Assessment of antimicrobial microspheres as a prospective novel treatment targeted towards the repair of perianal fistulae

Abstract

SUMMARY BackgroundNone of the proposed materials tested for the management of perianal fistulae has proven to be a definitive treatment.

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable Thermally Induced Phase Separation Microspheres

“…29,31,40 Relative to solid microcarriers, TIPS microcarriers of an equal size contain approximately 80-90% less polymer material. 40 This is advantageous as less degradation products are produced as the microcarriers degrade to be replaced by host tissue.…”

“…We have previously developed a novel type of biodegradable polymer microcarrier intended for minimally invasive treatment of incontinence and sphinctersparing treatment of fistulas that can be used for large-scale expansion of cells. [29][30][31][32] The microcarriers, composed of a biocompatible and degradable material, poly(D,L-lactideco-glycolide) (PLGA), are prepared using a thermally induced phase separation (TIPS) technique, resulting in a highly porous structure that facilitates controlled degradation compared with solid microcarriers. We have demonstrated the manufacture of TIPS microcarriers to be a scalable process that can be performed under GMP conditions, providing a clinically ready product.…”

Perianal fistulae in Crohnʼs Disease: Current and future approaches to treatment

“…Blaker et al 143 recently formulated porous poly(D,L-lactide-co-glycolide) (PLGA) microspheres containing either antibacterial silver-releasing degradable phosphate glass or metronidazole. Both types of microspheres were found to inhibit bacterial growth and thus may be suitable for use in perianal fistulae.…”

Alimentary Tract

“…To address this, collagen anal fistula plugs have been devised for the treatment of fistulas. A possible solution to this problem is the use of scaffold materials that provide both optimal conditions for rapid cell infiltration when implanted into tissue cavities and mechanical strength to maintain an open scaffold structure (Blaker et al, 2008). Reports of the plugs failing due to dislodgment from the tracts indicate this approach may not provide an ideal scaffold material to promote guided tissue regeneration and closure of the tract (Adamina et al, 2010).…”