Assessment of low amounts of meat and bone meal in the diet of laying hens by using stable isotopes

Madeira, Luciene Aparecida; Denadai, Juliana Célia; Ducatti, Carlos; Pezzato, Antônio Celso; Araújo, Priscila Cavalca de; Sartori, Maria Márcia Pereira; Pizzolante, Carla Cachoni; Sartori, José Roberto

doi:10.5433/1679-0359.2015v36n2p1155

Cited by 3 publications

(3 citation statements)

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“…Similarly fast isotopic changes in rapidly turning over body pools have been documented in mammals and birds (Ayliffe et al ; Podlesak et al ; Sponheimer et al ). Both the egg albumen values and the offsets between egg albumen and diet in both carbon and nitrogen isotopic values show some degree of variability (Table ), but are similar to those found in large‐scale studies of eggs and egg‐laying hens in the Netherlands, New Zealand and Brazil (Madeira et al ; Rogers et al ).…”

Section: Discussionsupporting

confidence: 74%

Chicken and Egg: Testing the Carbon Isotopic Effects of Carnivory and Herbivory

O’Connell

Hedges

2016

Archaeometry

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13C bioapatite-diet spacing, and not that of Δ 13C collagen-diet . KEYWORDS: BIOMINERAL, ISOTOPE ANALYSIS, PALAEODIET, ISOTOPE ECOLOGY INTRODUCTIONThe relationship between the carbon and nitrogen isotope composition of an individual's diet and their body tissues is now frequently employed as a method of dietary analysis in several different research fields, including ecology, archaeology and physiology (Gannes et al. 1998; Lee-Thorp 2008;Boecklen et al. 2011). The underlying basis of such work is that there is an isotopic 'fine structure' within the biosphere due to fractionation during natural processes, which results in isotopic differences between food types. An individual's diet can thus be inferred from the isotopic signature, which is transferred to and retained in the body during the absorption and incorporation of food.The interpretation of diet is predicated on understanding and therefore accounting for any isotopic fractionation resulting from the incorporation of diet into body tissues. Over the decades, there have been a number of empirical studies aimed at elucidating isotopic fractionation during the body's metabolism, yet some observed patterns remain puzzling. One such is the offset between the carbon isotopic values of the biomineral and protein components of bone. This study *Received 21 January 2016; accepted 24 March 2016 †Corresponding author: email tco21@cam.ac.uk Archaeometry 59, 2 (2017Archaeometry 59, 2 ( ) 302-315 doi: 10.1111 This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Krueger and Sullivan (1984) found a Δ 13 C ap-coll spacing of +7‰ for modern herbivores (n = 20), and +3-4‰ for modern carnivores (n = 40). Their analyses of over 200 archaeological humans showed that the majority had Δ 13 C ap-coll spacings of +3 to +7‰, as would be predicted for omnivores. In modern southern African wild fauna, Lee-Thorp et al. (1989) found that the Δ 13 C ap-coll was +6.8 ± 1.4‰ for herbivores (n = 67) and +4.3 ± 1.0‰ for carnivores (n = 49), with both groups having similar bioapatite δ C ap-coll between herbivores (+7.6 ± 0.5‰, n = 46) and carnivores (+4.8 ± 0.4‰, n = 27). Similar Δ 13 C ap-coll values have been found in a range of other ungulate herbivores (Schoeninger and DeNiro 1982;Nelson et al. 1986;Kellner and Schoeninger 2007). In predominately herbivorous primates, Crowley et al. (2010) observed a mean Δ 13C ap-coll of +5.6‰, with some variability (range of +3.6 to +8.6‰). Ambrose (1993) analysed East African historical human bone, and showed that the Δ 13C ap-coll of pastoralists (Kalenjin, Turkana, Pokot and Dasenech) was +3.8 ± 1.2‰ (n = 8), whereas the Δ 13 C ap-coll of Kikuyu farmers was +5.5 ± 0.5‰ (n = 16), supporting the theory that those humans with a diet high in animal protein have a smaller spacing between their collagen and bioapatite δ 13 C than predominantly plant-eating farmers. Although based on a limited number of...

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Section: Discussionsupporting

confidence: 74%

Chicken and Egg: Testing the Carbon Isotopic Effects of Carnivory and Herbivory

O’Connell

Hedges

2016

Archaeometry

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“…The range of isotopic values found in serum associated with each diet needs to be defined to allow for effective traceability and the detection of ABPs (Mori et al, 2013;Sernagiotto et al, 2013;Madeira et al, 2014). The δ 13 C and δ 15 N values did not distinguish the experimental groups during the initial period of this study, showing the isotopic similarity of the groups when subjected to the same diet.…”

Section: Discussionmentioning

confidence: 81%

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

Silva

Biscola

Santos

et al. 2016

The Veterinary Journal

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Sheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie - a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for C andN to trace animal proteins in the serum of three groups of sheep: 1 - received only vegetable protein (VP) for 89 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16-89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed N equilibrium 5 days after MBM removal (54th day). Conversely,N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on naturalN signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δC and δN values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments.

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“…In the present study, only corn, soybean meal and increasing levels of BMBM were used as the major ingredients of the diets to avoid greater interference caused by the use of a large number of ingredients. Therefore, there is a need to verify whether these other ingredients could interfere in the detection of BMBM, because Madeira et al (2015) studied the same ingredients but their lower inclusion and low inclusion were not differentiated from BMBM. Thus, the present study aimed to verify whether the inclusion of other ingredients, such as wheat bran, corn gluten and yeast in the diets of laying hens affects the traceability of bovine meat and bone meal in eggs using stable carbon and nitrogen isotope analysis.…”

Section: Introductionmentioning

confidence: 99%