Assessment of an ELISA method to support surveillance of bovine tuberculosis in Albania

Koni, Anita; Juma, A.; Morini, Matteo; Nardelli, S.; Connor, Robert; Koleci, Xhelil

doi:10.1186/s13620-016-0069-2

Cited by 12 publications

(16 citation statements)

References 13 publications

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“…On the other hand, the parallel strategy CCT + ELISA, would result in an increase on the proportion of herds infected by 60% and at animal level the increase of positive animals by 5.5%, these results are compatible with previous studies (17,25,36). Thus, the parallel interpretation of the diagnostic techniques that detect cellular and humoral immune response although can be recommended as supplemental test to detect false negative TST animals (17,25,33,34,36) should be considered according the local circumstances.…”

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosissupporting

confidence: 82%

“…On the other hand, in this study six animals' (1.50%) reactors on the SCT test were positive on ELISA IDEXX™, which differ from another study where no agreement was found between SCT testing and ELISA IDEXX™ (33). The lack of or low agreement between the positive results of the two tests may re ect different elements of the immune response (humoral and cell-mediated immunity) (33,34). The detection of CMI response to infection with M. bovis, as assessed by the TST usually fail to detect chronic stages of the infection (26,29,35).…”

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosiscontrasting

confidence: 53%

“…No overlap was found between the 22 animals with positive ELISA IDEXX™ and the two animals with positive CCT test results agreeing with similar ndings of other studies (33,34). On the other hand, in this study six animals' (1.50%) reactors on the SCT test were positive on ELISA IDEXX™, which differ from another study where no agreement was found between SCT testing and ELISA IDEXX™ (33). The lack of or low agreement between the positive results of the two tests may re ect different elements of the immune response (humoral and cell-mediated immunity) (33,34).…”

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosismentioning

confidence: 91%

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Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Carneiro

Sousa

Viana

et al. 2020

Preprint

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Background With a worldwide occurrence, bovine tuberculosis (bTB) is a zoonotic disease that is difficult to control, mainly due to the lack of a diagnostic testing to detect infected animals at all stages. Furthermore, the current standard diagnostic test, the Tuberculin Skin Test (TST), is logistically difficult and time consuming. To address this challenge, the aim of this study was to evaluate the sensitivity and specificity of a multiplex enzyme-linked immunosorbent assay (ELISA) comparing with the TST used for the diagnosis of tuberculosis in cattle in Brazil. The study included 400 Nelore females raised for beef on five farms, in different municipalities in Brazil. The comparative cervical test (CCT) was done and on the day of inoculation of the Purified Protein Derivative (PPD) blood samples were obtained and stored for further analysis of the ELISA IDEXX™ Mycobacterium bovis immunoassay. Results Lack of agreement between CCT and ELISA IDEXX™ was observed. No diagnosis described as positive reactor on the CCT was positive at the ELISA, indicating two false positive reactors and 22 negative reactors by CCT were positive by the ELISA IDEXX™. The ELISA IDEXX™ showed sensitivity significantly higher than the official CCT and no significant differences in specificity was observed. ELISA also detected infected animals and herds undetected by the CCT. The parallel use of CCT and ELISA increased sensitivity and the feasibility bTB screening, thus improving the cleaning of the herds. Conclusions The results obtained here suggest that the ELISA IDEXX™ may be a supplemental test for the detection of Mycobacterium bovis infection in regions without routine testing and slaughter, where the disease generally progresses to more advanced stages and antibody responses are likely to be more prevalent. The results provided evidence to support the validation of the ELISA IDEXX™ as a supplemental test for bTB eradication programs.

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Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosissupporting

confidence: 82%

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosiscontrasting

confidence: 53%

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosismentioning

confidence: 91%

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Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Carneiro

Sousa

Viana

et al. 2020

Preprint

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“…Traditional culture and PM examination methods are the current procedures for TB detection and control. Several studies on camel TB have been conducted in several countries, including Egypt, confirming the occurrence of TB in camel populations [19,20].…”

