Assessment of bone ingrowth into porous biomaterials using MICRO-CT

Jones, Anthony; Arns, Christoph H.; Sheppard, Adrian; Hutmacher, Dietmar W.; Milthorpe, Bruce; Knackstedt, Mark

doi:10.1016/j.biomaterials.2007.01.046

Cited by 377 publications

(275 citation statements)

References 38 publications

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“…According to specific study objectives, a customized strategy will be required to extract the necessary information in a robust way, ranging from (1) accurate and detailed (100s of mm scale) identification and quantification of different TE construct components limited to small TE construct samples and using phase-contrast imaging, 25 to (2) using standard desktop micro-or nano-CT as a routine 3D imaging technique for whole TE construct analysis (mm to cm scale) and quantification of mineralization after in vivo implantation. 10,13 The latter approach, without the use of a contrast agent, has also been used for static or bioreactor in vitro cultures, 16,18,19 showing distinct contrast differences between the mineralized matrix and scaffold. Phase-contrast imaging [24][25][26] or micro-CT combined with osmium tetroxide as a contrast agent 28 has shown its potential to assess nonmineralized ECM in an in vitro engineered TE construct.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, Xray microfocus computed tomography (micro-CT) has been frequently applied as a 3D quantitative imaging technique to assess the scaffold structure, [6][7][8] as well as bone ingrowth after in vivo implantation. 6,[9][10][11][12][13][14] Furthermore, it has been employed for time-lapsed follow-up of mineralization inside scaffolds during in vitro static [15][16][17] or bioreactor cultures. 13,18,19 In most of these studies, polymeric, ceramic, collagen scaffolds, or composites were used, in which, the mineralized extracellular matrix (ECM) could be separated from the scaffold for the purpose of volume calculations and no significant material-dependent artifacts were present.…”

Section: Introductionmentioning

confidence: 99%

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Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Papantoniou

Sonnaert

Geris

et al. 2014

Tissue Engineering Part C: Methods

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To successfully implement tissue-engineered (TE) constructs as part of a clinical therapy, it is necessary to develop quality control tools that will ensure accurate and consistent TE construct release specifications. Hence, advanced methods to monitor TE construct properties need to be further developed. In this study, we showed proof of concept for contrast-enhanced nanofocus computed tomography (CE-nano-CT) as a whole-construct imaging technique with a noninvasive potential that enables three-dimensional (3D) visualization and quantification of in vitro engineered extracellular matrix (ECM) in TE constructs. In particular, we performed a 3D qualitative and quantitative structural and spatial assessment of the in vitro engineered ECM, formed during static and perfusion bioreactor cell culture in 3D TE scaffolds, using two contrast agents, namely, Hexabrix Ò and phosphotungstic acid (PTA). To evaluate the potential of CE-nano-CT, a comparison was made to standardly used techniques such as Live/Dead viability/cytotoxicity, Picrosirius Red staining, and to net dry weight measurements of the TE constructs. When using Hexabrix as the contrast agent, the ECM volume fitted linearly with the net dry ECM weight independent from the flow rate used, thus suggesting that it stains most of the ECM. When using PTA as the contrast agent, comparing to net weight measurements showed that PTA only stains a part of the ECM. This was attributed to the binding specificity of this contrast agent. In addition, the PTA-stained CE-nano-CT data showed pronounced distinction between flow conditions when compared to Hexabrix, indicating culture-specific structural ECM differences. This novel type of information can contribute to optimize bioreactor culture conditions and potentially critical quality characteristics of TE constructs such as ECM quantity and homogeneity, facilitating the gradual transformation of TE constructs in well-characterized TE products.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Papantoniou

Sonnaert

Geris

et al. 2014

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

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“…Once individual events of interest were identified from the sequence, the data set was segmented into two phases (where CO 2 was treated as one phase and brine and rock were treated together as the single other phase) using the greyscale gradient as an input to a watershed algorithm, seeded using a 2D histogram (Jones et al 2007). The image-processing workflow is shown in Fig.…”

Section: Partially Saturated Imagesmentioning

confidence: 99%

The Imaging of Dynamic Multiphase Fluid Flow Using Synchrotron-Based X-ray Microtomography at Reservoir Conditions

et al. 2015

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Fast synchrotron-based X-ray microtomography was used to image the injection of super-critical CO 2 under subsurface conditions into a brine-saturated carbonate sample at the pore-scale with a voxel size of 3.64 µm and a temporal resolution of 45 s. Capillary pressure was measured from the images by finding the curvature of terminal menisci of both connected and disconnected CO 2 clusters. We provide an analysis of three individual dynamic drainage events at elevated temperatures and pressures on the tens of seconds timescale, showing nonlocal interface recession due to capillary pressure change, and both local and distal (non-local) snap-off. The measured capillary pressure change is not sufficient to explain snap-off in this system, as the disconnected CO 2 has a much lower capillary pressure than the connected CO 2 both before and after the event. Disconnected regions instead preserve extremely low dynamic capillary pressures generated during the event. Snap-off due to these dynamic effects is not only controlled by the pore topography and throat radius, but also by the local fluid arrangement. Whereas disconnected fluid configurations produced by local snap-off were rapidly reconnected with the connected CO 2 region, distal snap-off produced much more long-lasting fluid configurations, showing that dynamic forces can have a persistent impact on the pattern and sequence of drainage events.

