Assessment of myeloperoxidase activity in whole rat kidney

Hillegass, Leonard M.; Griswold, D. E.; Brickson, B; Albrightson-Winslow, C R

doi:10.1016/0160-5402(90)90013-b

Cited by 462 publications

(292 citation statements)

References 13 publications

Supporting

Mentioning

282

Contrasting

Unclassified

Order By: Relevance

“…The MPO activity of PMNLs isolated from blood samples taken before and after treatment with iPPVO was measured in a procedure similar to that documented by Hillegas et al (1990). The protein content of the homogenate was measured with Lowry's method (Lowry et al 1951).…”

Section: Methodsmentioning

confidence: 99%

Effects of inactivated Parapoxvirus ovis on polymorphonuclear leukocyte function and myeloperoxidase activity in horses

Ülgen¹,

Yarami̇s²,

Rayaman³

et al. 2014

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:Immunomodulatory products have been used for years in veterinary medicine. Inactivated Parapoxvirus ovis (iPPVO) is currently used in equine medicine as an immunomodulator to improve the immune system and as a prophylactic treatment to prevent or treat infectious diseases. This study was designed to determine the effects of iPPVO on polymorphonuclear leukocyte (PMNL) function (phagocytosis and intracellular killing activity) and the myeloperoxidase (MPO) activity of PMNLs in horses. Twenty-four healthy English thoroughbred horses with an average age of 11 years were included in the study. Venous blood samples (10 ml) were taken before (agentfree controls) and after the administration of iPPVO (2 ml i.m. injection on Days 1, 3, and 5). PMNLs (1 × 10 7 cells/ml) were isolated from venous blood containing EDTA (0.1 g/ml) with Ficoll-Hypaque gradient centrifugation. Cellular phagocytosis and intracellular killing activities were assayed using a modification of Alexander's method before and after treatment with iPPVO. MPO activity was also measured. The administration of iPPVO significantly increased the phagocytic, intracellular killing, and MPO activities of equine PMNLs (P = 0.0058, P = 0.0050, and P = 0.0070, respectively). This study demonstrates a strong correlation between MPO activity and PMNL function. The administration of iPPVO to horses has a supportive effect on their cellular immunity and an immunomodulatory effect against equine viral infections.

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of inactivated Parapoxvirus ovis on polymorphonuclear leukocyte function and myeloperoxidase activity in horses

Ülgen¹,

Yarami̇s²,

Rayaman³

et al. 2014

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…Tissue-associated myeloperoxidase (MPO) activity was measured according to the procedure reported by Hillegas et al 13 . Hepatic tissue samples were homogenized in 50 mm potassium phosphate buffer (PB, pH 6.0) and homogenates were centrifuged at 41 400g for 10 min; pellets were suspended in 50 mm PB containing 0.5% hexadecyltrimethylammonium bromide.…”

Section: Laboratory Examinationsmentioning

confidence: 99%

Grape seed protects cholestatic rats liver from ischemia/reperfusion injury

et al. 2016

View full text Add to dashboard Cite

PURPOSE:To determine the effect of grape-seed extract against ischemia/reperfusion injury in cholestatic liver. METHODS:Eighteen Wistar albino rats were divided into three groups. In control and study groups, cholestasis was provided by bile duct ligation. Seven days later, the rats were subjected to 30 min hepatic ischemia, followed by 60 min of reperfusion. Oral administration of 50 mg/kg/day grape-seed extract was started 15 days before bile duct ligation and continued to the second operation in the study group. Serum, plasma and liver samples were taken. Laboratory analysis, tissue gluthation, malondialdehyde, myeloperoxidase levels and histopathological examination were performed.RESULTS: Significant decrease in liver gluthation level and significant increase in malondialdehyde level and myeloperoxidase activity were observed after ischemia/reperfusion in cholestatic rats. Serum and plasma levels for laboratory analysis were also significantly higher in cholestatic I/R group. Hepatic necrosis and fibrosis were detected in histopathological examination. Oral grape-seed extract administiration reversed all these parameters and histopathological findings except serum bilirubin levels. CONCLUSION:Oral grape-seed extract treatment can improve liver functions and attenuate the inflammation and oxidative stress in cholestatic ischemia/reperfusion injury.

show abstract

“…One unit of enzyme activity was defined as the amount of MPO present that caused a change in absorbance measured at 460 nm for 3 min. MPO activity was expressed as U/g tissue (29).…”

Section: Measurement Of Brain Myeloperoxidase (Mpo) Activitymentioning

confidence: 99%

The protective effect of Nigella sativa oil in the brain of the biliary obstructed rats

Toklu¹

2013

mpj

View full text Add to dashboard Cite

Oxidative stress is one of the important mechanisms of jaundice induced encephalopathy. The aim of this study was to examine the possible protective effect of Nigella sativa (NS) seed oil against the oxidative stress of brain tissue induced by experimental obstructive jaundice in rats.BiliarY obstruction was performed in male Wistar albino rats by bile duct ligation and scission (BDL). Intragastric NS oil (1 mg/kg p.o.) or saline was administered for 28 days. At the end of the experiment, in the half of the rats the blood brain barrier (BBB) permeability was evaluated by Evans blue (EB) extravasation. Other rats were decapitated and brain tissue samples were obtained for the measurement of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) and Na + ,K + -ATPase activities.ChronIC biliary obstruction caused a significant increase in the BBB permeability which was verified by EB extravasation while this effect was attenuated by NS oil treatment. On the other hand, BDL-induced decrease in brain GSH level and Na + ,K + -ATPase activity were elevated back to control level in NS oil-treated BDL group. Increase in tissue MDA level, and MPO activity due to BDL were also attenuated by NS oil treatment.Our results suggest that NS oil treatment protects the brain from oxidative damage following bile duct ligation in rats. This effect possibly involves the inhibition of neutrophil infiltration and lipid peroxidation and the restoration of antioxidant status in the tissue. Accordingly, supplementing cirrhotic patients with adjuvant therapy of NS oil may have some benefit against hepatic encephalopathy.

show abstract

Assessment of myeloperoxidase activity in whole rat kidney

Cited by 462 publications

References 13 publications

Effects of inactivated Parapoxvirus ovis on polymorphonuclear leukocyte function and myeloperoxidase activity in horses

Effects of inactivated Parapoxvirus ovis on polymorphonuclear leukocyte function and myeloperoxidase activity in horses

Grape seed protects cholestatic rats liver from ischemia/reperfusion injury

The protective effect of Nigella sativa oil in the brain of the biliary obstructed rats

Contact Info

Product

Resources

About