Assessment of intermittent exposure of zinc oxide nanoparticle (ZNP)–mediated toxicity and biochemical alterations in the splenocytes of male Wistar rat

Singh, Neelu; Das, Monoj Kumar; Gautam, Rahul; Ramteke, Anand; Rajamani, Paulraj

doi:10.1007/s11356-019-06225-4

Cited by 13 publications

(12 citation statements)

References 38 publications

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“…Zinc is an antagonist of redox transition metals such as copper or iron [ 132 ]. On the other hand, excessive concentrations of selenium and zinc have cytotoxic effects and serious consequences of organism poisoning [ 129 , 133 , 134 ]. Secondly, they can act as exogenous free radical scavengers that protect DNA molecules from oxidative damage [ 128 , 135 ] ( Table 1 ).…”

Section: Pharmacological Interventions Protecting Genomementioning

confidence: 99%

Genome-Protecting Compounds as Potential Geroprotectors

Proshkina

Shaposhnikov

Moskalev

2020

IJMS

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Throughout life, organisms are exposed to various exogenous and endogenous factors that cause DNA damages and somatic mutations provoking genomic instability. At a young age, compensatory mechanisms of genome protection are activated to prevent phenotypic and functional changes. However, the increasing stress and age-related deterioration in the functioning of these mechanisms result in damage accumulation, overcoming the functional threshold. This leads to aging and the development of age-related diseases. There are several ways to counteract these changes: (1) prevention of DNA damage through stimulation of antioxidant and detoxification systems, as well as transition metal chelation; (2) regulation of DNA methylation, chromatin structure, non-coding RNA activity and prevention of nuclear architecture alterations; (3) improving DNA damage response and repair; (4) selective removal of damaged non-functional and senescent cells. In the article, we have reviewed data about the effects of various trace elements, vitamins, polyphenols, terpenes, and other phytochemicals, as well as a number of synthetic pharmacological substances in these ways. Most of the compounds demonstrate the geroprotective potential and increase the lifespan in model organisms. However, their genome-protecting effects are non-selective and often are conditioned by hormesis. Consequently, the development of selective drugs targeting genome protection is an advanced direction.

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Section: Pharmacological Interventions Protecting Genomementioning

confidence: 99%

Genome-Protecting Compounds as Potential Geroprotectors

Proshkina

Shaposhnikov

Moskalev

2020

IJMS

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“…Despite the benefits of ZnONPs in a variety of applications, many studies have shown potential health hazards of exposure to ZnONPs, including airway inflammation, spleen toxicity, neurotoxicity, etc. [2][3][4][5]. We reported previously, in mice, that a single high dose oropharyngeal aspiration for 2 days, or a repeated low dose aspiration of ZnONPs for 4 weeks, increases the total cell numbers in the bronchoalveolar lavage fluid (BALF) and pulmonary inflammation [6].…”

Section: Introductionmentioning

confidence: 99%

Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration

Chen

et al. 2020

IJMS

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Zinc oxide nanoparticles (ZnONPs) are frequently encountered nanomaterials in our daily lives. Despite the benefits of ZnONPs in a variety of applications, many studies have shown potential health hazards of exposure to ZnONPs. We have shown that oropharyngeal aspiration of ZnONPs in mice increases lung inflammation. However, the detailed mechanisms underlying pulmonary inflammatory cell infiltration remain to be elucidated. Endothelium functions as a barrier between the blood stream and the blood vessel wall. Endothelial barrier dysfunction may increase infiltration of immune cells into the vessel wall and underlying tissues. This current study examined the effects of ZnONPs exposure on endothelial barriers. ZnONPs exposure increased leukocyte infiltration in the mouse lungs. In endothelial cells, ZnONPs reduced the continuity of tight junction proteins claudin-5 and zonula occludens-1 (ZO-1) at the cell junctions. ZnONPs induced adherens junction protein VE-cadherin internalization from membrane to cytosol and dissociation with β-catenin, leading to reduced and diffused staining of VE-cadherin and β-catenin at cell junctions. Our results demonstrated that ZnONPs disrupted both tight and adherens junctions, compromising the integrity and stability of the junction network, leading to inflammatory cell infiltration. Thus, ZnONPs exposure in many different settings should be carefully evaluated for vascular effects and subsequent health impacts.

