Assessment of immunization response to caninedistemper virus vaccination in puppies using a clinic-based enzyme-linked immunosorbant assay

Waner, Trevor; Naveh, Ami; Meir, N. Schwarz Ben; Babichev, Z.; Carmichael, L E

doi:10.1016/s1090-0233(98)80013-0

Cited by 19 publications

(16 citation statements)

References 3 publications

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“…This correlated well with what was reported in literature (Abdullahi, 1979). Investigation of immune status following vaccination using standard procedures like the hemagglutination inhibition (HI), serum neutralization (SN) and immunofluorescent antibody (IFA) has not been practicable in Nigeria in view of the cost and other limitations associated with these tests (such as trained personnel and time constraint) as has been the case even in some advanced countries of the world (Waner et al, 1996;Waner et al, 1998;Waner et al, 2004). Thus, the use of a rapid inclinic immunoblot ELISA technique for the semi quantitative analyses of antibody titers to CPV and CDV provides solution to this limitation.…”

Section: Discussionsupporting

confidence: 81%

“…Phone: +2348037240874 or +2348053910615 the use of modified-live CDV vaccines and the development of several diagnostic tests for specific viral diagnosis. Waner et al (1998) reported that the persistence of canine distemper is as a result of insufficient vaccinetions in cases where multiple vaccinations are a common practice and or vaccination failures probably due to faulty vaccine and interference by maternally derived antibody (MDA).…”

Section: Introductionmentioning

confidence: 99%

“…Veterinarians and researchers have come to the conclusion that the surest way to know that a puppy has adequately responded to vaccination or to confirm the immune status in a mature dog is to check the antibody levels in the dog's blood or serum. An easily accessible in-clinic procedure called immunoblot ELISA assay (rapid dot-ELISA assay or immunocomb ELISA test) has been developed for the semi-quantitative assay of CDV and CPV IgG antibody titres in the sera of vaccinated mature and young dogs using the enzyme-linked immunosorbent assay (ELISA) technology (Naveh et al, 1995;Waner et al, 1996;Waner et al, 1998;Truyen, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of post-vaccination immunity to canine distemper and parvoviruses in Benin City, Nigeria

Eghafona¹,

N²,

Jacob³

et al. 2007

Afr. J. Biotechnol.

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A study was conducted to determine the immune status of dogs vaccinated against Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) by a clinic-based immunoblot enzyme-linked immunosorbent assay (ELISA) using a commercially available 120 sample immunocomb® test kit for canine parvo and distemper IgG in Benin City, Nigeria. Out of 120 dogs sampled, 63 (52.5%) were females while 57 (47.5%) were males. 84 (70%) dogs were exotic breeds while 36 (30%) were mongrels. Majority of the dogs (35, 29.17%) were less than one year old and the age range of all dogs sampled was between 9 weeks and 10 years. Analysis of data showed that there was no significant difference (P < 0.05) between sex, breed, age and level of immunity. A non-parametric chi-square contingency analysis of antigens (CPV and CDV) and level of immunity revealed that there was a high significant association (p < 0.001) between CPV and CDV antigens and level of immunity of the vaccinated dogs sampled.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of post-vaccination immunity to canine distemper and parvoviruses in Benin City, Nigeria

Eghafona¹,

N²,

Jacob³

et al. 2007

Afr. J. Biotechnol.

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“…By extrapolation an ''S'' value of 1 represented a titer of about 1:20 and an ''S'' value of 2 was equivalent to a titer of about 1:40. 12,13 For statistical analysis dogs were categorized into 6-montly intervals according to their last vaccination and mean serum antibody titers for CPV and CDV calculated for each interval. A one-way ANOVA was used to compare the mean antibody titers for CPV and CDV for the different time intervals, and the level of significance was set at P # 0.05.…”

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confidence: 99%

Application of a Dot Enzyme-Linked Immunosorbent Assay for Evaluation of the Immune Status to Canine Parvovirus and Distemper Virus in Adult Dogs before Revaccination

2006

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Abstract. A growing body of literature has been published indicating that the current practice of annual vaccination of dogs may not be beneficial and in some cases may even be harmful. A number of publications have proposed assessing the immune status of dogs before annual revaccination. In this study the usefulness of a commercially available dot-ELISA kit was evaluated to determine the duration IgG antibody titers to canine parvovirus (CPV) and canine distemper virus (CDV) in 158 dogs vaccinated at least one year ago. Overall, the percentage of dogs with protective antibody titers to both CPV and CDV was 84%. The percentage of dogs with borderline antibody titers was 11% for CPV and 10% for CDV. Four percent of the dogs had no detectable antibody to CPV and 6% had no antibody to CDV. The results reported here are in good agreement with other studies measuring IgG antibody levels. It is concluded that the kit offers veterinarians the opportunity of determining antibody titers and revaccinating only those pets whose antibody titers to specific diseases have waned.

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“…In the field of canine medicine, this technique has recently been successfully used for the detection of serum immunoglobulin G (IgG) antibodies to canine parvo virus and distemper virus. 17,18 The technique requires a minimum of equipment, is easy and quick to perform, and involves a single-step dilution.…”

mentioning

confidence: 99%

Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Test for the Assay of Ehrlichia Canis Antibodies in Dogs

2000

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Abstract. The ''gold standard'' for the detection of antibodies to Ehrlichia canis, the cause of canine monocytic ehrlichiosis (CME), is the indirect immunofluorescence antibody (IFA) test. The IFA test however is generally available only in selected laboratories and requires extensive equipment and trained personnel. A double-blind study was conducted to compare the ability of an in-clinic standardized enzyme-linked immunosorbent assay (ELISA) test kit to measure E. canis IgG antibodies in dogs compared with the standard IFA technique. A good correlation was found between the 2 techniques (r 2 ϭ 0.8793; P Ͻ 0.0001). Evidence for the sensitivity of the ELISA technique for the early detection of E. canis IgG antibodies was demonstrated by comparing the appearance of E. canis antibody titers by the IFA and ELISA techniques after artificial infection of 2 sets of dogs. In both experimental infections, both tests were equally sensitive for the early detection of IgG antibodies against E. canis, and the results correlated well with the appearance of fever and clinical signs. Proposed application of the in-clinic ELISA test is to aid in the diagnosis of CME.

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Assessment of immunization response to caninedistemper virus vaccination in puppies using a clinic-based enzyme-linked immunosorbant assay

Cited by 19 publications

References 3 publications

Evaluation of post-vaccination immunity to canine distemper and parvoviruses in Benin City, Nigeria

Evaluation of post-vaccination immunity to canine distemper and parvoviruses in Benin City, Nigeria

Application of a Dot Enzyme-Linked Immunosorbent Assay for Evaluation of the Immune Status to Canine Parvovirus and Distemper Virus in Adult Dogs before Revaccination

Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Test for the Assay of Ehrlichia Canis Antibodies in Dogs

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