Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

Beran, Jan; Stádník, Luděk; Bezdíček, Jiří; Louda, F.; Čítek, Jaroslav; Ducháček, Jaromír

doi:10.5194/aab-55-207-2012

Cited by 27 publications

(36 citation statements)

References 31 publications

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“…The last fi ndings confi rm that the initial quality of ejaculate determines fi nal quality of AI dose (Beran et al, 2012).…”

Section: Resultsmentioning

confidence: 64%

“…Collection of ejaculate and its subsequent processing present further potential risk factors for declined sperm quality. Phases of producing insemination doses (diluting of sperm, fi lling of straws, cooling and freezing) have a signifi cant eff ect on sperm motility a er thawing (Siddique et al, 2006), and the properties of extender used are especially important (Beran et al, 2012;Hegedüšová et al, 2012), as well as method of thawing (Filipčík and Hanuláková, 2011).…”

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confidence: 99%

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Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Beran¹,

Šimoník²,

Stádník³

et al. 2013

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The objectives of this study were to determine and evaluate the eff ect of bull, diluter and addition of LDL in diff erent concentration on the percentage rate of spermatozoa survival a er cold shock. In total, four bulls were collected during a period of eight weeks. A total of 8 samples of fresh semen with required quality were processed. Three extenders were used for dilution of each sample; AndroMed®, Bioxcell® and Triladyl®, each in standard and LDL enriched variants. In the case of AndroMed® and Bioxcell®, 4, 6 and 8% of LDL were simply added. In Triladyl®, 6, 8 and 10% of LDL replaced the standard egg yolk component. Resistance of spermatozoa against cold shock (0 °C, 10 minutes) was evaluated by the percentage rate of live sperm using Eosin-Nigrosine staining immediately and 2 hours a er heat incubation (37 °C). The results showed the infl uence of bull individuality as an important factor. Among diluters used it is possible to recommend AndroMed® and Bioxcell® due to signifi cantly (P < 0.01) lower decline of live sperm proportion during the cold shock test than Triladyl® (−9.19, respectively −4.95%). The optimal LDL concentration increasing resistance of spermatozoa against cold shock was not determined, therefore subsequent research is necessary. bull semen, sperm survival, cold shock, extender, LDL cholesterol

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“…The last fi ndings confi rm that the initial quality of ejaculate determines fi nal quality of AI dose (Beran et al, 2012).…”

Section: Resultsmentioning

confidence: 64%

mentioning

confidence: 99%

Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Beran¹,

Šimoník²,

Stádník³

et al. 2013

Acta Univ. Agric. Silvic. Mendelianae Brun.

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“…A phase contrast microscope with oil immersion (Eclipse E200, Nikon) at 1000× magnification was used by only one evaluator to examine all smears acquired. Minimally 100 spermatozoa were classified as either dead (with red heads) or live (with white heads), and expressed as a percentage rate of live sperm (Beran et al 2012).…”

Section: Methodsmentioning

confidence: 99%

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

Czech J. Anim. Sci.

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ABSTRACT:The objective was to determine effects of equilibration length and freezing curve type as well as their interactions on motility and live spermatozoa proportion in bull sperm after thawing. The ejaculates of 6 sires were repeatedly collected. Fresh semen was diluted with one extender and divided into 3 groups equilibrated for 30, 120, and 240 min. Subsequently, half straws of each group were frozen using standard 3-phase or 2-phase freezing curve differing in the rate of temperature decrease. Th e spermatozoa motility (M) was evaluated immediately after thawing and at 30, 60, 90, and 120 min of thermodynamic test (TDT). Live spermatozoa proportion was evaluated after thawing and at the end of TDT. Average of spermatozoa motility (AM), decrease of spermatozoa motility (MD), average proportion of live spermatozoa (ALS), and decrease of live spermatozoa proportion (DLS) through the TDT were calculated. Significant inter-sire differences in AM (0.45-17.0%; P < 0.05-0.01), MD (0.76-12.57%; P < 0.05-0.01), and ALS (0.99-23.8%; P < 0.01) were detected. The longest equilibration ensured the highest M during TDT and AM (+2.72 and +4.58%; P < 0.05-0.01), however higher MD (+4.06%; P < 0.01) compared to standard length as well. Straws freezed using 2-phase curve achieved higher M through TDT, AM (+7.3%; P < 0.01) as well as ALS (+11.77%; P < 0.01). The 2-phase curve presented higher M compared to the 3-phase freezing curve within all equilibration lengths. Significant differences in AM, MD,; P < 0.05-0.01) between equilibration length vs freezing curve interactions were determined. Results document the importance of equilibration length, freezing curve, and their interaction effect on live spermatozoa proportion and sperm motility after thawing as well as necessity of individual conditions for bulls semen processing and insemination doses production.

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“…As has been reported, cow conception rates may be influenced by the quality of AI doses (Beran et al, 2012) and quality of AI technicians' service (Walsh et al, 2011). In our work, only AI doses obtained from one sire were used and they were applied by one (the same) AI technician; this fact eliminates the possible influence of the sire´s individuality and service quality on the results of the performed AI.…”

Section: Discussionmentioning

confidence: 93%

The yield and cell viability of bovine <i>in vivo</i> recovered embryos in relation to season of flushing

Stádník¹,

Makarevich²,

Doležalová³

et al. 2014

J. Anim. Feed Sci.

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Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

Cited by 27 publications

References 31 publications

Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

The yield and cell viability of bovine <i>in vivo</i> recovered embryos in relation to season of flushing

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