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“…Almansour (Almansour et al 2017 ) reported that the exposure of male rats to ZnO NPs (35 nm) for 21 days caused the dysfunction liver and kidney tissues and ZnO NPs induced inflammatory cell infiltration, sinusoidal dilatation, Kupffer cells hyperplasia, necrosis, hepatocytes hydropic degeneration, nuclear alterations, and hepatocytes glycogen depletion. Singh (Singh et al 2019 ) also reported the oral exposure of rats to a high dose (250 mg kg −1 ) of ZnO NPs (30 nm) for 28 days might cause the apoptotic death of their splenocytes, indicating that ZnO NPs induced tissue damage via oxidative stress by increasing the generation of ROS, thereby compromising the integrity of cellular membranes.…”

Section: Results and Disscusionmentioning

confidence: 99%

“…Thus, in this study were selected these nanomaterials to create core/shell NPs to modify and cause changes in their characteristics and reduce toxicity. Studies have also reported the toxicity of MgO (Mangalampalli et al 2017 ; Mangalampalli et al 2018 ) and ZnO NPs (Almansour et al 2017 ; Singh et al 2019 ). To the best of our knowledge and according to a literature survey, no report is available on the acute lethal effect and toxicity of biologic MgO/ZnO CS NPs.…”

Section: Introductionmentioning

confidence: 99%

Estimation of in vivo toxicity of MgO/ZnO core/shell nanoparticles synthesized by eco-friendly non-thermal plasma technology

2022

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MgO/ZnO core/shell nanoparticles were synthesized using the atmosphere plasma jets technique. The physical properties of the synthesized nanoparticles were investigated by a series of techniques, including X-ray diffraction (XRD), X-ray dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). XRD and EDS analyses confirmed the purity of the nanoparticles synthesized with an average nanoparticle crystallite size of 36 nm. TEM confirmed the successful synthesis of spindle-shaped MgO/ZnO core/shell nanoparticles with an average size of 70 nm. To evaluate their toxicity, the MgO/ZnO core/shell nanoparticles were tested in vivo. Twenty-five albino female rats were randomly divided into five groups (five rats in each group); one was used as the control group and the other four as the experimental groups. Doses of the MgO/ZnO core/shell nanoparticles solution were orally administered to the test groups to examine the toxicity. For 30 consecutive days, each rat in test groups 2–5 received 1 mL of the MgO/ZnO core/shell nanoparticles solution at the respective doses of 1.25, 2.5, 5, and 10 mg L −1 . The rats’ growth, hematology, thyroid gland function, and histopathology were examined after 30 days. Findings indicate that the growth retardation in the rats treated with MgO/ZnO core/shell nanoparticles may be due to their infection by Hyperthyroidism . The hematology results show the nonsignificant effect of MgO/ZnO core/shell nanoparticles on white blood cells, implying that these nanoparticles have no harmful impact on the immune system. Moreover, the levels of the thyroxine and thyroid‐stimulating hormones increased, and that of the triiodothyronine hormone decreased. The histological analysis results show that low concentrations of MgO/ZnO core/shell nanoparticles are safe for desired biomedical applications.

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Assessment of intermittent exposure of zinc oxide nanoparticle (ZNP)–mediated toxicity and biochemical alterations in the splenocytes of male Wistar rat

Cited by 13 publications

References 38 publications

Genome-Protecting Compounds as Potential Geroprotectors

Genome-Protecting Compounds as Potential Geroprotectors

Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration

Estimation of in vivo toxicity of MgO/ZnO core/shell nanoparticles synthesized by eco-friendly non-thermal plasma technology

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