2013
DOI: 10.1016/j.enzmictec.2013.05.008
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Assessment of long-term cryopreservation for production of hCTLA4Ig in transgenic rice cell suspension cultures

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Cited by 8 publications
(4 citation statements)
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“…목적단백질 생산유도 4일 차 이후에는 세포 내부의 hCTLA4Ig의 양이 점차 감소하였다. 이는 세포의 생존도 감소와 세포 용해로 인해 세포 내부의 hCTLA4Ig가 용출되었기 때문이다 [21]. 하지만 세포가 용해 되면 세포내의 프로테아제가 배지내로 용출되어 목적단백질 의 분해가 일어나기 때문에 적절한 시기에 목적단백질이 분 비되는 것이 단백질의 품질에 있어 매우 중요하다.…”
Section: Resultsunclassified
“…목적단백질 생산유도 4일 차 이후에는 세포 내부의 hCTLA4Ig의 양이 점차 감소하였다. 이는 세포의 생존도 감소와 세포 용해로 인해 세포 내부의 hCTLA4Ig가 용출되었기 때문이다 [21]. 하지만 세포가 용해 되면 세포내의 프로테아제가 배지내로 용출되어 목적단백질 의 분해가 일어나기 때문에 적절한 시기에 목적단백질이 분 비되는 것이 단백질의 품질에 있어 매우 중요하다.…”
Section: Resultsunclassified
“…Furthermore, when transgenic cell lines are cultivated and undergo periodic subculturing for extended periods, the production levels of heterologous proteins may decrease or even cease completely because of gene silencing or recombination events. To cope with this issue, the generation of cell banks is a viable approach 43 , 44 , 45 . Therefore, although no notable changes on the expression of VP2 have been observed during the long-term propagation of the SMC-1 line, the establishment of cryopreservation approaches for carrot cell cultures could allow the generation of a cell bank to ensure productivity in the long term.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, Protalix's process provides an additional reference point to justify the feasibility of the process described in this Total Equipment Costs ($ million) Seed Train Production Reactor Extraction Clarification UF/DF DEAE Hupresin UF/DF 2 Unlisted F I G U R E 6 Total equipment costs for a greenfield single-product facility producing rrBChE in two-stage semicontinuous and two-stage batch mode operation broken down by manufacturing section. DEAE, Diethylethanolamine; rrBChE, rice recombinant butyrylcholinesterase; UF/DF, ultrafiltration/diafiltration [Color figure can be viewed at wileyonlinelibrary.com] (Kwon et al, 2013;Mustafa, de Winter, van Iren, & Verpoorte, 2011), but there is no protocol that can be universally applied to all species (Santos, Abranches, Fischer, Sack, & Holland, 2016). There is also an ongoing literature debate as to the potential immunogenicity of plant glycan structures.…”
Section: Alternate Case 1: New Facilitymentioning
confidence: 99%