Assessment of genetic fidelity and composition: Mixed elicitors enhance triterpenoid and flavonoid biosynthesis of <i>Glycyrrhiza uralensis</i> Fisch. tissue cultures

Wang, Juan; Li, Jing; Wu, Xiao‐Lei; Liu, Shujie; Li, Hongfa; Gao, Wenyuan

doi:10.1002/bab.1485

Cited by 14 publications

(2 citation statements)

References 36 publications

(52 reference statements)

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“…Figure 6B shows the detailed process of breaking down the mass spectrum of the negative ion of glycyrrhizic acid in a similar manner. Analysis of the mass spectral cleavage pattern of glycyrrhizic acid and comparison with the literature data 33–35 resulted in the identification of a series of additional peaks one by one.…”

Section: Resultsmentioning

confidence: 99%

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Preliminary investigation on the analysis of the whole components of Pogejiuxin decoction and its formulation pattern based on ultrahigh‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Zhang,

Xiang,

Fan

et al. 2024

Rapid Comm Mass Spectrometry

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RationalPogejiuxin decoction (PGJXD) is one of the most important formulas for the treatment of heart failure. However, there is a great lack of research on the material basis of this formula, especially research on its compatibility laws, which restricts its clinical use. Studying the complete ingredients and compatibility rules of PGJXD has great significance for guiding clinical medication.MethodsThe entire formula, the major single herbs, the drug pairs and the disassembled formula were analyzed by ultrahigh‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry (UHPLC/QTOFMS/MS), matching the chemical composition database and global natural product social molecular networking to explain the chemical composition as well as the combination pattern of PGJXD.ResultsA total of 1048 chemical constituents were fully analyzed from the major single herbs, the drug pairs and the disassembled formula and 188 chemical constituents, including 13 potential novel compounds, were firstly identified from the whole formula. We found that the chemical compositions were reduced after the single herbs were matched to the other herbs, especially the significant reduction of highly toxic diester alkaloids after compatibility, indicating that the medicines of PGJXD were interdependent and controlled by each other.ConclusionThis study innovatively researches and compares the compositional differences between the entire formula of PGJXD, the single, paired and separated formulas, greatly extending our understanding of the chemical substance basis of these compounds, and preliminarily explores the compatibility laws of PGJXD, providing some theoretical guidance for clinical medication.

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Section: Resultsmentioning

confidence: 99%

“…Analysis of the mass spectral cleavage pattern of glycyrrhizic acid and comparison with the literature data [33][34][35] resulted in the identification of a series of additional peaks one by one. and are presumed to be potential new compounds.…”

Section: Triterpenoid Structural Analysismentioning

confidence: 95%

Preliminary investigation on the analysis of the whole components of Pogejiuxin decoction and its formulation pattern based on ultrahigh‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Zhang,

Xiang,

Fan

et al. 2024

Rapid Comm Mass Spectrometry

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Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress

Mosavat

Golkar

Yousefifard

et al. 2019

Biotech and App Biochem

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Thymus species are aromatic plants with diverse applications in food industries and medicine. This study was conducted to evaluate the potential effect of ZnO nanoparticles (NPs) on callus proliferation and thymol and carvacrol production in three Thymus species, that is, T. vulgaris, T. daenensis, and T. kotschyanus, and Zataria multiflora. For this purpose, callus induction was performed on Murashige and Skoog (MS) medium containing different plant growth regulators (PGRs). After optimization of callus growth, the effects of different concentrations of ZnO NPs (100 and 150 mg L −1 ) were investigated. MS containing 2 mg L −1 of 2, 4-dichlorophenoxy acetic acid (2,4-D) and 1 mg L −1 of kinetin (Kin) revealed significantly highest fresh weight (0.18 g) of callus in T.kotschyanus. Callus growth rate (0.079 mm day −1 ) was found highest in T. vulgaris under similar conditions. Moreover, highest callus induction (92.50%) was achieved by T. kotschyanus in MS containing 2.5 mg L −1 of 2,4-D. Regarding the highest content of thymol (22.8 mg L −1 ) and carvacrol (0.68 mg L −1 ) evaluated by high-performance liquid chromatography, best results were achieved under 150 mg L −1 of ZnO NPs in T. kotschyanus and T. daenesis, respectively. This is simple and cost-effective method to be applied on industrial level for production of enhanced secondary metabolites content. potent chemical elicitors as signaling agents that effect a great diversity of physiological and biochemical processes in plant metabolism and plant secondary metabolites production [1,2]. NPs are capable of increasing cell membrane permeability, stimulating defensive mechanisms in plant cells, and subsequent increase in secondary metabolites content [2,3]. Zinc oxide (ZnO) NPs as an inorganic micronutrient have a beneficial effect on plant growth, cell division, and structure and function of membrane because of their high electron mobility, large surface area, and catalytic activity [4]. Most of the recent studies are focused on the effects of NPs on seed germination [5], plant growth rate [6] physiological responses [3][4][5][6], and secondary metabolites production [2][3][4][5]. Recent studies have shown that plant growth, development, and physiology are significantly affected by NPs; however, their role on plant secondary metabolism, especially regarding callus culture, is still obscure [2]. The inclusion of NPs to tissue culture media could enhance

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Integrated morphology analysis, metabolomic analysis and gene expression to assess the quality of four adventitious roots lines of Glycyrrhiza uralensis Fisch

Lü

Liang

et al. 2018

Plant Cell Tiss Organ Cult

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Assessment of genetic fidelity and composition: Mixed elicitors enhance triterpenoid and flavonoid biosynthesis of Glycyrrhiza uralensis Fisch. tissue cultures

Cited by 14 publications

References 36 publications

Preliminary investigation on the analysis of the whole components of Pogejiuxin decoction and its formulation pattern based on ultrahigh‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Preliminary investigation on the analysis of the whole components of Pogejiuxin decoction and its formulation pattern based on ultrahigh‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress

Integrated morphology analysis, metabolomic analysis and gene expression to assess the quality of four adventitious roots lines of Glycyrrhiza uralensis Fisch

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