Assessment of DNA degradation induced by thermal and UV radiation processing: Implications for quantification of genetically modified organisms

Ballari, Rajashekhar V.; Martin, Asha

doi:10.1016/j.foodchem.2013.05.032

Cited by 40 publications

(29 citation statements)

References 27 publications

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“…The various mechanisms that contribute to DNA degradation have attracted considerable attention in recent years (Ballari & Martin, 2013). Food processing and storage could cause DNA degradation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of milk quality during storage based on DNA extracted from milk

Liao

Gao

Ting

et al. 2018

CyTA - Journal of Food

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This study investigated the changes of DNA and milk quality during storage at temperatures of 2°C, 10°C and 20°C. Milk quality was assessed by measuring the milk protein, milk fat, lactose, pH and acidity. The quality of DNA extracted from milk was evaluated by monitoring DNA content, purity, and degradation as well as by amplifying target sequences from mitochondrial and nuclear genes. In addition, nonlinear regression models were used to analyze the correlations between DNA content and milk quality. As expected, the deterioration was observed in both DNA and milk quality during storage. Mathematical models showed the high correlations (R 2 > 0.9) between DNA content and milk quality. These established mathematical models hold the potential to evaluate the milk quality and may be applied practically in the process design and quality control of dairy products in the future.Valoración de la calidad de la leche durante su almacenamiento basada en el ADN extraído de la leche RESUMEN El presente estudio investigó los cambios que se producen en el ADN y la calidad de la leche durante su almacenamiento a temperaturas de 2°C, 10°C y 20°C. Al respecto, la calidad de la leche fue valorada a partir de la medición de la proteína láctea, la grasa láctea, la lactosa, el contenido de ADN, su pureza y su degradación, y de la amplificación de las secuencias objetivo de los genes mitocondriales y nucleares. Asimismo, se utilizaron modelos de regresión no lineales para analizar las correlaciones entre el contenido de ADN y la calidad de la leche. Como se esperaba, durante el almacenamiento se produjo el deterioro tanto del ADN como de la calidad de la leche. Los modelos matemáticos dieron cuenta de elevadas correlaciones (R 2 >0.9) entre el contenido de ADN y la calidad de la leche. Estos modelos matemáticos establecidos posibilitan evaluar la calidad de la leche y en el futuro podrán ser aplicados de manera práctica a la hora de diseñar su procesamiento y realizar el control de calidad de productos lácteos. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Assessment of milk quality during storage based on DNA extracted from milk

Liao

Gao

Ting

et al. 2018

CyTA - Journal of Food

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show abstract

“…The other face, the DNA damage caused by physical or chemical agents, such as oxidation, alkylation, ultrasound, UV radiation and other, were widely studied (Yakovchuk et al, 2006;Grokhovsky et al, 2011;Ballari and Martin, 2013). There were distinct cleavage mechanisms for different damage, as DNA degradation at high temperature was thought to be broke the covalent bonds within DNA strand (Moshe et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Degradation and detection of transgenic Bacillus thuringiensis DNA and proteins in flour of three genetically modified rice events submitted to a set of thermal processes

Wang

Chen

Dai

et al. 2015

Food and Chemical Toxicology

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“…9 These strains of CRE can cause devastating infection with high fatality rates, typically in people who are debilitated, ill, or subjected to invasive medical procedures. 10 These infections result in an estimated 600 deaths per year in the US. 2 While this number is a small fraction of all AMR deaths (the most deaths are due to methicillinresistant Staphylococcus aureus and drug-resistant pneumococcus), these nearly pan-resistant CRE infections were unheard of just a few years ago and are spreading.…”

Section: An International Health Prioritymentioning

confidence: 99%