Assessment of myoblast circular RNA dynamics and its correlation with miRNA during myogenic differentiation

Zhang, Pengpeng; Xu, Haixia; Rui, Li; Wu, Wei; Chao, Zhe; Li, Cencen; Xia, Wei; Wang, Lei; Yang, Jinzeng; Xu, Yongjie

doi:10.1016/j.biocel.2018.04.016

Cited by 22 publications

(25 citation statements)

References 39 publications

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“…The analysis of differentially expressed circRNAs during myogenic differentiation was also performed in cultured myogenic cells, both in C2C12 murine myoblasts and in primary human myoblasts [ 43 , 61 , 62 ] ( Table 1 ).…”

Section: Circular Rnas In Myogenesismentioning

confidence: 99%

“…In one study [ 62 ], deep RNA-seq of C2C12 myoblasts during cell differentiation revealed 37,751 unique circRNAs derived from 6943 hosting genes. A few randomly chosen circRNAs were validated by qPCR and RNA fluorescence in situ hybridization.…”

Section: Circular Rnas In Myogenesismentioning

confidence: 99%

“…Gene ontology of the host genes showed that many down-regulated circRNAs were related to the cell division and cell cycle, whereas up-regulated circRNAs were associated with the cell development process. Bioinformatics analysis of potential circRNA-miRNA interactions identified miRNAs involved in the regulation of myoblast differentiation, such as miR-133, miR-24, and miR-23 [ 62 ]. In another investigation [ 61 ], expression profiles of C2C12 myoblasts and myotubes were assessed using microarrays.…”

Section: Circular Rnas In Myogenesismentioning

confidence: 99%

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Circular RNAs in Muscle Function and Disease

Greco

Cardinali

Falcone

et al. 2018

IJMS

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Circular RNAs (circRNAs) are a class of RNA produced during pre-mRNA splicing that are emerging as new members of the gene regulatory network. In addition to being spliced in a linear fashion, exons of pre-mRNAs can be circularized by use of the 3′ acceptor splice site of upstream exons, leading to the formation of circular RNA species. In this way, genetic information can be re-organized, increasing gene expression potential. Expression of circRNAs is developmentally regulated, tissue and cell-type specific, and shared across eukaryotes. The importance of circRNAs in gene regulation is now beginning to be recognized and some putative functions have been assigned to them, such as the sequestration of microRNAs or proteins, the modulation of transcription, the interference with splicing, and translation of small proteins. In accordance with an important role in normal cell biology, circRNA deregulation has been reported to be associated with diseases. Recent evidence demonstrated that circRNAs are highly expressed in striated muscle tissue, both skeletal and cardiac, that is also one of the body tissue showing the highest levels of alternative splicing. Moreover, initial studies revealed altered circRNA expression in diseases involving striated muscle, suggesting important functions of these molecules in the pathogenetic mechanisms of both heart and skeletal muscle diseases. The recent findings in this field will be described and discussed.

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Section: Circular Rnas In Myogenesismentioning

confidence: 99%

Section: Circular Rnas In Myogenesismentioning

confidence: 99%

Section: Circular Rnas In Myogenesismentioning

confidence: 99%

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Circular RNAs in Muscle Function and Disease

Greco

Cardinali

Falcone

et al. 2018

IJMS

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“…Myogenesis is the process by which a somatic cell undergoes a series of proliferation, migration, and differentiation to form muscle tissue during development of the embryo [9,10]. Recent studies have shown that circular RNAs (circRNAs) have been identified as emerging non-coding RNAs during skeletal muscle development and play a crucial role in muscle development [11,12,13,14,15,16,17,18,19], but the specific functions in the proliferation and differentiation of myoblast cells are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation

Liu

et al. 2019

Cells

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CircRNA is a type of closed circular non-coding RNA formed by reverse splicing and plays an important role in regulating the growth and development of plants and animals. To investigate the function of circ-FoxO3 in mouse myoblast cells’ (C2C12) differentiation and proliferation, we used RT-qPCR to detect the expression level of circ-FoxO3 in mouse myoblast cells at different densities and different differentiation stages, and the specific interference fragment was used to inhibit the expression level of circ-FoxO3 in myoblast cells to observe its effect on myoblast cells proliferation and differentiation. We found that the expression level of circ-FoxO3 in myoblast cells increased with the prolongation of myoblast cells differentiation time, and its expression level decreased with the proliferation of myoblast cells. At the same time, we found that the differentiation ability of the cells was significantly increased (p < 0.05), but the cell proliferation was unchanged (p > 0.05) after inhibiting the expression of circ-FoxO3 in myoblast cells. Combining the results of bioinformatics analysis and the dual luciferase reporter experiment, we found that circ-FoxO3 is a sponge of miR-138-5p, which regulates muscle differentiation. Our study shows that circ-FoxO3 can inhibit the differentiation of C2C12 myoblast cells and lay a scientific foundation for further study of skeletal muscle development at circRNA levels.

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“…MiR is a class of evolutionarily conserved, regulatory noncoding RNAs of 18–24 nucleotides that participate in the fine-tuning of many, if not all, fundamental biological processes, including skeletal muscle development and muscle-related diseases [ 15 , 16 ]. MiRs have been exquisitely described to control gene expression by binding to mRNA targets using the seed sequences, and miRs with the same or similar seed sequences and origin are grouped into one family or cluster [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIK3R1 and Modulating the PI3K/AKT Signaling

Zhu

Zhang

et al. 2018

Genes

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The microRNA (miR)-17 family is widely expressed in mammalian tissues and play important roles in various physiological and pathological processes. Here, the functions of miR-106a-5p, a member of miR-17 family, were explored during myogenic differentiation in C2C12 cell line. First, miR-106a-5p was found to be relatively lower expressed in two-month skeletal muscle tissues and gradually decreased upon myogenic stimuli. Forced expression of miR-106a-5p significantly reduced the differentiation index, fusion index as well as the expression of myogenic markers (MyoD, MyoG, MyHC, Myomixer, Myomarker). Meanwhile, the levels of phosphorylated AKT were reduced by overexpression of miR-106a-5p, and administration of insulin-like growth factor 1 (IGF1), a booster of myogenic differentiation, could recover all the inhibitory effects above of miR-106a-5p. Furthermore, miR-106a-5p was elevated in aged muscles and dexamethasone (DEX)-treated myotubes, and up-regulation of miR-106a-5p significantly reduced the diameters of myotubes accompanied with increased levels of muscular atrophy genes and decreased PI3K/AKT activities. Finally, miR-106a-5p was demonstrated to directly bind to the 3’-UTR of PIK3R1, thus, repress the PI3K/AKT signaling.

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Assessment of myoblast circular RNA dynamics and its correlation with miRNA during myogenic differentiation

Cited by 22 publications

References 39 publications

Circular RNAs in Muscle Function and Disease

Circular RNAs in Muscle Function and Disease

Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation

MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIK3R1 and Modulating the PI3K/AKT Signaling

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