in BDNF may have implications for protein stability, structural conformation, and function. Further experimental validation is needed to confirm these findings and elucidate the precise effects of this mutation on stress-related disorders.

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Prevalence of Hepatitis B Virus and Genotypes in the Region of Khyber Pakhtunkhwa Pakistan

Cited by 5 publications

References 28 publications

Antibacterial potential of Propolis: molecular docking, simulation and toxicity analysis

Antibacterial potential of Propolis: molecular docking, simulation and toxicity analysis

Cystic Fibrosis: Understanding Cystic Fibrosis Transmembrane Regulator Mutation Classification and Modulator Therapies

In silico analysis of the Val66Met mutation in BDNF protein: implications for psychological stress

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