Assessment of nutritional compounds and antinutritional factors in pea (<i>Pisum sativum</i>) seeds

Vidal-Valverde, Concepción; Frı́as, Juana; Hernández, Amelia; Martín-Álvarez, Pedro J.; Sierra, Isabel; Rodríguez, Carlos Lado; Blázquez, I.; Vicente, Gema

doi:10.1002/jsfa.1309

Cited by 93 publications

(67 citation statements)

References 43 publications

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“…It is also to be said that stachyose and verbascose are the most abundant oligosaccha- Table 3. Contents of phenolic acids (average ± standard deviation) in native (P) and treated (V-0; V-I; V-II) field pea samples rides in pea seeds as reported by Reddy et al (1980), Alonso et al (2001) and Vidal-Valverde et al (2003). Stachyose and verbascose were also represented at the highest amounts according to our results.…”

Section: Resultssupporting

confidence: 75%

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Reduction in the content of antinutritional substances in pea seeds (Pisum sativum L.) by different treatments

Dvořák¹,

Pechová²,

Pavlata³

et al. 2005

Czech J. Anim. Sci.

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ABSTRACT:The goal of the trial was to reduce the content of antinutritional substances in pea (Pisum sativum L.) seeds in order to enhance its use in livestock nutrition. A variety of field pea (Pisum sativum L.) with a high content of antinutritional substances and favourable production traits (Gotik) was chosen. Native and heat-treated pea seeds were used to collect representative samples (n = 6) for analytical purposes. The technology (V-0 technology, Czech patent No. 285745) was further modified by adjusting the reactor temperature, the duration of exposure to that temperature, and the duration of ageing of the material treated in this way (V-I and V-II technologies). The methodology of treatment is based on exposing pea seeds to vapour, organic acids and selected oxides. The monitored parameters included antinutritional substances. As far as the antinutritional substances were concerned, the content of trypsin inhibitors in native pea seeds (P) was around 15.4 ± 0.5 TIU. After treatment with technologies V-0, V-I, and V-II its activity dropped by 83.8, 80.5 and 83.8%, respectively. The pre-treatment titre of lectins (P) was 717 ± 376. It dropped by 70.3, 35.7 and 73.2% after treatment with technologies V-0, V-I and V-II, respectively. The content of tannins measured by the amount of gallic acid in native pea seeds was 49.1 ± 2.7 mg per kg. It dropped by 41.4, 32.0 and 46.2% after the application of the above-mentioned technologies. The content of indigestible oligosaccharides causing flatulence was less affected by the treatments. The pre-treatment content of raffinose was 9.5 ± 0.5 g/kg. The drop associated with the treatment was 9.5, 6.3 and 10.5%, respectively. The pretreatment content of stachyose was 21.4 ± 0.8 g/kg and after treatment with technologies V-0 and V-II it dropped by 7.0% and by 16.4%, respectively. The application of technology V-I did not result in a drop in the content of stachyose. The content of verbascose in native pea seeds was 16.1 g/kg and the treatment with technologies V-0; V-I and V-II resulted in a drop by 7.5, 5.6 and 20.5%, respectively. As for the detected phenolic acids, with the exception of caffeic acid, not a drop, but an increase in their content was recorded. Isoflavone oestrogens such as daidzein and genistein also recorded a small increase in their content. The results of the trial lead us to conclude that the above-described methods of pea seed treatment, especially the V-II variant, proved to be useful and can be recommended for practical use.

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Section: Resultssupporting

confidence: 75%

“…It is mainly stachyose and verbascose that are regarded as the key oligosaccharides in pea seeds (Reddy et al, 1980;Alonso et al, 2001;Vidal-Valverde et al, 2003). The above-mentioned sugars have no reducing effect and contain α-galactosidoglucosic and α-galactosidogalactosic bonds.…”

mentioning

confidence: 99%

Reduction in the content of antinutritional substances in pea seeds (Pisum sativum L.) by different treatments

Dvořák¹,

Pechová²,

Pavlata³

et al. 2005

Czech J. Anim. Sci.

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“…According to authors, a stachyose content was from 0.7 to 4.1 % of seed dry weight (g/100 g DW), but a verbascose content was from 3.1 % to a very low amount. Then Vidal-Valverde et al (2003) analyzed a variation in 18 pea accessions originating from a Valladolid (Spain) germplasm collection. They described the following results: total a-D-galactosides, 22.6-63.4 g/kg DW; stachyose, 10.7-26.7 g/kg DW; verbascose, 0.0-26.7 g/kg DW; raffinose, 4.1-10.3 g/ kg DW; and sucrose, 11.6-25.4 g/kg DW.…”

Section: Introductionmentioning

confidence: 99%

Raffinose family oligosaccharides in seeds of Pisum wild taxa, type lines for seed genes, domesticated and advanced breeding materials

