Assessment of DNA Damage in Leukocytes of Patients With Coronary Artery Disease by Comet Assay

Abstract

SummaryDNA damage in the peripheral blood leukocytes (PBL) of patients with coronary artery disease (CAD) was investigated using the sensitive alkaline single cell gel electrophoresis (SCGE)/comet assay.This case-control study consisted of CAD patients (n = 200; mean age, 59.04 ± 0.75 years) undergoing treatment at local hospitals and age-, sex-, and ethnicity-matched healthy controls (n = 200; mean age, 57.88 ± 0.96 years) from the general population.CAD patients had significantly (P < 0.001) increased DNA damage (tail DNA percent (T-DNA %) 22.45 ± 0.50 versus 5.81 ± 0.28; tail moment (TM) 89.35 ± 3.16 versus 9.98 ± 0.69; Olive tail moment (OTM) 60.50 ± 1.79 versus 10.94 ± 0.63; damage frequency (DF) 91.12 ± 0.93 versus 41.78 ± 2.04, damage index (DI) 173.68 ± 3.36 versus 48.53 ± 2.59) compared to controls. Patients with acute myocardial infarction (AMI) showed significantly higher DNA damage than patients with unstable angina (UA) (T-DNA % 24.05 ± 0.87 versus 21.06 ± 0.90; TM 100.02 ± 6.19 versus 81.61 ± 5.84; OTM 66.19 ± 3.20 versus 56.47 ± 3.33; DF 94.02 ± 0.84 versus 91.10 ± 1.16, DI 184.13 ± 5.33 versus 166.42 ± 5.89). Moreover, DNA damage was found to be significantly (P < 0.05) elevated in patients receiving ecosprin, ramipril, and metoprolol therapy compared to aspirin and nitrocontin.The increased DNA damage in CAD patients may be the consequence of disease and/or drug therapy. These observations are of concern because unrepaired DNA can lead to malignancy, and the likelihood of increasing mortality and morbidity rates in CAD patients. (Int Heart J 2017; 58: 271-274)

Analysis of Genotoxic Damage in Different Diseases

“…According to their studies, DNA damage was significantly higher in coronary artery disease patients than in the control group (p<0.001). Similarly, Bhat & Gandhi (2017) showed that DNA damage in cardiovascular patients was significantly (p<0.001) more than in the control group. They evaluated comet parameters and found that tail DNA percent was 22.45 ± 0.50 and tail moment was 89.35±3.16.…”

Image Mosaic Algorithm-Based Analysis of Effects of Low-Dose X-Rays on Blood Immune Cells

“…In this study, the neutral single-cell gel electrophoresis method was used for detection, which mainly detected the breaking of DNA double strands, with high specificity. Compared with other parameters, and TM and TDNA had a higher correlation with the degree of DNA damage and were more sensitive and accurate [17,18]. According to the research of Chen et al [19], although many mosaic algorithms are implemented based on SIFT algorithm, radiation damage suffered by cardiovascular intervention patients is generally acute radiation damage.…”