In this study, the scions of the Thompson Seedless grape cultivar were grafted onto three local grape cultivars (Chav Ga, Rash Miri and Tre Rash) rootstocks by wedge grafting in 2021-2022. The grafting were made manually on the February 15th by using one-year-old cuttings ( scion and rootstocks). Growth parameters such as shoot length and diameter, dry weight of vegetative growth, number of leaves and leaves dry weight showed significant differences while chlorophyll content showed non-significant differences among the cultivars.Generally, Thompson Seedless grafted on Rash Miri rootstock gave better results for most of the growth parameters in both seasons, while the grade of callus, rate of graft take, root percentage and root numbers showed significant differences among the cultivars in both two seasons. However, root length showed non-significant difference between all cultivars, moreover in Chav Ga cultivar showed a significant difference in root shoot ratio compared with the other two cultivars. Grade of callus was higher (1.95 and 1.99) in Rash Miri and lower was (1.75 and 1.78) in Chav Ga, for seasons 1 and 2,. Rate of graft take was also higher when Thompson Seedless grafted onto Tre Rash cultivar (88.13 and 89.00 %) and (74.52 and 75.80 %) in Chav Ga rootstock for two experimental seasons, respectively.