In vivo imaging of ovarian tissue using a novel confocal microlaparoscope

Tanbakuchi, Anthony; Udovich, Joshua A.; Rouse, Andrew R.; Hatch, Kenneth D.; Gmitro, Arthur F.

doi:10.1016/j.ajog.2009.07.027

Cited by 61 publications

(43 citation statements)

References 35 publications

(19 reference statements)

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“…New evidence suggests that ovarian and fallopian tube cancers derive instead from cells of Müellerian origin and there is strong evidence that many cancers in the ovary in fact arise from cells originating in the fallopian tube. [8][9][10][11] We have previously demonstrated a rigid confocal microlaparoscope system for imaging the epithelial surface of the ovary [12][13][14][15][16] and our results as well as the findings of several other groups [17][18][19][20] demonstrate that the high resolution en face images obtained with confocal microendoscopes can be used for diagnosing disease.…”

Section: Introductionsupporting

confidence: 67%

Confocal microlaparoscope for imaging the fallopian tube

Rouse

Chambers

et al. 2014

J. Biomed. Opt.

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Abstract. Recent evidence suggests that ovarian cancer can originate in the fallopian tube. Unlike many other cancers, poor access to the ovary and fallopian tubes has limited the ability to study the progression of this deadly disease and to diagnosis it during the early stage when it is most amenable to therapy. A rigid confocal microlaparoscope system designed to image the epithelial surface of the ovary in vivo was previously reported. A new confocal microlaparoscope with an articulating distal tip has been developed to enable in vivo access to human fallopian tubes. The new microlaparoscope is compatible with 5-mm trocars and includes a 2.2-mmdiameter articulating distal tip consisting of a bare fiber bundle and an automated dye delivery system for fluorescence confocal imaging. This small articulating device should enable the confocal microlaparoscope to image early stage ovarian cancer arising inside the fallopian tube. Ex vivo images of animal tissue and human fallopian tube using the new articulating device are presented along with in vivo imaging results using the rigid confocal microlaparoscope system. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Introductionsupporting

confidence: 67%

Confocal microlaparoscope for imaging the fallopian tube

Rouse

Chambers

et al. 2014

J. Biomed. Opt.

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“…Single fiber delivery systems require a compact scanning mechanism at the distal tip [3][4][5][6][7]. Alternatively, this miniaturization can be avoided by scanning the beam at the proximal end of a coherent fiberoptic bundle [8][9][10]. Both single fiber and bundle based endomicroscopy systems can also incorporate miniature focusing optics at the distal tip, either in a fixed position (which defines the axial location of the image plane), or with an axial translation mechanism to obtain optical sections at specific depths within the imaging range [2].…”

Section: Introductionmentioning

confidence: 99%

Axial response of high-resolution microendoscopy in scattering media

Koucky

Pierce

2013

Biomed. Opt. Express

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High-resolution microendoscopy (HRME) uses epi-fluorescence imaging with a coherent fiber-optic bundle to enable in vivo examination of cellular morphology. While the HRME platform has recently gained popularity as a simple alternative to confocal endomicroscopy, the axial response of HRME in thick, scattering tissue has yet to be described quantitatively. These details are important because when analyzing images collected by HRME, out-of-focus light may affect the accuracy of quantitative parameters such as nuclear-to-cytoplasm ratio, which has been proposed as a diagnostic indicator of dysplasia or cancer. In this study we investigated the imaging properties of the HRME system by using phantoms simulating scattering tissue with fluorescently labeled nuclei. We directly compared HRME imaging with confocal endomicroscopy in phantoms and in vivo human tissue. HRME images defocused (deep) objects with apparent diameters and intensity levels that are in agreement with a simple geometric model. Out-of-focus nuclei contribute a relatively low, uniform background level to images which neither leads to the erroneous appearance of large nuclei from deep layers, nor prevents accurate imaging of superficial nuclei with high contrast. Kortum, "A pilot study of low-cost, high-resolution microendoscopy as a tool for identifying women with cervical precancer," Cancer Prev.

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“…Imaging modalities e.g. photoacoustic tomography (PAT), photoacoustic microscopy (PAM), confocal microendoscopy have previously been applied for diagnosis of ovarian cancer [4][5][6], however H&E based histology still remains the gold standard for clinical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Classification and analysis of human ovarian tissue using full field optical coherence tomography

Nandy

Sanders

Zhu

2016

Biomed. Opt. Express

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Abstract:In this study, a full field optical coherence tomography (FFOCT) system was used to analyze and classify normal and malignant human ovarian tissue. 14 ovarian tissue samples (7 normal, 7 malignant) were imaged with the FFOCT system and five features were extracted by analyzing the normalized image histogram from 56 FFOCT images, based on the differences in the morphology of the normal and malignant tissue samples. A generalized linear model (GLM) classifier was trained using 36 images, and sensitivity of 95.3% and specificity of 91.1% was obtained. 20 images were used to test the model, and a sensitivity of 91.6% and specificity of 87.7% was obtained. Walch, "High-mass-resolution MALDI mass spectrometry imaging of metabolites from formalin-fixed paraffinembedded tissue," Nat.

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In vivo imaging of ovarian tissue using a novel confocal microlaparoscope

Cited by 61 publications

References 35 publications

Confocal microlaparoscope for imaging the fallopian tube

Confocal microlaparoscope for imaging the fallopian tube

Axial response of high-resolution microendoscopy in scattering media

Classification and analysis of human ovarian tissue using full field optical coherence tomography

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