Assessment of microbial diversity in the river trout Salmo trutta fario L. intestinal tract identified by partial 16S rRNA gene sequence analysis

Abstract

The microbial diversity of the intestinal tract content of the river trout from two Lithuanian rivers has been investigated by molecular methods: polymerase chain reaction amplification and sequencing of partial 16S rRNA genes. Predominant bacterial populations detected in the river trout intestinal tract from the Skroblus River were Rahnella (21%), from the Xeimena River, Aeromonas (41.7%) and Plesiomonas (22.9%). Buttiauxella agrestis, Budvicia aquatica, Erwinia persicinus, Yersinia mollaretii, Y. kristensenii, Y. rohdei, Moellerella wisconsensis, Obesumbacterium proteus, Pantoea cedenensis, Rahnella aquatilis, and Rahnella sp. from the Enterobacteriaceae family have been detected in the intestinal tract of freshwater salmonid fish for the first time.KEY WORDS: 16S rRNA gene sequencing, intestinal microbiota, river trout.

Inhibition of fish pathogens by the microbiota from rainbow trout ( Oncorhynchus mykiss , Walbaum) and rearing environment

“…With regard to the taxonomic identification of the TM and LAB, our results (Table 1) point to the instability of the composition of the rainbow trout intestine microbiota along the fish life-cycle, which has been previously described for other salmonids [24]. With regard to this, at the larval stage, the predominant intestinal TM belonged to the family Aeromonadaceae, and mainly to the genus Aeromonas, which is in agreement with a previous study on river trout (Salmo trutta) [25]. On the other hand, in our study, the intestinal microbiota found from the growth stages F1 to A2 was dominated by Enterobacteriaceae (mainly the genera Citrobacter, Kluyvera and Enterobacter), similarly as previously described [26].…”

Erwinia typographi sp. nov., isolated from bark beetle (Ips typographus) gut

“…Genomic DNA was extracted from the isolates following the method reported by Skrodenyte-Arbaciauskiene et al (2006b). Universal bacterial primers w001 F8 (59-AGAGTTTGATCMTGGCTC-39) and w002 R1509 (59-GNTACCTTGTTACGACTT-39) (Godon et al, 1997) were used to amplify 16S rRNA gene sequences.…”

Changes in microbiota of rainbow trout caused by sediments contamination

“…The digestive endosystem contains micro-organisms which use for their nutrition the substances isolated by other microorganisms during metabolism [24][25][26][27][28][29][30][31].…”