Assessment of in vitro antioxidant capacity of the seed coat and the cotyledon of legumes in relation to their phenolic contents

Dueñas, Montserrat; Hernández, Teresa; Estrella, Isabel

doi:10.1016/j.foodchem.2005.05.052

Cited by 164 publications

(138 citation statements)

References 37 publications

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“…Our result displays similarity with the investigations of Hagerman, (1998) andFalleh, (2008), who reported that medicinal plants markedly scavenge free radicals. The results obtained by Duenas et al, (2006) and Kilani et al, (2008) also support our findings.…”

Section: Dpph Free Radical Scavenging Assaysupporting

confidence: 90%

Promising inhibition of krait snake’s venom acetylcholinesterase by <italic>Salix nigra</italic> and its role as anticancer, antioxidant agent

Ahmed

Ahmad

Khan

et al. 2016

IJAR

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Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. The available antivenoms are associated with numerous side effects. As an alternative, researchers have extensively studied the plants in order to obtain some unusual treatment. The current study was conceded to evaluate the effects of salix nigra (Black willow) against krait snake venom acetylcholinesterase and its role as anti-cancer, anti-oxidant agent. Standard protocols of Ellman, Meyer, Gyamfi and Ruch were used for enzyme inhibition, anti-cancer and free radicals scavenging assays respectively. The methanolic extract of Salix nigra proved to possess neutralization properties against krait snake (Bungarus Sindanus) venom acetylcholinesterase. Statistical data of the results showed that Salix nigra extract inhibited the krait venom acetylcholinesterase through concentration dependent manner. Kinetic analysis using Lineweaver Burk plot revealed that Salix nigra caused competitive type of inhibition i.e. k m value increased while V max remain constant with an increase of extract's concentration. The calculated IC 50 value of Salix nigra was found to be 177µg/ml. In DPPH free radical scavenging assay, extract showed good scavenging potential with IC 50 value of 317µg/ml. Similarly, using H 2 O 2 free radicals, the % scavenging of the extract at 100, 250, 500 and 1000µg/ml was 41, 54, 69 and 74% in comparison with ascorbic acid. Moreover, the extract exhibited good cytotoxic activity against brine shrimps in a dose dependent manner. The present work suggests, for the first time, that Salix nigra extract can be used not only as a potent inhibitor of snake venom acetylcholinesterase but also for the eradication of free radicals along with significant anticancer activity.

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Section: Dpph Free Radical Scavenging Assaysupporting

confidence: 90%

Promising inhibition of krait snake’s venom acetylcholinesterase by <italic>Salix nigra</italic> and its role as anticancer, antioxidant agent

Ahmed

Ahmad

Khan

et al. 2016

IJAR

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“…These compounds used in the treatment of chronic inflammatory diseases associated with overproduction of nitric oxide [46]. The toxicity of nitric oxide increases greatly when it reacts with superoxide radicals forming the highly reactive peroxynitrite anion (ONOOˉ) [47].…”

Section: Volume 14mentioning

confidence: 99%

Scavenging Activity, Anti-Inflammatory and Diabetes Related Enzyme Inhibition Properties of Leaves Extract from some Varieties of <i>Phoenyx dactylifera l.</i>

Eddine

Ladjel²,

Gherraf

et al. 2013

ILCPA

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In this study we investigate the antioxidant, anti-inflammatory, and antidiabetic activities of ethanolic leaves extracts of three selected varieties of Phoenyx dactylifera L. namely: "Ghars", "Deglet Nour" and "Hamraya". The assessment of the antioxidant potential of crude leaves extracts, using superoxide anions inhibition, DPPH and total antioxidant activity essays, was carried out. Furthermore, the anti-inflammatory properties of the extracts were determined by measuring the inhibition of nitric oxide (NO) production. Moreover, the antidiabetic effect was evaluated by inhibition of α-amylase enzymes. The total phenolic content measured by Folin-ciocalteu method was as well conducted. The raw leaves extracts of the selected varieties was found to contain a high content of total phenolic content (342.45 mg GAE/gDW for GE) and therefore exhibited a higher antioxidant activity and inhibitory effect of radicals scavenging activity against DPPH and superoxide anion (IC 50 = 7.44 μg/mL and 39.11 μg/mL respectively). The three varieties exhibited significant inhibition nitric oxide using in-vitro assay (IC 50 = 240.28 μg/mL for GE). The extracts also displayed high inhibition actions against α-amylase enzymes. The results suggest that the leaves of the three selected varieties of Phoenyx dactylifera can be considered as a good source of natural antioxidant and anti-inflammation drugs as well as potent antidiabetic medicine.

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“…Other authors have observed high antioxidant activity in lentils, faba beans and peas due to the essential fact that large amount of phenolic compounds are located in the seed coat [10]. Duenas [11] described the distribution of phenolic compounds in different parts of the legume seeds, the cotydelon contains the main reserve substances and the seed coat has the highest concentration of phenolic compounds. CevallosCasals and Cisneros-Zevallos analyzed the levels of phenolic compounds and the antioxidant activity of thirteen edible seed species at different germination stages.…”

Section: Introductionmentioning

confidence: 98%

The impact of LED illumination on antioxidant properties of sprouted seeds

Samuolienė¹,

Urbonavičiūtė²,

Brazaitytė

et al. 2010

Open Life Sciences

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Abstract:The objective of this study was to evaluate the effect of the light emitting diode (LED) spectra on the antioxidant properties of sprouted wheat (Triticum aestivum L.), radish (Raphanus sativus L.), and lentil (Lens esculenta Moenh.) seeds. Lighting experiments were performed under controlled conditions (PPFD -100 µmol m -2 s -1 ; 12 h photoperiod; 27°C). The LED conditions used were: L1 -638 nm; L2 -455 nm, 638 nm, 669 nm, 731 nm (basal components); L3 -basal + 385 nm; L4 -basal + 510 nm and L5 -basal + 595 nm. Wheat and lentil sprouts were shown to accumulate less phenolic compounds and were more sensitive to light spectral differences when compared to radish sprouts. The antioxidant properties and contents of antioxidant compounds in seeds germinated in the dark were significantly lower than LED treated seeds. The higher content of total phenols and significant increase in alpha-tocopherol and vitamin C concentration resulted in altered DPPH free-radical scavenging capacity. Therefore we conclude that the LED spectra, based on basal components supplemented with green (510 nm) light can improve the antioxidant properties of sprouted seeds of lentil and wheat. The highest antioxidant properties of radish seeds were caused by radiation with supplemental amber (595 nm) light.

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Assessment of in vitro antioxidant capacity of the seed coat and the cotyledon of legumes in relation to their phenolic contents

Cited by 164 publications

References 37 publications

Promising inhibition of krait snake’s venom acetylcholinesterase by <italic>Salix nigra</italic> and its role as anticancer, antioxidant agent

Promising inhibition of krait snake’s venom acetylcholinesterase by <italic>Salix nigra</italic> and its role as anticancer, antioxidant agent

Scavenging Activity, Anti-Inflammatory and Diabetes Related Enzyme Inhibition Properties of Leaves Extract from some Varieties of <i>Phoenyx dactylifera l.</i>

The impact of LED illumination on antioxidant properties of sprouted seeds

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