Assessment of Genetic Diversity in Ethiopian Sesame (Sesamum indicum L.) Germplasm Using ISSR Markers

Abate, Mohammed; Mekbib, Firew; Ayana, Amsalu; Nigussie, M.

doi:10.9734/bbj/2015/18481

Cited by 5 publications

(8 citation statements)

References 20 publications

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“…In the present study, PIC varied from 0.002 to 0.35, indicating that ISSR markers are informative and relevant for discriminating the populations evaluated. However, these values remain lower than those obtained by Anitha et al (2010) in 10 Indian sesame populations (from 0.496 to 0.854), Abate et al (2015) in Ethiopian sesame (from 0.26 to 0.76) and Singh et al (2015) in 44 Indian sesame populations (PIC = 0.675). The differences observed among the various studies might be due to the populations used, and the nature and number of molecular markers.…”

Section: Genetic Relationship Among Moroccan Sesame Populations Accorcontrasting

confidence: 52%

“…This number varies according to plant species analyzed and differs from one study to another. The number of primers used in the present research was just seven, which was equal to that used for analyzing the genetic diversity in Ethiopian Sesame germplasm (Abate et al, 2015). However, smaller number of ISSR primers was adopted to study the genetic diversity in other annual species, for example five primers in Brassica napus (Abdelmigid, 2012) and Melocanna baccifera (Nilkanta et al, 2017).…”

Section: Genetic Relationship Among Moroccan Sesame Populations Accormentioning

confidence: 99%

“…Particularly, DNA markers are useful and reliable as they remain stable under different environmental conditions (Ferdinandez et al, 2001;Dar et al, 2019b). Genetic diversity in sesame over the world has been assessed using different molecular techniques namely, RAPD (Random Amplified Polymorphic DNA) (Salazar et al, 2006;Dar et al, 2017), AFLP (Amplified Fragment Length Polymorphism) (Laurentin and Karlovsky, 2006), ISSR (Inter Simple Sequence Repeats) (Kim et al, 2002;Kumar and Sharma, 2011;Nyongesa et al, 2013;Abate et al, 2015;Woldesenbet et al, 2015), SSR (Simple Sequence Repeat) (Uncu et al, 2015;Sehr et al, 2016;Dossa et al, 2017), and SRAP (Sequence Related Amplified Polymorphism) (Zhang et al, 2010). ISSR are DNA based markers having primers with simple repetitive sequences of two, four or five nucleotides, and known to be simple, fairly stable and highly reproducible (Houmanat et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers

Harfi

Charafi²,

Houmanat³

et al. 2021

OCL

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There is a need for sesame (Sesamum indicum L.) breeding in Morocco to release performant and adapted varieties, which requires a large genetic variability in the germplasm to be used. In this context, genetic diversity of sesame populations from different locations in Tadla area was investigated using ISSR (Inter Sequence Simple Repeats) markers as a simple method to reveal polymorphism among them. A total of 130 individuals representing 31 populations were sampled. Twenty-four ISSR primers were used for analysis of individuals representing the 31 different sesame populations grown in different agroclimatic zones of Tadla, accounting for 90% of sesame cultivation area in Morocco. Indeed, seven primers showed legible and reproducible genomic profiles with an interesting number of bands. A total of 57 bands were obtained with ISSR primers, of which 47 were polymorphic. PIC (Polymorphic Information Content) ranged from 0.002 to 0.350, showing that ISSR markers are informative and relevant for discriminating the populations evaluated. The similarity coefficient of ISSR data ranged from 0.509 to 1, with an average of 0.870. The results obtained showed that Moroccan sesame populations are characterized by a low genetic diversity, suggesting a genetic proximity among them. Therefore, new germplasm should be either introduced from diverse geographical origins or created through mutagenesis breeding in order to broaden the existing genetic variability.

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Section: Genetic Relationship Among Moroccan Sesame Populations Accorcontrasting

confidence: 52%

Section: Genetic Relationship Among Moroccan Sesame Populations Accormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers

Harfi

Charafi²,

Houmanat³

et al. 2021

OCL

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“…Pathak et al (2014) have previously reviewed in detail the numerous genetic diversity studies conducted on sesame germplasms. It emerges that sesame harbors a valuable diversity and incongruence between geographical proximity and genetic distance has been reported (Bhat et al, 1999; Laurentin and Karlovsky, 2006; Abdellatef et al, 2008; Pham et al, 2009; Zhang Y. et al, 2012; Alemu et al, 2013; Abate et al, 2015). However, few informative and polymorphic markers were used in these studies and might not accurately distinguish the samples.…”

