Assessment of genetic diversity among wheat somaclonal variants lines using morphological traits and molecular markers

Abouzied, Hanaa

doi:10.5897/ajb11.1759

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Therefore, these primers can be recommended for genetic evaluation of Azerbaijan wheat accessions. Khavarinejad and Karimov (2012) detected 33 and 17 polymorphic alleles for spring wheat genotypes by RAPD and SSR markers, respectively. Xgwm 120-2B had the most PIC with 57%.…”

Section: Resultsmentioning

confidence: 97%

Evaluation of Genetic Diversity Using Ssr Markers and Link With Drought Response of Azerbaijani Durum Wheat (Triticum Durum L.) Genotypes

Mammadova,

AMRI,

AKPAROV

et al. 2023

Preprint

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Genetic diversity of 46 azerbaijani durum wheat (triticum durum l.) Genotypes were screened using simple sequence repeats (ssrs). These accessions were collected from various bioclimatic regions of azerbaijan. Out of the used twenty-two primers, 13 primers showed polymorphism and were selected for the analyses. Among the genotypes under study, 31 alleles were detected. Highest number of alleles was detected in locus gwm 335 (on chromosome 5b) and on locus gwm 445 (on chromosome 2a) with 5 and 4 alleles, respectively. The lowest number of alleles was determined in locus gwm 617 with only 1 allele. For a, b and d genomes, the total number of alleles detected were 14, 15 and 2, respectively. Pic value between studied ssr markers was 0.912 and this result shows high genetic diversity between azerbaijani durum wheat genotypes. Therefore, these primers can be recommended for studying genetic diversity of azerbaijani durum wheat accessions. The genetic structure of the genotypes was analyzed and the upgma dendrogram revealed five major clusters using nei genetic distance index. The results revealed that ssr markers can efficiently evaluate genetic variation in the wheat samples. Based on the previous characterization of drought response of these genotypes, links could be established between the ssr markers and drought tolerance. If some of the ssr markers are confirmed for their association s with drought tolerance, then, they can be used as markers for identification of drought tolerant varieties needed to enhance wheat productivity for farmers dealing with harsh conditions.

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Section: Resultsmentioning

confidence: 97%

Evaluation of Genetic Diversity Using Ssr Markers and Link With Drought Response of Azerbaijani Durum Wheat (Triticum Durum L.) Genotypes

Mammadova,

AMRI,

AKPAROV

et al. 2023

Preprint

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“…Somaclonal variations can be detected easily by morphological, cytological, biochemical and molecular markers. Detection of genetic polymorphism based on random amplified polymorphic DNA (RAPD) has found successful application in describing somaclonal variability in rye [10], barley [11] and wheat [12]. Coupled restriction enzyme digestion-random amplification (CRED-RA) has been used for the identification of DNA methylation, which is a type of epigenetic variation.…”

Section: Introductionmentioning

confidence: 99%

Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)

Aydın

Arslan

Taspinar

et al. 2016

Biotechnology & Biotechnological Equipment

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Genetic variations, and especially somaclonal variations, are undesirable in genetic transformation. In this study, random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) markers were used for detection of the variation in calli that were obtained from endosperm-supported mature embryo of rye on Murashige and Skoog (MS) medium containing different auxins (2,4-D, dicamba and picloram) at a range of different concentrations (2, 4, 6, 8, 10 and 12 mg/L). High level of auxins caused a reduction in the genomic template stability (GTS) value. While the highest GTS was observed in the calli maintained on MS medium with 2 mg/L dicamba (98.4%), the calli maintained on MS medium with 12 mg/L picloram were found to show the least GTS (81.7%) when RAPD patterns were analysed. Epigenetic changes were more frequent and variable than genetic changes when compared to RAPD and CRED-RA results. DNA hypermethylation was observed at higher concentrations of 2,4-D and picloram, whereas DNA hypomethylation was observed in dicamba. These results indicate that RAPD and CRED-RA techniques can be used for detection of somaclonal variation in in vitro cultures, which is a fundamental step in plant genetic transformation.

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In Vitro Somaclonal Variation as Initial Material for Bread Wheat Breeding

Nikitina¹,

Khlebova²,

Пронина³

2015

abs

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СОМАКЛОНАЛЬНАЯ ИЗМЕНЧИВОСТЬ IN VITRO КАК ИСТОЧНИК СОЗДАНИЯ ИСХОДНОГО МАТЕРИАЛА ДЛЯ СЕЛЕКЦИИ МЯГКОЙ ПШЕНИЦЫ¹Алтайский научно-исследовательский институт сельского хозяйства, Барнаул, Россия, E-mail: aniizis@ab.ru ²Алтайский государственный университет, Барнаул, Россия E-mail: hlebova61@mail.ru The genotypes with a wide spectrum of variable features which could be of great interest for producing new forms were revealed. It is noted that the display of traits affected not only a genotype, but changes occurring at the cellular level during in vitro cultivation. Significant differences among somaclones derived from a callus were stated.

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Assessment of genetic diversity among wheat somaclonal variants lines using morphological traits and molecular markers

Cited by 5 publications

References 39 publications

Evaluation of Genetic Diversity Using Ssr Markers and Link With Drought Response of Azerbaijani Durum Wheat (Triticum Durum L.) Genotypes

Evaluation of Genetic Diversity Using Ssr Markers and Link With Drought Response of Azerbaijani Durum Wheat (Triticum Durum L.) Genotypes

Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)

In Vitro Somaclonal Variation as Initial Material for Bread Wheat Breeding

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