Assessment of Endothelial Dysfunction in Pregnant Women With Obesity and Preeclampsia

Zelinka-Khobzey, M. M.; Tarasenko, K. V.; Мамонтова, Тетяна Василівна

doi:10.36740/wlek202108122

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…We analyzed the concentration of each type of LEV separately, and we found that this group of pregnant women showed an increase in the levels of circulating endothelial LEVs expressing E-selectin (CD62E) and ICAM-1 (CD54) on their surface in the later stage of pregnancy, which reflects the activation of endothelial cells and endothelial dysfunction ( Jiménez et al, 2003 ; Zhang, 2022 ; Mezine et al, 2023 ). The levels of endothelium-derived LEVs observed in the present study were similar to those observed in women with PE in the third trimester of pregnancy ( Zelinka-Khobzey et al, 2021 ). In this sense, it has been shown that an increased content of circulating endothelial EVs in the blood of women with PE indicates endothelial damage, and the count of this type of EVs in the blood may serve as a marker for the severity of endothelial dysfunction in pregnant women ( Zelinka-Khobzey et al, 2021 ).…”

Section: Discussionsupporting

confidence: 87%

“…The levels of endothelium-derived LEVs observed in the present study were similar to those observed in women with PE in the third trimester of pregnancy ( Zelinka-Khobzey et al, 2021 ). In this sense, it has been shown that an increased content of circulating endothelial EVs in the blood of women with PE indicates endothelial damage, and the count of this type of EVs in the blood may serve as a marker for the severity of endothelial dysfunction in pregnant women ( Zelinka-Khobzey et al, 2021 ). Moreover, increased levels of endothelial LEVs in the group of women treated with the placebo were also accompanied by an elevation in the plasma concentration of LEVs derived from syncytiotrophoblasts, platelets, monocytes, and other leukocytes, at the end of pregnancy.…”

Section: Discussionsupporting

confidence: 87%

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Pravastatin reduces plasma levels of extracellular vesicles in pregnancies at high risk of term preeclampsia

et al. 2023

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Introduction: Elevated plasma levels of extracellular vesicles have been associated with impaired placentation, angiogenesis imbalance, intravascular inflammation, and endothelial dysfunction in women with preeclampsia, thus suggesting that circulating vesicles may be a good therapeutic target for the treatment of the disease. Recently, statins have been considered a potential treatment for the prevention of preeclampsia because of their pleiotropic effects, including the improvement of endothelial dysfunction and inhibition of inflammatory responses. However, the effects of these drugs on circulating vesicles concentration in women at risk of preeclampsia have not been established. Herein, we aimed to assess the effects of pravastatin on circulating extracellular vesicle generation in women at high risk of term preeclampsia.Methods: In a sample of 68 singleton pregnant women participating in the multicenter, double-blind, placebo-controlled STATIN trial (Nº EducraCT 2016-005206-19 ISRCTN), 35 women received a placebo and 33 women received a 20 mg/day dose of pravastatin for approximately 3 weeks (from 35 to 37 weeks of gestation until delivery). Large extracellular vesicles were characterized and quantified by flow cytometry using annexin V and cell-specific antibodies directed against platelet, endothelial, leukocyte, and syncytiotrophoblast cell surface markers.Results: In women who received the placebo, a significant increase in the plasma levels of large extracellular vesicles from platelets (34%, p < 0.01), leukocytes (33%, p < 0.01), monocytes (60%, p < 0.01), endothelial cells (40%, p < 0.05), and syncytiotrophoblast cells (22%, p < 0.05) were observed. However, treatment with pravastatin significantly reduced the plasma levels of large extracellular vesicles from platelets (42%, p < 0.001), leukocytes (25%, p < 0.001), monocytes (61%, p < 0.001), endothelial cells (69%, p < 0.001), activated endothelial cells (55%, p < 0.001), and syncytiotrophoblast cells (44%, p < 0.001).Discussion: These results indicate that pravastatin reduces the levels of activated cell-derived membrane vesicles from the maternal vasculature, blood, and placental syncytiotrophoblast of women at high risk of term preeclampsia, suggesting that this statin may be beneficial in reducing endothelial dysfunction and pro-inflammatory and pro-coagulatory state characteristics of the disease.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 87%

Pravastatin reduces plasma levels of extracellular vesicles in pregnancies at high risk of term preeclampsia

et al. 2023

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The Role of Obesity in the Development of Preeclampsia

Schiavone,

Pérez,

Aquieri

et al. 2024

Curr Hypertens Rep

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Morphometric Characteristics of Placenta in Women With Preeclampsia and Obesity Compared to Women With Normal Body Weight

Zelinka-Khobzey,

Tarasenko,

Nesterenko

et al. 2023

Wiad Lek

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The aim: to investigate the morphometric characteristics of placentas in women with comorbidity of preeclampsia and obesity compared to women with physiological body weight, and to assess the efficacy of the prophylactic therapy course developed to prevent the occurrence of preeclampsia in pregnant women with obesity. Materials and methods: 25 biopsy samples of placental tissue were taken from women between 37 and 40 weeks of gestation with a physiological body weight and with class II obesity. The women were divided into five groups of five women in each: the 1st group included women with physiological body weight without obstetric and somatic pathology; the 2nd group involved women with physiological body weight, whose pregnancy was complicated with preeclampsia; the 3rd group was made up of women with class II obesity whose pregnancy was complicated with preeclampsia; the 4th group consisted of women with class II obesity, who received the special prophylactic therapy course, and the 5th group included women with class II obesity, who did not receive the prophylactic therapy course. Results: The analysis of morphometric parameters of placenta samples taken from women with preeclampsia and obesity demonstrates a number of com¬pensatory and adaptive changes in placenta under hypoxic conditions, and the most important of them include a significant decrease in the number and the mean diameter of the terminal villi, the reduction of volume of villious tree, an increase in the diameter of the capillaries of terminal villi. The morphometric parameters and histological structure in placenta samples from women with obesity, who received the special the prophylactic therapy course, as well as in placenta samples of the control group were similar to the gestational normative values. Conclusions: The morphometric investigation of placenta samples taken from women with comorbidity of preeclampsia and obesity has shown a significant decrease in the mean diameter of the terminal villi and an increase in the diameter of the capillaries of these terminal villi when compared with a group of women with preeclampsia and physiological body weight. The study has also demonstrated the distortion of the percentage ratio of the volume of the intervil¬lous space and the ratio of medium-calibre villi. The combination of these changes indicates a lack of adaptive capabilities in the placenta during preeclampsia under increasing hypoxic condition.

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Assessment of Endothelial Dysfunction in Pregnant Women With Obesity and Preeclampsia

Cited by 3 publications

References 17 publications

Pravastatin reduces plasma levels of extracellular vesicles in pregnancies at high risk of term preeclampsia

Pravastatin reduces plasma levels of extracellular vesicles in pregnancies at high risk of term preeclampsia

The Role of Obesity in the Development of Preeclampsia

Morphometric Characteristics of Placenta in Women With Preeclampsia and Obesity Compared to Women With Normal Body Weight

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