Assessment of DNA damage in Brazilian workers occupationally exposed to pesticides: a study from Central Brazil

Khayat, Carolinne Borges; Costa, Emília Oliveira Alves; Gonçalves, Macks Wendhell; Cunha, Damiana Mirian da Cruz e; Cruz, Alex Silva da; Melo, Caroline Oliveira de Araújo; Cruz, Aparecido Divino da; Silva, Daniela de Melo e

doi:10.1007/s11356-013-1747-1

Cited by 33 publications

(21 citation statements)

References 28 publications

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“…So, the occupational exposure increases cytogenetic damage induced by pesticides exposure. Researches on pesticide-exposed workers from all over the world in the last decade also demonstrated statistically signifi cant DNA damage, MN and CAs [17][18][19][20][21][22][23][24][25][26][27][28][29][30]. In a recent study, it was found an increased frequency of MN and CAs in pesticide-exposed agricultural workers from northeastern Brazil [25].…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Aberrations in agricultural farmers exposed to pesticides

Demırhan¹,

Akbaba²,

Çelik³

et al. 2019

Adv Toxicol Toxic Effects

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Pesticides which constitute an group of environmental pollutants are commonly used in agriculture to protect crops. Pesticide exposure may be associated with increased risk of genotoxicity and carcinogenesis. Therefore, they pose a potential risk to farmers and environment. The aim of the present study is to investigate whether occupational exposure to various pesticides causes Chromosomal Aberrations (CAs) in agricultural farmers. The frequency of CAs in peripheral blood lymphocytes were evaluated in 35 Agricultural Farmers (AFs) and 40 control subjects living in Çukurova Region. The results showed that farmers exposed to pesticides had signifi cantly increased frequencies of CAs when compared with controls (P<0.05). Ageadjusted group comparisons showed that the frequency of CAs in the pesticide exposed AFs were 2,3 times higher than in the control group (OR: 2,3, 95% wald confi dence limits: 1,9-2,9). The confounding factors such as variable duration of pesticide exposure, age, smoking, alcohol consumption had no signifi cant effect on cytogenetic damage (P>0.05). Fragile sites (FSs) on the 1(q21-24), 1(q31-32), 2(q31-34), 2(q21-23), 3(p21), 3(p25) and 5(q31-q34) chromosomal regions were signifi cantly overexpressed in AFs, when compared to the control group (in 12.6% versus 4.3% of cells) (P<0.05). Our fi ndings indicate that occupational exposure to pesticides could cause cytogenetic damage in somatic cells of AFs. It is known that accumulation of CAs is a crucial step for initiation of many cancers. Therefore, it is suggested that the exposed workers should be warned about the potential harmful effects of pesticides and relevant authorities should safeguard that protective measures are taken by farmers while working in agricultural fi elds.

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Section: Discussionmentioning

confidence: 99%

Chromosomal Aberrations in agricultural farmers exposed to pesticides

Demırhan¹,

Akbaba²,

Çelik³

et al. 2019

Adv Toxicol Toxic Effects

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“…Recent guidance for comet assay methodology suggests that the most useful comet measurement is the percentage of DNA in the comet tail ( Anderson et al 2013 , Azqueta and Collins 2013 , Collins et al 2014 ). Only one of the six comet studies reported measurement of percentage of DNA in the comet tail ( Khayat et al 2013 ).…”

Section: General Methodologymentioning

confidence: 99%

Review of genotoxicity biomonitoring studies of glyphosate-based formulations

Kier

2015

Critical Reviews in Toxicology

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Human and environmental genotoxicity biomonitoring studies involving exposure to glyphosate-based formulations (GBFs) were reviewed to complement an earlier review of experimental genotoxicity studies of glyphosate and GBFs. The environmental and most of the human biomonitoring studies were not informative because there was either a very low frequency of GBF exposure or exposure to a large number of pesticides without analysis of specific pesticide effects. One pesticide sprayer biomonitoring study indicated there was not a statistically significant relationship between frequency of GBF exposure reported for the last spraying season and oxidative DNA damage. There were three studies of human populations in regions of GBF aerial spraying. One study found increases for the cytokinesis-block micronucleus endpoint but these increases did not show statistically significant associations with self-reported spray exposure and were not consistent with application rates. A second study found increases for the blood cell comet endpoint at high exposures causing toxicity. However, a follow-up to this study 2 years after spraying did not indicate chromosomal effects. The results of the biomonitoring studies do not contradict an earlier conclusion derived from experimental genotoxicity studies that typical GBFs do not appear to present significant genotoxic risk under normal conditions of human or environmental exposures.

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“…However, indiscriminate use contaminates many environmental components including soil, water, air, and kills other nontargeted species. [20][21][22][23] Pesticide toxicity is of two types-acute and chronic. Among the chronic effects, severe hematotoxicity followed by BM failure and development of AA is a major health hazard in countries where a large population is economically dependent on agriculture such as India and Thailand.…”

Section: Introductionmentioning

confidence: 99%

Morphogen signaling by Wnt/β‐catenin pathway and microenvironmental alteration in the bone marrow of agricultural pesticide exposure‐induced experimental aplastic anemia

Chattopadhyay¹,

Law²

2020

J Biochem & Molecular Tox

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The etiologic link between pesticide toxicity and aplastic anemia in agricultural and agro‐industrial setting has been frequently reported in epidemiological studies conducted worldwide. Chronic pesticide toxicity causes long‐term bone marrow injury and perturbs the normal hematopoietic physiology, including survival of hematopoietic progenitor cells and bone marrow's blood cell forming ability. The purpose of this study is to understand the mechanism of pesticide toxicity‐mediated bone marrow aplasia by studying Wnt/β‐catenin signaling pathway and microenvironmental stromal components. An agricultural pesticide formulation comprising of cypermethrin, chlorpyriphos, and hexaconazole was used to induce bone marrow aplasia in inbred Swiss albino mice. Marrow failure followed by the onset of aplastic condition was confirmed by pancytopenic peripheral blood and hypocellular bone marrow filled with adipocytes. Significant downregulation of canonical Wnt/β‐catenin signaling was identified by expression analysis of Wnt3a, β‐catenin, and telomerase reverse transcriptase in the aplastic bone marrow hematopoietic stem/progenitor compartment. Along with signaling deregulation, disruption in both the osteoblastic and vascular stromal components was observed in the pesticide‐exposed bone marrow microenvironment when compared to control. In this study, we tried to establish the correlation among disease pathophysiology, signaling deregulation in the hematopoietic cells, and bone marrow microenvironmental alteration during environmental exposure‐mediated aplastic hematopoietic catastrophe, which may shed light on the unexplored mechanistic perspective of this fatal blood disease.

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Assessment of DNA damage in Brazilian workers occupationally exposed to pesticides: a study from Central Brazil

Cited by 33 publications

References 28 publications

Chromosomal Aberrations in agricultural farmers exposed to pesticides

Chromosomal Aberrations in agricultural farmers exposed to pesticides

Review of genotoxicity biomonitoring studies of glyphosate-based formulations

Morphogen signaling by Wnt/β‐catenin pathway and microenvironmental alteration in the bone marrow of agricultural pesticide exposure‐induced experimental aplastic anemia

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