The research highlights the issue of optimization of the membrane reverse osmosis separation for aqueous media characterized by high content of hardness salts. The pilot two-stage reverse osmosis plant was applied for the research. To improve the process of membrane reverse osmosis separation, antiscalants Avista Vitec 3000, Clarofos 381 and sodium tripolyphosphate were used. Waste water from a solid waste landfill, characterized by increased hardness, was used as the studied aqueous solution. The effectiveness of antiscalants impact on the process of membrane reverse osmosis separation was assessed basing on the membrane specific performance. The studies have shown that to select the right antiscalant, it is necessary to take into account the characteristics of the fed water composition, the operating mode of the membrane equipment and special requirements of the treatment technology.