Assessment of genetic diversity in cassumunar ginger (Zingiber cassumunar Roxb.) in Thailand using AFLP markers

Abstract

The genetic relationship among cassumunar gingers (Zingiber cassumunar) in Thailand was assessed by amplified fragment length polymorphism (AFLP). Twelve AFLP primer combinations generated a total of 309 fragments, of which, 242 bands were polymorphic with an average of 20.2 bands per primer pair. Genetic similarities were obtained using Jaccard similarity coefficients, and a phylogenetic tree was constructed using the UPGMA clustering method. Pairwise similarity estimated between cassumunar gingers ranged from 0.7644 to 1.00 with an average of 0.879. Cluster analysis divided the samples into five groups with a high co-phenetic correlation value (r = 0.99). Genetic variability within and among collection regions was estimated by analysis of molecular variance (AMOVA). High molecular variance (84%) was found within samples from the same region. The results implied dispersal of plant materials between collection regions. The genetic similarity assessed by AFLP showed that there are duplicate accessions in the germplasm collection. This genetic information is very useful for germplasm maintenance and a crop improvement program.

Morphological and molecular characterization of underutilized medicinal wild ginger (Zingiber barbatum Wall.) from Myanmar

“…(Velayudhan et al, 1999;Sasikumar, 2005;Hussain et al, 2008;Keeratinijakal et al, 2010), Alpinia spp. (Ravindran et al, 1994;Kladmook et al, 2010), as well as other crop species such as kale (Cartea et al, 2002), Vitis vinifera (Ortiz et al, 2004), greater yam (Hasan et al, 2008), watermelon (Szamosi et al, 2009), melon (Oumouloud et al, 2009), wheat (Dos Santos et al, 2009) and cowpea (Ghalmi et al, 2010). (Ravindran et al, 1994;Kladmook et al, 2010), as well as other crop species such as kale (Cartea et al, 2002), Vitis vinifera (Ortiz et al, 2004), greater yam (Hasan et al, 2008), watermelon (Szamosi et al, 2009), melon (Oumouloud et al, 2009), wheat (Dos Santos et al, 2009) and cowpea (Ghalmi et al, 2010).…”

Topical Niosome Gel of Zingiber cassumunar Roxb. Extract for Anti-inflammatory Activity Enhanced Skin Permeation and Stability of Compound D

“…Since ZC could be affected by cultivation conditions (21) or genetic diversity (22), yields of compound D are monitored to assure product quality. The samples of ZC gave about 15% yields of compound D which were insignificantly different between the collections from dry and rainy seasons (p>0.05).…”

“…Cultivation could have effects on the quality of ZC (21,22); thus, fresh rhizomes of Z. cassumunar from local farms were collected from 12 to 18 months old (about 300 g/ piece) with dark brown peel and yellowish bulk. The rhizomes were peeled, sliced, dried (50±1°C, 3 days) and ground into ZC powder.…”

“…(ZC), family Zingiberaceae, are usually applied topically onto the skin in Thai traditional medicine and spa treatment to alleviate musculoskeletal pain, rheumatism, and localized inflammation (14)(15)(16)(17)(18). (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol, comp o u n d D , o n e o f t h e p h a r m a c o l o g i c a l l y a c t i v e phenylbutanoids from ZC, exerts anti-inflammatory action (19,20) via cyclooxygenase inhibition and antioxidation (21,22) with cytochondroprotective activities which are advantageous for the treatment of arthritis (23)(24)(25)(26). Topical products of ZC are generally prepared in emulsions or gels (27)(28)(29) from crude extracts which provide greater anti-inflammatory potency than its isolated components (21).…”

Genetic Diversity Analysis of Heliconia psittacorum Cultivars and Interspecific Hybrids Using Nuclear and Chloroplast DNA Regions

“…Genetic diversity studies grew up in interest during the last years (Jatoi et al, 2008;Kladmook et al, 2010). As a result, nucleotide sequences of ribosomal genes (rDNA) and chloroplast genes (cpDNA) have been exploited to investigate several individuals of the Zingiberales order (Kress, 1990(Kress, , 1995Kress et al, 2001) once they are not capable of lateral transfers and are not subject to the same functional limitations, they allow greater confidence in the results (Camara, 2008).…”