Section: Application Of Newly Developed “Omics” Tools In Sesame Researchmentioning

confidence: 99%

The Emerging Oilseed Crop Sesamum indicum Enters the “Omics” Era

et al. 2017

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Sesame (Sesamum indicum L.) is one of the oldest oilseed crops widely grown in Africa and Asia for its high-quality nutritional seeds. It is well adapted to harsh environments and constitutes an alternative cash crop for smallholders in developing countries. Despite its economic and nutritional importance, sesame is considered as an orphan crop because it has received very little attention from science. As a consequence, it lags behind the other major oil crops as far as genetic improvement is concerned. In recent years, the scenario has considerably changed with the decoding of the sesame nuclear genome leading to the development of various genomic resources including molecular markers, comprehensive genetic maps, high-quality transcriptome assemblies, web-based functional databases and diverse daft genome sequences. The availability of these tools in association with the discovery of candidate genes and quantitative trait locis for key agronomic traits including high oil content and quality, waterlogging and drought tolerance, disease resistance, cytoplasmic male sterility, high yield, pave the way to the development of some new strategies for sesame genetic improvement. As a result, sesame has graduated from an “orphan crop” to a “genomic resource-rich crop.” With the limited research teams working on sesame worldwide, more synergic efforts are needed to integrate these resources in sesame breeding for productivity upsurge, ensuring food security and improved livelihood in developing countries. This review retraces the evolution of sesame research by highlighting the recent advances in the “Omics” area and also critically discusses the future prospects for a further genetic improvement and a better expansion of this crop.

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“…Also, environmental factors do not influence them, and DNA can be analyzed from any growth stage ( Dar et al, 2019b ; Abd El-Moneim et al, 2021 ). Several marker systems have been utilized to investigate the genetic variability and phylogenetic associations between sesame cultivars, including Random Amplified Polymorphic DNA (RAPD) ( Pham et al, 2009 ; Dar et al, 2017 ); inter simple sequence repeats (ISSR) ( Nyongesa et al, 2013 ; Abate et al, 2015 ), simple sequence repeats (SSRs) ( Uncu et al, 2015 ; Dossa et al, 2016 ; Sehr et al, 2016 ) amplified fragment length polymorphism (AFLP) ( Laurentin and Karlovsky, 2007 ); and Sequence Related Amplified Polymorphism (SRAP) (( Ali AL-somain et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity using biochemical, physiological, karyological and molecular markers of Sesamum indicum L

ALshamrani

Safhi²,

Alshaya³

et al. 2022

Front. Genet.

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The genetic diversity and the relationships among sesame cultivars were investigated using physiological and cyto/molecular analysis. To our information, no studies have yet been conducted on the genetic evaluation of sesame genotypes based on cyto/molecular analysis in Saudi Arabia. This study showed that genotype Bah-312 had the highest values from physiological and biochemical traits (plant height, harvest index, total plant dry matter, seed yield, oil content, and fatty acids content). Using 20 ISSR and 25 SCoT primers, the studied genotypes amplified 233 and 275 alleles, while the average polymorphism percentage (P%) was 65.32% (ISSR) and 77.8% (SCoT) across all the studied genotypes, respectively. To assess the markers efficiency analysis the polymorphism information contents (PIC), Marker Index (MI), Effective Multiplex Ratio (EMR), Resolving Power (Rp) were estimated. In general, primers (ISSR 2 & SCoT 21) and (ISSR 4 & SCoT 3) revealed the highest and lowest values for P %, PIC, MI, and EMR%. Furthermore, 188 positive and negative unique bands were detected, out of which ISSR generated 84, while 104 were amplified by SCoT analysis. In this regard, genotype Bah-312 generated 41 unique amplicons, and Jiz-511 genotype 23 unique amplicons. In the same context, the population genetics parameters, number of different alleles (Na), number of effective alleles (Ne), Shannon’s index (I), expected heterozygosity (He), and Unbiased Expected Heterozygosity (uHe), were calculated. ISSR marker showed the highest values for all the estimated parameters. In this regard, genotype Bah-312 exhibited the highest values (1.35, 1.37, 0.31, 0.21, 0.29) & (1.31, 1.35, 0.30, 0.20, 0.27) while, genotype Ahs-670 revealed the least values (1.29, 1.31, 0.26, 0.16, 0.23) &(1.14, 1.26, 0.22, 0.15, 0.20) for ISSR and SCoT markers respectively. For cytological data, according to the highest asymmetry index (AsK%) and lowest total form percentage (TF%) values, genotype Ahs-670 was the most advanced cultivar, and genotype Bah-312 was the most primitive one. According to the degree of asymmetry of karyotype (A) and intrachromosomal asymmetry index (A1), sesame genotype Ahs-670 was the most asymmetrical, and Bah-312 was the most symmetrical genotype. This study gives some helpful information about the genetic diversity of six sesame landraces. The variation harbored by these landraces could be used in sesame breeding programs.

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Assessment of Genetic Diversity in Ethiopian Sesame (Sesamum indicum L.) Germplasm Using ISSR Markers

Cited by 5 publications

References 20 publications

Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers

Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers

The Emerging Oilseed Crop Sesamum indicum Enters the “Omics” Era

Genetic diversity using biochemical, physiological, karyological and molecular markers of Sesamum indicum L

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