Gawłowska

Świecicki

Lahuta

et al. 2016

Genet Resour Crop Evol

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The content of raffinose family oligosaccharides (RFOs) in pea seeds constrains their usage in feeding humans and animals. In our research, the content of soluble carbohydrates-particularly a-Dgalactosides of sucrose (RFOs)-was analyzed. The materials were as follows: 248 accessions from the Polish Pisum Genebank including representatives of taxa (from species to convarietas), type lines for genes controlling seed characters, and breeding materials and cultivars. Accessions were divided into homogeneous groups considering content of total soluble carbohydrates, total RFOs and individual RFOs: raffinose, stachyose and verbascose. The highest content of total soluble carbohydrates and total RFOs were stated for accessions with wrinkled seeds (r and rb genes) and the lowest content for seeds of the wild species P. fulvum Sibth. et Sm. Accessions valuable for breeding (for further decreasing of anti-nutritional compounds) were found among domesticated taxa (P. sativum subsp. sativum convar. vulgare Alef. and speciosum (Dierb.) Alef.), breeding lines, and some wild taxa. Accessions with decreased content of a total RFOs and verbascose are particularly valuable. It was found that the content of total RFOs was the most highly, frequently, and positively correlated with a stachyose and verbascose content. However, in P. fulvum seeds with the lowest content of RFOs and verbascose, a high correlation between the content of total RFOs and stachyose was revealed. Contents of all oligosaccharides were substantially lower in lines with dominant alleles of main pea seed genes (R, A, and I). It can be assumed that wild, primitive peas were characterized by not-all-tohigh (rather not high) content of oligosaccharides; then recessive mutations in mentioned genes resulted in an increased content of RFOs. It seems to be an interesting observation from an evolutionary point of view.

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“…As a rich source of proteins, carbohydrates, fibre, vitamins and minerals, peas are important in human nutrition [5]. Pea is the fourth leading legume in terms of consumption in the world after soybean, peanuts and bean [6]. One of the major abiotic stress often occurs in Europe is osmotic stresses induced with polyethylene glycol (within −0.5 MPa).…”

Section: Introductionmentioning

confidence: 99%

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

Brosowska-Arendt

Weidner

2012

Acta Soc Bot Pol

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IntroductionChanges in the environment, such as drought, salinity, high or low temperature, are an important factor which affects the growth of crops and the volume of crop yields [1][2][3]. Very important legume grown and consumed extensively worldwide is pea [4]. As a rich source of proteins, carbohydrates, fibre, vitamins and minerals, peas are important in human nutrition [5]. Pea is the fourth leading legume in terms of consumption in the world after soybean, peanuts and bean [6]. One of the major abiotic stress often occurs in Europe is osmotic stresses induced with polyethylene glycol (within −0.5 MPa). The earliest metabolic change caused by water stress is a decreased amount of polysomes [7]. Once the polysomes have been disaggregated, the plant growth is inhibited due to a slower protein synthesis rate [7][8][9]. Considerable reduction in the amount of polysomes in plant tissues is observable after osmotic stress lasting for just 20-30 minutes [10]. Decrease of the content of polysomes in response to abiotic stresses is connected with the process of "switching" the expression of genes from those participating in the growth and development of plants under unstressed conditions to the ones active in response to stress [11].In plant tissues, polysomes can occur as free polysomes (FP), endoplasmic reticulum membrane-bound polysomes (MBP) [12], cytoskeleton-bound polysomes (CBP) [13,14] and cytoskeleton-membrane-bound polysomes (CMBP) [15]. Changes in the distribution of polysomes between the particular fractions reflect changes in the complement of proteins, as each population of polysomes is engaged in the synthesis of specific proteins [16]. Thus, the FP population is mainly involved in the synthesis of soluble proteins of the cytoplasm, cellular nucleus, mitochondria and peroxisomes [17]; the MBP population is engaged in the production of secretory proteins, lysosome proteins and the proteins which are an integral component of the plasmatic membrane and intracellular membranes, including the endoplasmic reticulum [16,17]; finally, the CBP and CMBP populations are responsible for the synthesis of cytoskeleton and stress proteins [16,[18][19][20][21]. The largest share in the total polysome pool consists of the CBP population, which sometimes reaches 70% of the total polysome content [22]. Polysomes contain mRNAs, which may undergo selective translation, result in modification of protein synthesis in response to stress conditions in plants [23,24].The objective of this study was to indicate differences in the composition of polysome-bound proteins and in the products of in vitro translation in pea (Pisum sativum L.) seeds germinating under unstressed conditions and under long-and short-term osmotic stress (−0.5 MPa) followed by post-stress recovery. AbstractPlant growth throughout the world is often limited by unfavourable environmental conditions. This paper reports results of a study on long-and short-term osmotic stress (−0.5 MPa) followed by a recovery on in vitro translational capacity of polysomes a...

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Assessment of nutritional compounds and antinutritional factors in pea (Pisum sativum) seeds

Cited by 93 publications

References 43 publications

Reduction in the content of antinutritional substances in pea seeds (Pisum sativum L.) by different treatments

Reduction in the content of antinutritional substances in pea seeds (Pisum sativum L.) by different treatments

Raffinose family oligosaccharides in seeds of Pisum wild taxa, type lines for seed genes, domesticated and advanced breeding materials

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

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