Assessment of cell surface properties and adhesion potential of selected probiotic strains

Abstract

Aim: To evaluate the physicochemical cell surface and adhesive properties of selected probiotic strains for human use. Methods and Results: Probiotic strains, Bifidobacterium longum B6, Lactobacillus acidophilus ADH, Lactobacillus paracasei, Lactobacillus rhamnosus GG, Lactobacillus brevis, Lactobacillus casei, Leuconostoc mesenteroides and Pediococcus acidilactici were tested for the physicochemical properties of cell surfaces and the adhesion abilities against foodborne pathogens. Bif. longum B6 (53·6%) and Lact. rhamnosus GG (46·5%) showed the highest hydrophobicity, while the least affinity to xylene was observed in Ped. acidilactici (10·4%). Bifidobacterium longum B6 showed the strongest coaggregation phenotype with Listeria monocytogenes (53·0%), Shigella boydii (42·0%) and Staphylococcus aureus (45·9%). Lactobacillus rhamnosus GG had the strong binding ability to Caco‐2 cells and effectively inhibited the adhesion of L. monocytogenes, Salmonella Typhimurium, Sh. boydii and Staph. aureus to Caco‐2 cells. The hydrophobicity was highly correlated with coaggregative abilities and competitive inhibition, suggesting a good relationship between in vitro adhesion and in vivo colonization. Conclusion: The results suggest that Bif. longum B6 and Lact. rhamnosus GG can be candidate probiotics available for human consumption. Significance and Impact of the Study: Because the use of probiotic strains has been more concerned with their beneficial effects in the GI tract, it is essential to examine the potential of probiotic strains based on the physicochemical properties in terms of bacterial‐binding and adhesion capabilities.

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“…Lactobacilli with high autoaggregation ability showed high hydrophobicity (Chen et al, 2010;Nikolic et al, 2010). Previous studies indicated a correlation between adhesion ability and hydrophobicity of some lactobacilli (Wadstroum et al, 1987;Xu et al, 2009). Mainly cell surface-associated proteins are involved in aggregation and adhesion although many other factors existed like teichoic, lipoteichoic acids, and polysaccharide (Goh and Klaenhammer, 2010).…”

A Lactobacillus rhamnosus strain induces protection in different sites after Salmonella enterica subsp. enterica serovar Typhimurium challenge in gnotobiotic and conventional mice

“…This protection observed in treated gnotobiotic mice can be due to the reduced translocation of S. Typhumurium as proposed by other probiotics (Martins et al, 2007;Martins et al, 2010;Salva et al, 2010). This fact can be explained, at least in part, by the inhibition of both cell association and cell entry of this enterobacterium as described with other lactobacilli strains in enterocyte-like Caco-2 cells (Hudalt et al, 1997;Xu et al, 2009). With this in mind, Miyauchi et al (2009) demonstrated that the L. rhamnosus OLL2838 is effective for the suppression of the tumor necrosis factor (TNF)-α-induced barrier impairment in Caco-2 cells and dextran sodium sulfate-induced colitis in mice.…”

Safety and Antioxidant Properties of Five Probiotic <i>Lactobacillus plantarum</i> Strains Isolated from the Digestive Tract of Honey Bees

“…Auto-aggregation was significantly and positively correlated to hydrophobicity on n-hexane in our study. Correlation between hydrophobicity and adhesion ability of several lactobacilli was previously indicated in the studies [42,43]. Mostly cell surface-associated proteins are involved in aggregation and adhesion even though numerous additional factors existed like polysaccharide, lipoteichoic and teichoic acids [44].…”