Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls

Gillan, L.; Kroetsch, Tom; Maxwell, W.M.C.; Evans, G.

doi:10.1016/j.anireprosci.2006.12.010

Cited by 144 publications

(102 citation statements)

References 34 publications

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“…Mean VCL, VAP and ALH values were higher in high fertile bulls, whereas the opposite was found for LIN, STR, and BCF. Similar results have been reported in cattle bulls (Gillan et al, 2008) and in buffaloes (Kumar et al, 2014). Sperm kinematic analyses using CASA have shown variable correlations between particular motility patterns and field fertility in cattle bulls (Bailey et al, 1994;Holt et al, 1997;Zhang et al, 1998;Hirai et al, 2001;Januskauskas et al, 2003;Broekhuijse et al, 2012).…”

Section: Resultssupporting

confidence: 80%

Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility

Singh

Kumaresan

et al. 2016

IJAR

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The present study was undertaken to identify the differences in sperm kinematics between buffalo bulls with different fertility ratings. Murrah buffalo bulls (n=9) that were routinely used for breeding purpose under progeny testing programme were utilized for the study. Bull fertility was determined based on in vivo fertility trials and the conception rates (CR) were adjusted for different non-genetic parameters. Based on the adjusted CR, bulls were classified into high, medium and low fertile group. Frozen semen samples of these bulls were obtained and sperm kinematic parameters were assessed using a computer assisted sperm analyzer. The kinematic parameters analyzed included the curvilinear velocity (VCL), the linear velocity (VSL), the average path velocity (VAP), the amplitude of lateral head displacement (ALH), the linearity (LIN), the straightness coefficient (STR) and the beat cross frequency (BCF). In high fertile bulls, the proportion of motile spermatozoa was higher (p<0.001) than the medium and low fertile bulls. The VAP and VCL of sperm motion were significantly higher (P<0.05) in high fertile bulls compared to either medium or low fertile bulls. The VSL was significantly lower in low fertile bulls (P<0.005) compared to either high or medium fertile bulls. Spermatozoa from high fertile bulls had significantly higher (P<0.05) BCF, STR, ALH and LIN compared to either medium or low fertile bulls. Buffalo bull fertility was significantly and positively correlated with sperm motility, VAP, VSL, VCL and ALH.

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Section: Resultssupporting

confidence: 80%

Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility

Singh

Kumaresan

et al. 2016

IJAR

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“…In cattle, several sperm velocity parameters have been shown to be positively associated with fertility rates (Cseh et al 2004, Gillan et al 2008. In the present research, FSF spermatozoa frozen with the DF method displayed higher velocities (VAP, VSL, VCL) at the majority of time points during the 24-h post-thaw incubation than Control spermatozoa frozen with the straw or DF method.…”

Section: Discussionsupporting

confidence: 52%

Evaluation of motility, membrane status and DNA integrity of frozen–thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation

Montano¹,

Kraemer²,

Love³

et al. 2012

Reproduction

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Artificial insemination (AI) with sex-sorted frozen-thawed spermatozoa has led to enhanced management of ex situ bottlenose dolphin populations. Extended distance of animals from the sorting facility can be overcome by the use of frozen-thawed, sorted and recryopreserved spermatozoa. Although one bottlenose dolphin calf had been born using sexed frozen-thawed spermatozoa derived from frozen semen, a critical evaluation of in vitro sperm quality is needed to justify the routine use of such samples in AI programs. Sperm motility parameters and plasma membrane integrity were influenced by stage of the sex-sorting process, sperm type (non-sorted and sorted) and freezing method (straw and directional) (P!0.05). After recryopreservation, sorted spermatozoa frozen with the directional freezing method maintained higher (P!0.05) motility parameters over a 24-h incubation period compared to spermatozoa frozen using straws. Quality of sperm DNA of non-sorted spermatozoa, as assessed by the sperm chromatin structure assay (SCSA), was high and remained unchanged throughout freeze-thawing and incubation processes. Though a possible interaction between Hoechst 33342 and the SCSA-derived acridine orange was observed in stained and sorted samples, the proportion of sex-sorted, recryopreserved spermatozoa exhibiting denatured DNA was low (6.6G4.1%) at 6 h after the second thawing step and remained unchanged (PO0.05) at 24 h. The viability of sorted spermatozoa was higher (P!0.05) than that of non-sorted spermatozoa across all time points after recryopreservation. Collective results indicate that bottlenose dolphin spermatozoa undergoing cryopreservation, sorting and recryopreservation are of adequate quality for use in AI.

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“…These parameters mostly detect cases of significantly inadequate reproduction or infertility in bulls (Rodriguez-Martinez 1998). To assess the resistance and the fertilization ability of sperm have been developed biological tests of ejaculate, e.g., long-term cold test and short-term heat test of sperm survival, resistance of sperm against cold shock and determination the percentage of live and dead sperm by staining (Januskauskas et al 2000, Gillan et al 2008. Diluting of sperm, filling of straws, their cooling and freezing follow the macro-and micro-evaluation of ejaculate quality.…”

Section: Introductionmentioning

confidence: 99%

Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

et al. 2012

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The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk in extenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawed artificial insemination (AI) doses, and during thermodynamic testing compared to extenders not containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each of the 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMed and Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A total of 640 AI doses were analysed. The volume of samples, sperm concentration, and percentage of motile spermatozoa were evaluated after collection, as was sperm motility after thawing of AI doses and during thermodynamic testing. Percentages of live and dead sperm were also evaluated. The data set was analysed using SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA). The results confirmed significant (P<0.05-0.01) between-sire differences in the volume, density, and activity of sperm as well as in share of live and dead sperm after collection; in decline of sperm motility in the fresh ejaculate, after thawing, and during the entire thermodynamic test, as well as in the share of live and dead sperm after thawing. The extenders ranked by sperm motility are: Optidyl, Triladyl, AndroMed, and Bioxcell, demonstrating the higher quality of AI doses produced using egg yolk extenders. Differences in sperm motility were significant (P<0.05-0.01) during the entirety of thermodynamic testing. Egg yolk extenders had a significantly (P<0.05-0.01) higher percentages of live sperm after thawing.

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Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls

Cited by 144 publications

References 34 publications

Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility

Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility

Evaluation of motility, membrane status and DNA integrity of frozen–thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation

Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

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