Assessment of in vitro antimicrobial potency and free radical scavenging capacity of the essential oil and ethanol extract of Calycotome villosa subsp. intermedia growing in Algeria

Abstract

International audienceObjective: To assess the antimicrobial and antioxidant activity of the essential oil and ethaniol extract of the aerial parts of Calycotome villosa subsp. intermedia growing in the West Northern region of Algeria. Methods: Chemical composition of essential oils obtained by hydrodistillation from areal parts of Calycotome villosa subsp intermedia was investigated using gas chromatography (retention indices) and gas chromatography-mass spectrometry while the antimicrobial activities were determinate by paper disc diffusion method and minimum inhibitory concentration assay tested against four bacterial strains and one yeast and antioxidant activity was evaluated as a free radical scavenging capacity (RSC). Results: Essential oils were dominated by non-terpenic compounds and fatty acids. Howeverthe phenylpropanoid monoterpenes and sesquitepene components were only present in small percentages. The most important antibacterial activity of essential oil was expressed on Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae and Salmonella typhimurium. Antioxidant activity was evaluated as a RSC. RSC was assessed by measuring the scavenging activity of essential oil and ethanol extract on 1,1-diphenyl-2-picrylhydrazil (DPPH). Investigated ethanol extract reduced the DPPH radical formation (IC50=68 μg/mL). Conclusions: Results in this experiment indicate that the essential oil and the ethanol extract display antibacterial activity against two Gram-positive bacteria and activity to a lesser extent against two Gram-negative species. They may be a new potential source of components, which ar likely to have impact on human health

Phytochemical analysis, antioxidant and antimicrobial activities of leaves and flowers ethyl acetate and n-butanol fractions from an Algerian endemic plant Calycotome spinosa (L.) Link

“…According to Chikhi et al (2014), the two extracts of C. villosa leaves (essential oil and ethanol) showed a good antioxidant/free radical scavenging activity using DPPH method. The weakest radical scavenging capacity was exhibited by the essential oil (60%), whereas the strongest activity was exhibited by the ethanol extract (96%) at a concentration of 200 µg/ml when compared with the effect of ascorbic acid at this concentration (98.61%).…”

“…According to Chikhi et al (2014), the in vitro antimicrobial activities of essential oil and ethanol extracts of C. villosa showed an important activity. A high activity was observed by essential oil with antimicrobial inhibition zones of 14 and 13 mm against S. aureus and E. faecalis, respectively and low activity against K. pneumoniae and Salmonella typhimurium with an antimicrobial inhibition zone of 11 mm.…”

“…As a part of a systematic research study on the constituents, antioxidant activity and antimicrobial potential of Algerian natural plants, the phytochemical and biological properties of Calycotome villosa (Chikhi et al, 2014;Djeddi et al, 2015) and Retama raetam (Djeddi et al, 2013) have already been reported.…”

New Compounds from the Roots of Corsican Calicotome Villosa (Poir.) Link.: Two Pterocarpans and a Dihydrobenzofuran

“…intermedia is distributed especially in the North of Africa and Spain [ 3 ]. Solvent extracts, essential oil, and isolated compounds from C. villosa showed diverse biological activities including antioxydant, antimicrobial, anti-inflammatory, antidiabetic, hypotensive, diuretic, and vasodilatator [ 4 , 5 , 6 , 7 ]. Phytochemicals that belong to various families have been isolated and identified in C. villosa extracts from different parts of the plant, including alkaloids, flavonoids, steroids, anthraquinones, and phenol derivatives [ 2 , 3 , 4 , 7 , 8 , 9 , 10 , 11 , 12 ].…”

“…Phytochemicals that belong to various families have been isolated and identified in C. villosa extracts from different parts of the plant, including alkaloids, flavonoids, steroids, anthraquinones, and phenol derivatives [ 2 , 3 , 4 , 7 , 8 , 9 , 10 , 11 , 12 ]. In addition, falcarinol (fatty alcohol), some oxygenated mono- and sesquiterpenes, furan derivatives, paraffins, and fatty acids have been identified in the essential oil from aerial parts of C. villosa [ 2 , 4 , 5 ]. Concerning root extracts, only one paper reported on the screening of total alkaloid, saponin, and flavonoid contents [ 13 ].…”