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“…Um tamanho mínimo de poro entre 100 e 150 μm foi inicialmente estabelecida como o critério mais importante para um crescimento continuado do osso no interior dos poros, devido ao tamanho das célu-las, necessidades de migração e transporte, mas isso ainda é controverso [7,8] . Klawitter et al e Simslke et al estabeleceram um tamanho mínimo de poros de aproximadamente 100 μm para o crescimento ósseo dentro da estruturas porosas de cerâ-micas, com a presença de poros interconectados para permitir o crescimento interno das células, a vascularização e a difusão de nutrientes [9] .…”

Section: Resultsunclassified

Desenvolvimento e caracterização de suportes porosos de polietileno de ultra alto peso molecular (PEUAPM) para utilização como biomaterial para reposição e regeneração óssea

Aparecida

Guastaldi

Fook³

2008

Polímeros

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materiais sintéticos utilizados para este fim incluem metais, polímeros e cerâmicas e também a combinação destes nos chamados compósitos [1,2] .Grande atenção tem sido dada ao desenvolvimento de biomateriais porosos, incluindo a produção de membranas, recobrimentos e implantes [3] . A presença de poros na superfície de implantes proporciona sua fixação com o tecido vivo por crescimento de tecido através dos poros por todo o implante. Esta ligação tecido/implante poroso é denomina- IntroduçãoOs biomateriais podem ser considerados como produtos aptos para serem utilizados nos seres humanos com a finalidade de tratamento ou alívio de uma enfermidade ou lesão, e também para a substituição e modificação de sua anatomia ou de um processo fisiológico. Por isso, incluem qualquer produto natural, sintético e natural modificado que pode ser utilizado como um dispositivo médico ou parte dele. Os Desenvolvimento e Caracterização de Suportes Porosos de Polietileno de Ultra Alto Peso Molecular (PEUAPM) para Utilização como Biomaterial para Reposição e Regeneração ÓsseaAnahi H. Aparecida, Antonio C. Guastaldi Instituto de Química, UNESP Marcus V. L. Fook Departamento de Engenharia de Materiais, UFCGResumo: O uso de polímeros como biomateriais tem crescido nos últimos anos, principalmente como suportes poliméricos para regeneração tridimensional e substituição de tecidos. Embora o polietileno de ultra alto peso molecular (PEUAPM) apresente vantagens na sua utilização como biomaterial, principalmente como implante ortopédico, sua baixa reatividade química constitui-se como fator limitante para sua interação com o tecido ósseo. Neste contexto, a modificação morfológica deste polímero, tornando-o um material poroso, e sua associação com um material bioativo pode proporcionar a obtenção de um biomaterial adequado para a regeneração e reposição do tecido ósseo. Neste trabalho, foram preparados e caracterizados suportes porosos de PEUAPM, pela combinação das técnicas de lixiviação de sal e moldagem por compressão com a aplicação de diferentes pressões de compactação, visando a sua utilização como biomaterial para reposição e regeneração óssea. Os suportes poliméricos apresentaram porosidade interconectada com diâmetro médio de poros entre 34 e 49 μm e porosidade entre 39 e 53%, podendo ser classificados adequados para a utilização como biomaterial poroso. Palavras-chave: Biomateriais, polietileno de ultra alto peso molecular (PEUAPM), biomateriais porosos, lixiviação de sal, moldagem por compressão. Development of Ultra High Molecular Weight Polyethylene (UHMWPE) Porous Supports for Use as Biomaterial in Osseous Replacement and RegenerationAbstract: The use of polymer as biomaterials has increased in recent years, mainly as polymeric supports in the threedimensional regeneration and substitution of tissues. Although the ultra high molecular weight polyethylene (UHMWPE) shows advantages in its use as biomaterial, particularly as orthopedic implants, its low chemical reactivity constitutes a limiting factor for interaction with the oss...

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Assessment of bone ingrowth into porous biomaterials using MICRO-CT

Cited by 377 publications

References 38 publications

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

The Imaging of Dynamic Multiphase Fluid Flow Using Synchrotron-Based X-ray Microtomography at Reservoir Conditions

Desenvolvimento e caracterização de suportes porosos de polietileno de ultra alto peso molecular (PEUAPM) para utilização como biomaterial para reposição e regeneração óssea

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