Assessment of genetic relationships among common buckwheat (Fagopyrum esculentum Moench) varieties from Western Balkans using morphological and SSR molecular markers

Abstract

In order to assess the genetic relationships and diversity among common buckwheat from Western Balkans, three varieties produced under the names 'Ĉebelica', 'Darja' and 'Goluba' were evaluated with a set of 10 SSR and 32 morphological markers. Eight of ten primer pairs used managed to amplify SSR alleles, in average 7 alleles per locus. Analysis of molecular variance (AMOVA) revealed that only 2.6% of the total diversity was attributed to the differences among 'Darja' and 'Goluba'. The largest percentage of variance between varieties was detected among 'Goluba' and 'Ĉebelica' (f CT = 0.136; p < 0.001). Factorial correspondence analysis also revealed a clear differentiation between these two varieties. Results of hierarchical clustering based on morphological data of the three analyzed common buckwheat varieties were not in a complete accordance with the 792

Genetic Relationships and Diversity of Common Buckwheat Accessions in Bosnia and Herzegovina

“…On the other hand, statistically significant differentiation was found between 'Goluba' and 'Čebelica' (FCT = 0.112; p < 0.0170) and 'Darja' and 'Čebelica' (FCT = 0.112; p < 0.0170). These results have previously been reported by Grahić et al [21] in a study that focused on only these three varieties, without a wider context of buckwheat landraces from Bosnia and Herzegovina.…”

“…This is probably due to the use of a narrow genepool for buckwheat breeding in Slovenia. High similarity between 'Goluba' and 'Darja' was already reported by Grahić et al [21] in a study which focused on the genetic relationships of common buckwheat varieties from the Western Balkans. On the other hand, the third reference variety, 'Čebelica', is clearly separated from the other two.…”

“…Therefore, a combination of SSRs and morphological descriptors represents the most encompassing approach in the assessment of genetic resources among crops such as common buckwheat. Microsatellites have previously been successfully used in several common buckwheat germplasm studies [5,6,[18][19][20], while the combined approach of SSRs and morphological descriptors is far rarer [21,22]. Therefore, the aim of this study was to assess the genetic relationships and diversity of the common buckwheat accessions maintained at the FAFS Gene bank, as well as the value of these accessions for future breeding programs.…”

Germinated Buckwheat: Effects of Dehulling on Phenolics Profile and Antioxidant Activity of Buckwheat Seeds

“…However, to the best of our knowledge, there have not been any studies into the effects of buckwheat seed dehulling on the germination bioactivities. Furthermore, for the Slovenian domestical cultivar of common buckwheat ( Fagopyrum esculentum ) “Čebelica” [ 36 ] used here, there appears to be no information on their phenolic profile.…”