Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria

Shuaibu, Iliyasu Yunusa; Hadiza, J. A.; Yusha’u, M; Kabiru, M. Y.; Ahmad, Mani Malam; Garba, Lawal; Adamu, M.T.; Khairiyya, M

doi:10.1166/asl.2018.11457

Cited by 2 publications

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“…Moreover, it was generally observed that methanolic extract demonstrated more inhibitory effect against all the test bacteria compared to ethanolic extract. This could be attributed to higher polarity of methanol over the ethanol as the higher the polarity of an organic solvent the more its extractability of plant active compound which could be justified by the total extracts recovered following extraction as presented in failure globally [19][20][21][22].…”

Section: Minimum Inhibitory Concentrations and Minimum Bactericidal C...mentioning

confidence: 99%

Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria

Garba¹,

Muhammad²,

Adamu³

et al. 2021

J. Fundam and Appl Sci.

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Extended-spectrum β-lactamase (ESβL) enzymes are produced by certain bacteria as a mechanism of resistance to β-lactam and extended-spectrumβ-lactam antibiotics. Previous investigations have confirmed that M. oleifera contains several bioactive compounds. This study aimed at evaluating the in vitro antibacterial effects of ethanolic and methanolic leaf extracts of M. oleifera on extended-spectrum β-lactamase producing bacteria. The leaf extracts of the plant were prepared, screened for bioactive compounds and evaluated for in vitro inhibitory effects against the ESβL-producing bacteria using agar well diffusion method. Different phytochemicals were detected from the extracts. Both methanolic and ethanolic leaf extracts showed a high inhibitory activity, which increased with an increase in concentration, from lowest to highest based on the zones of growth inhibition produced. Amongst the test organisms, Klebsiella pneumoniae was the most sensitive isolate to methanolic extract at 200, 100, 50 and 25mg/mL followed by Escherichia coli, and then Proteus mirabilis. However, in terms of the ethanolic extract, using the same concentrations as those of methanol extracts, E. coli was found to be the most sensitive isolate followed by K. pneumoniae and then P. mirabilis.

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Section: Minimum Inhibitory Concentrations and Minimum Bactericidal C...mentioning

confidence: 99%

Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria

Garba¹,

Muhammad²,

Adamu³

et al. 2021

J. Fundam and Appl Sci.

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“…In developing countries like Nigeria, control over the processing and sales of hawked foods have not been achieved presumably because most of the vendors and sellers lack adequate knowledge of food processing and handling practices. As a result, extensive studies have been conducted on the nutritional and microbial quality of Kunun-zaki and other hawked indigenous drinks and beverages to increase public awareness, and draw the attention of the relevant food authorities to the health risks and hazards associated with the consumption of such contaminated food products [15,[31][32][33][34]. Following up from our previous study [15], in this study, we aimed to determine the presence of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA) and extended-spectrum beta-lactamase (ESBL) producing Salmonella spp in Kunun-zaki drinks sold in Umuahia metropolis, Nigeria.…”

Section: Methicillin-resistantmentioning

confidence: 99%

Screening of Kunun-zaki for Methicillin-Resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Staphylococcus aureus (VRSA) and Extended-Spectrum Beta-Lactamase (ESBL) Producing Salmonella spp.

Kelechi

Emeaso

Chukwuemeka

et al. 2020

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Kunun-zaki is an indigenous, fermented, non-alcoholic cereal-based beverage produced and consumed primarily in Northern Nigeria. Due to its high nutritional and moisture content, it provides an ideal environment for the growth of foodborne pathogens. This study determined the presence of Methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant Staphylococcus aureus (VRSA) and Extended spectrum beta lactamase (ESBL) producing Salmonella spp in Kunun-zaki sold in Umuahia metropolis, Nigeria. Out of the 40 Kunun-zaki samples examined, Salmonella spp was detected in 19 (47.5%), whereas S. aureus was detected in 22 (55%) of the samples. The total Salmonella count ranged from 1.90×105-9.70×106 (CFU/ml), whereas the total S. aureus count ranged from 3.40×104-7.50×106 (CFU/ml); these microbial counts do not conform to the standard limits of detectable microbes in ready-to-eat food samples as stipulated by the Center for Food Safety. All the isolates were subjected to antibiotic susceptibility testing using the modified Kirby Bauer disc diffusion method. On the one hand, most of the Salmonella spp were resistant to the beta-lactam class of antibiotics used (IMP 58%, MEM 90%, AMP 53%). On the other hand, the Salmonella isolates showed the highest sensitivities to ceftazidime (95%) and ofloxacin (90%). The S. aureus isolates revealed high susceptibility to ofloxacin (96%), and major resistance to oxacillin (41%) and the oxyimino-cephalosporin antibiotics used (CAZ 55%, CTR 41%). MRSA, VRSA and ESBL producing Enterobacteriaceae are amongst the most critical multidrug-resistant bacterial pathogens. In this study, 26% of the Salmonella spp isolates were confirmed to be ESBL producers, whereas 41% of the isolates were MRSA, 23% were VRSA, and 68% were Vancomycin intermediate Staphylococcus aureus (VISA). Hence, the Kunun-zaki could constitute a reservoir for the dissemination of multidrug-resistant foodborne pathogens if not correctly quality controlled.

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Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria

Cited by 2 publications

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Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria

Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria

Screening of Kunun-zaki for Methicillin-Resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Staphylococcus aureus (VRSA) and Extended-Spectrum Beta-Lactamase (ESBL) Producing Salmonella spp.

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