Section: Discussionmentioning

confidence: 89%

Seroprevalence and molecular characterization of Mycobacterium bovis infection in camels (Camelus dromedarius) in the Delta region, Egypt

et al. 2019

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Aim: This study aimed to determine the prevalence rates of Mycobacterium infection in camel sera collected before slaughter and gross lesion tissue collected at postmortem (PM) using enzyme-linked immunosorbent assay (ELISA), bacteriological culture, and polymerase chain reaction (PCR). In addition, serum samples from humans who had occupational contact with camels were tested by ELISA and sputum sample by culture. Materials and Methods: ELISA was performed on serum samples antemortem. In addition, bacteriological culture and PCR were conducted after PM. Tuberculosis infection was identified in humans who had contact with camels using ELISA for serum samples and culture for sputum samples. Results: Tuberculous lesions were detected in 184 of 10,903 camels (1.7%). The ELISA results revealed that of the 184 examined camel serum samples, 124 (67.39%) were positive and all 20 camel serum samples that had no associated tuberculous lesions were negative. Moreover, only one of 48 (2.08%) human serum samples was positive by ELISA. Mycobacterial culture revealed 112 isolates from the 184 examined camel samples (60.87%), while human sputum sample cultures were all negative. PCR analysis identified the mpb70 gene in three of seven randomly tested samples. Conclusion: Gene sequencing was performed on two samples and the sequences were submitted to the National Center for Biotechnology Information GenBank (accession numbers MF990289 and MG59479). A phylogenetic tree was constructed based on the partial DNA sequences of the mpb70 gene; the similarity between the isolates was 98.1%. The similarities between the two isolates and the standard strains of Mycobacterium bovis in GenBank were 98.1% and 100%, respectively. Further investigation on the antemortem detection of M. bovis infection in camels is needed to decrease public risk.

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“…No overlap was found between the 22 animals with a positive ELISA IDEXX™ and the two animals with positive CCT test results, which compared to similar findings of other studies [33][34][35]. However, in this study six animals (1.50%) reactors on the SCT test were positive on ELISA IDEXX™, which differ from a previous study where no agreement was found between SCT testing and ELISA IDEXX™ [34]. The lack of, or low agreement between, the positive results of the two tests may reflect different elements of the immune response (humoral and cell-mediated immunity) [34,35].…”

Section: Discussionmentioning

confidence: 91%

Study on supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

et al. 2021

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Background Bovine tuberculosis (bTB), is a worldwide disease caused by Mycobacterium bovis (M. bovis). The success of bTB eradication and control programs is based on early detection and the removal of reactors from a herd thus routine testing and cull strategy have been applied globally. Since the late nineteenth century, the Tuberculin Skin Test (TST) has been the primary antemortem test available to support bTB eradication campaigns. Due to the TST limitations in terms of Se and Sp, the credibility of the diagnosis is frequently questioned given the occurrence of false-positive and false-negative reactions, therefore, it is necessary to confirm reactive animals using other methods, ensuring the reliability of the diagnosis. The aim of this study was to evaluate the sensitivity and specificity of a multiplex enzyme-linked immunosorbent assay (ELISA) relative to the tuberculin test used for the diagnosis of tuberculosis in cattle in Brazil. Results Lack of agreement between comparative cervical tuberculin test and ELISA IDEXX TM was observed. The 2 animals positive on the comparative cervical tuberculin test did not react at the ELISA IDEXX TM and 22 negative reactors by comparative cervical tuberculin test were positive by the ELISA IDEXX TM. The ELISA IDEXX TM showed sensitivity that is significantly lower than the official screening test the single cervical tuberculin. ELISA IDEXX TM also detected infected animals and herds undetected by the comparative cervical tuberculin test. The parallel use of comparative cervical tuberculin test and ELISA IDEXX TM increased sensitivity and the feasibility bTB screening. Conclusions The results obtained here suggest that the ELISA IDEXX TM may be a supplemental test for the detection of Mycobacterium bovis infection in regions without routine testing and slaughter, where the disease generally progresses to more advanced stages and antibody responses are likely to be more prevalent. Evidence to support the validation of the ELISA IDEXX™ as a supplemental test for bTB eradication programs was provided.

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Assessment of an ELISA method to support surveillance of bovine tuberculosis in Albania

Cited by 12 publications

References 13 publications

Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Seroprevalence and molecular characterization of Mycobacterium bovis infection in camels (Camelus dromedarius) in the Delta region, Egypt

Study on supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

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