Assessment of genetic diversity in maize inbred lines using RAPD markers

Bruel, Daniela Cristina; Carpentieri-Pípolo, Valéria; Ruas, Claudete de Fátima; Gerage, Antônio Carlos; Souza, S. G. H. de

doi:10.12702/1984-7033.v07n02a09

Cited by 9 publications

(12 citation statements)

References 3 publications

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“…However, the rate of polymorphisms found in the present study was similar to that observed by Bruel et al (2007) and Lanza et al (1997). Carvalho et al (2004), Moeller and Schaal (1999), and Rinaldi et al (2007) have obtained lower polymorphic indices ranging from 71 to 76%.…”

Section: Discussionsupporting

confidence: 90%

“…Several methods have been applied in this regard: random amplified polymorphic DNA (RAPD) (Williams et al, 1990), simple sequence repeat (SSR) (Tautz, 1989), and amplified fragment length polymorphism (AFLP) (Zabeau and Vos, 1993). RAPD is widely used in studies of maize diversity owing to its low cost and rapid detection of polymorphisms (Carvalho et al, 2004;Bruel et al, 2007). SSR features high reproducibility, accuracy, and discrimination.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers

Molin¹,

Coelho²,

Máximo³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Maize landraces derived from tropical germplasm represent an important source of genetic variability, which is currently poorly understood and under-exploited by Brazilian crop breeding programs. The aims of our study were to a) estimate the genetic diversity across 48 varieties of maize landraces cultivated at different locations in the States of Rio Grande do Sul (RS) and Paraná (PR) by means of random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers; b) cluster these varieties based on their genetic similarity estimates, and c) establish possible correlations between genetic similarity and germplasm collection sites. Maize landrace accessions were genotyped through the 30 RAPD, 47 SSR, and 25 combinations of AFLP primers. The results revealed high levels of variability across landraces within and between collection sites. AFLP analysis resulted in amplification of 762 polymorphic fragments and a polymorphic index of 40.3%, followed by RAPD with 335 fragments (81.9%) and SSR with 105 fragments (78.3%). The genetic similarity estimates of the investigated landraces ranged from 41 (SSR) to 74% (AFLP), and the amplitudes of these indices were notably similar between RAPD and SSR, as well as between AFLP and joint analysis. Regarding the RAPD and AFLP dendrograms, groups comprising accessions from RS prevailed, whereas SSR comprised varieties from both collection sites. Groups exclusive to RS or PR support the hypothesis that divergence between groups is possible owing to the fixation of regional adaptation alleles and to spatial barriers hindering genetic flow between locations.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers

Molin¹,

Coelho²,

Máximo³

et al. 2013

Genet. Mol. Res.

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“…Different morphological, biochemical, and molecular markers are utilized by the breeders to identify the genetic diversity among the accessions (Pejic et al, 1998;Bauer et al, 2005). However, a highly informative, cost-and time-saving technique (molecular genetic markers) has replaced all 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 other techniques (Roder et al, 1998;Carvalho et al, 2004;Bruel et al, 2007). Among these markers, SSRs have more importance because of their codominant characteristics (Pejic et al, 1998;Tautz, 1989).…”

Section: Discussionmentioning

confidence: 99%

Sources of genetic resistance in maize to Fusarium stalk rot andtheir variations at molecular level

Qureshi¹,

Qayyum²,

Fiers³

2015

Turk J Agric For

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IntroductionMaize germplasm has the potential to exhibit many genetic variations that help it to survive in a wide range of environments (Paterniani et al., 2000). Genetic diversity is desirable in all breeding programs and landraces are an important source for tolerant or resistant genes against biotic and abiotic stresses (Araújo and Nass, 2002). Landraces are continuously replaced by high-yielding cultivars having narrower genetic variations (Pollack, 2003), while genetic enhancement of germplasm pools is only possible by exploring wider genetic resources (Rajaram and Van Ginkel 1996).Stalk rot disease of maize, caused by Fusarium verticillioides, is one of the most widespread and damaging diseases throughout the world, resulting in serious yield losses (

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“…relationships among them. In the case of maize, there are a lot of papers (Osipova et al, 2003;Carvalho et al, 2004, Asif et al, 2006Bruel et al, 2007, Abuali et al, 2011, Al-Badeiry et al, 2013, Molin et al, 2013 that have reported the application of the RAPD marker technique for maize molecular identification, and the technique was proved to be effective for or verification of purity and would improve the efficiency of breeding programmes (Asif et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of molecular diversity of central European maize cultivars

Balážová

Vivodík

Gálová

2016

Emir. J. Food Agric

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Assessment of genetic diversity in maize inbred lines using RAPD markers

Abstract: -RAPD molecular markers were used to analyze genetic diversity between

Cited by 9 publications

References 3 publications

Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers

Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers

Sources of genetic resistance in maize to Fusarium stalk rot andtheir variations at molecular level

Evaluation of molecular diversity of central European maize cultivars

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