Assessment of acridine orange and SYTO 16 for <i>in vivo</i> imaging of the peritoneal tissues in mice

Udovich, Joshua A.; Besselsen, David G.; Gmitro, Arthur F.

doi:10.1111/j.1365-2818.2009.03153.x

Cited by 16 publications

(16 citation statements)

References 29 publications

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“…Mutagenicity, on the other hand, is a concern. We have recently completed a pilot study in our laboratory evaluating the safety of AO and SYTO 16 in a mouse model 41. The results showed no increase in death rate or cancer incidence in animals treated with a high doses of dye injected into the peritoneal cavity.…”

Section: Commentmentioning

confidence: 99%

In vivo imaging of ovarian tissue using a novel confocal microlaparoscope

Tanbakuchi

Udovich

Rouse

et al. 2010

American Journal of Obstetrics and Gynecology

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Objectives-Develop a clinical confocal microlaparoscope for imaging ovary epithelium in vivo with the long term objective of diagnosing cancer in vivo.Study Design-The first confocal microlaparoscope was developed and used to image the ovaries of twenty-one patients in vivo using fluorescein sodium and acridine orange as the fluorescent contrast agents.Results-The device was tested in vivo and demonstrated to be safe and function as designed. Realtime cellular visualization of ovary epithelium was demonstrated. Conclusions-The confocal microlaparoscope represents a new type of in vivo imaging device.With its ability to image cellular details in real time, it has the potential to aid in the early diagnosis of cancer. Initially, the device may be used to locate unusual regions for guided biopsies. In the long term, the device may be able to supplant traditional biopsies and allow the surgeon to identify early stage ovarian cancer.

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Section: Commentmentioning

confidence: 99%

In vivo imaging of ovarian tissue using a novel confocal microlaparoscope

Tanbakuchi

Udovich

Rouse

et al. 2010

American Journal of Obstetrics and Gynecology

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“…Recent work also demonstrated the feasibility of ex vivo mosaicing confocal microscopy to enable rapid detection of BCCs in skin excision specimens from MMS, using exogenous fluorescent contrast agents to stain nuclear morphology 7–9 . However, use of fluorescent contrast agents for in vivo imaging is still being tested for efficacy and toxicity 10,11 and this method is not yet ready for clinical use. Thus, reflectance‐based RCM imaging continues to advance more rapidly towards clinical applications 12–16 .…”

mentioning

confidence: 99%

In vivo reflectance confocal microscopy of shave biopsy wounds: feasibility of intraoperative mapping of cancer margins

Scope

Mahmood

Gareau

et al. 2010

British Journal of Dermatology

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Background Reflectance confocal microscopy (RCM) images skin at cellular resolution and has shown utility for the diagnosis of nonmelanoma skin cancer in-vivo. Topical application of Aluminum Chloride (AlCl3) enhances contrast in RCM images by brightening nuclei. Objective To investigate feasibility of RCM imaging of shave biopsy wounds using AlCl3 as a contrast agent. Methods AlCl3 staining was optimized, in terms of concentration versus immersion time, on excised tissue ex-vivo. RCM imaging protocol was tested in patients undergoing shave biopsies. The RCM images were retrospectively analyzed and compared to the corresponding histopathology. Results For 35% AlCl3, routinely used for hemostasis in clinic, minimum immersion time was determined to be 1 minute. We identified 3 consistent patterns of margins on RCM mosaic images by varying depths: epidermal margins, peripheral dermal margins, and deep dermal margins. Tumour islands of basal cell carcinoma were identified at peripheral or deep dermal margins, correlating on histopathology with aggregates of neoplastic basaloid cells. Atypical cobblestone or honeycomb pattern were identified at the epidermal margins, correlating with a proliferation of atypical keratinocytes extending to biopsy margins. Conclusions RCM imaging of shave biopsy wounds is feasible and demonstrates the future possibility of intra-operative mapping in surgical wounds.

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“…In 2009, a group from USA also applied AO to some patients with ovarian disease in a clinical study of confocal laser laparoscopic biopsy under FDA approval [53]. The FDA in the USA has provided approval for the application of AO to particular clinical studies after investigation of the acute and chronic toxicity and carcinogenicity of AO using mice [54]. Our study using mice revealed that the LD 50 of AO following intravenous administration was 28-30 mg/kg [18].…”

Section: Toxicity and Carcinogeniety Of Aomentioning

confidence: 85%

Intraoperative Photodynamic Surgery (iPDS) with Acridine Orange for Musculoskeletal Sarcomas

Kusuzaki¹,

Matsubara²,

Satonaka³

et al. 2014

Cureus

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We recently established the new limb salvage modality of acridine orange (AO) therapy (AOT), in an attempt to develop minimally invasive limb salvage surgery with minimal damage of normal tissues and a low risk of local recurrence. The treatment modality consists of intraoperative photodynamic surgery (iPDS) and photodynamic therapy (iPDT), followed by postoperative radiodynamic therapy (RDT) using AO for patients with high-grade malignant musculoskeletal sarcomas. Clinical results have shown that the treatment is associated with a low risk of local recurrence, the risk being almost the same as that following conventional wide resection, and yields superior limb function as compared to that obtained after wide resection.In this review, we present the detailed mechanism of selective accumulation of AO in sarcomas, which is related to the acidic environment and lysosomal acidity of the tumor cells induced by cancer-specific glycolysis not involving the define tricarboxylic acid (TCA) cycle (Warburg's effect). We also describe the clinical uses of AOT and the procedure for intraoperative photodynamic surgery (iPDS) using local administration of AO.

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Assessment of acridine orange and SYTO 16 for in vivo imaging of the peritoneal tissues in mice

Cited by 16 publications

References 29 publications

In vivo imaging of ovarian tissue using a novel confocal microlaparoscope

In vivo imaging of ovarian tissue using a novel confocal microlaparoscope

In vivo reflectance confocal microscopy of shave biopsy wounds: feasibility of intraoperative mapping of cancer margins

Intraoperative Photodynamic Surgery (iPDS) with Acridine Orange for Musculoskeletal Sarcomas

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