Assessment of biological leaf tissue using biospeckle laser imaging technique

Abstract

We report on the application of an optical imaging technique, the biospeckle laser, as a potential tool to assess biological and medicinal plant leaves. The biospeckle laser technique is a non-invasive and non-destructive optical technique used to investigate biological objects. Just after their removal from plants, the torn leaves were used for biospeckle laser imaging. Quantitative evaluation of the biospeckle data using the inertia moment (IM) of the time history speckle pattern, showed that the IM can be utilized to provide a biospeckle signature to the plant leaves. It showed that leaves from different plants can have their own characteristic IM values. We further investigated the infected regions of the leaves that display a relatively lower biospeckle activity than the healthy tissue. It was easy to discriminate between the infected and healthy regions of the leaf tissue. The biospeckle technique can successfully be implemented as a potential tool for the taxonomy of quality leaves. Furthermore, the technique can help boost the quality of ayurvedic medicines.

Monitoring the apical growth characteristics of hairy roots using non‐invasive laser speckle contrast imaging

“…The infected tissue could be detected at an early stage from a decrease in the flux value, even before fungal contamination was visible (data not shown). Similar results were obtained by Ansari et al., 2018 [ 48 ] using laser speckle contrast analysis on infection sites on plant leaves, or in studies by Braga et al., 2005 [ 57 ] on the detection of fungi on bean seeds. Basically, the laser speckle monitoring method is not object‐specific and is characterized by a simple basic setup, which is why it can be transferred to a wide range of further biotechnological applications [ 36 , 43 , 46 ].…”

“…Speckle analyses of plant structures published by Ansari et al, 2018 [48], detected areas of infection on leaves of Plumeria rubra and Epipremnum aureum from divergent speckle fluctuations. Braga et al, 2009 [43] developed a simple system to determine biospeckle activity among in vitro root strands of Coffee arabica and Eucalyptus grandis shoot cultures in a solid culture medium.…”

“…Speckle measurements have also been used to study other biological processes and phenomena, such as assessing the quality of fruits [44,[53][54][55][56], detecting the growth of fungi, bacteria, and parasites, studying the viability of plant seeds [57,58], determining infections on leaves [48], and characterizing leaf and root growth [43,50].…”

Self‐Healing Soft Sensors: From Material Design to Implementation

“…Besides, Mujeeb et al. [ 145 ] reported another sophisticated method for visualizing the self‐healing process with lasers, which is based on high scattering properties of materials next to the damaged area. In another example, fluorescence properties were used to identify cracked and healed regions.…”

Investigation of the ripeness of oil palm fresh fruit bunches using bio-speckle imaging

“…This method is based on variations of laser light intensity or contrast dissipated by biological samples. Fruit is a complex element consisting of molecules of fruit tissue built of various sizes depending on the level of maturity and ripeness, These molecular sizes will affect the optical properties of the fruit associated with the skin's ability and the fruit tissue to absorb and scatter light so that it can be used to monitor fruit maturity levels [4].…”