Assessment of a phosphorus fractionation method for soils: problems for further investigation

Potter, Robert; Jordan, Carl F.; Guedes, Rosa; Batmanian, G.J.; Han, Xingguo

doi:10.1016/0167-8809(91)90128-k

Cited by 31 publications

(15 citation statements)

References 17 publications

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“…All soil phosphorus fractions were analyzed using the ammonium molybdate-ascorbic acid method described by Murphy and Pviley (1962) as modified by Potter et al (1993). All samples were analyzed for orthophosphate using a Technicon automated analyzer (Bran and Luebbe, Buffalo Grove, IL).…”

Section: Phosphate Determinationmentioning

confidence: 99%

“…The other replicate was extracted immediately with 0.5 mol/L NaHCO 3 , end over end, for 18 h. Subsamples of the nonchloroformtreated bicarbonate extracts were acidified with 1.8 mol/L H 2 SO 4 in order to precipitate the organic compounds out of solution (Tiessen et al 1984;Potter et al 1993). These acidified subsamples were, then analyzed for PO 4 -P, which is equivalent to exchangeable (labile) inorganic P (P ; ) in the soil (Tate 1984(Tate , 1985Tiessen et al 1984;Wagar et al 1986;Schoenau et al 1989).…”

Section: Phosphorus Fractionationmentioning

confidence: 99%

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The Effects of Disturbance Events on Labile Phosphorus Fractions and Total Organic Phosphorus in the Southern Appalachians

Wright

Coleman

1999

Soil Science

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Changes in labile soil phosphorus pools, together with changes in organic matter and total organic soil phosphorus levels, were measured following rhododendron harvest and hurricane windthrow events at the Coweeta Long Term Ecological Research (LTER) site. Seasonal soil samples were 1 taken every 3 months from June 1996 to September 1997. Soil cores were taken along four transects within each study area at distances approximately 1, 5, and 15 m upslope from the stream channel. Levels of resin P were low for all sites and sampling dates (<5 [jug PO 4 -P g soil" 1 ). Levels of bicarbonate inorganic P were <8 (jig PO 4 -P g soil" 1 for all sites and sampling dates, whereas bicarbonate organic P levels were somewhat higher and ranged from 6.5 to 26.6 jjig PO 4 -P g soil" 1. Microbial P levels were the most variable and ranged from 4.5 to 25.8 |xg PO 4 -P g soil" 1. In contrast, total organic P levels were high, 149 to 348 (jug PO 4 -P g soil" 1. For the 3 years after the disturbance events, there were no significant changes in labile phosphorus pools or in total organic phosphorus levels in the soil. This lack of significant differences is likely attributable to the low phosphorus, high P-sorbing status of the soils at Coweeta and the lack of erosion that followed these disturbance events. (Soil Science 1999;164:391-402)

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Section: Phosphate Determinationmentioning

confidence: 99%

Section: Phosphorus Fractionationmentioning

confidence: 99%

The Effects of Disturbance Events on Labile Phosphorus Fractions and Total Organic Phosphorus in the Southern Appalachians

Wright

Coleman

1999

Soil Science

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“…Soils with high P sorption capacities may have lower Kp values (Hedley and Stewart 1982). When bicarbonate is used as a soil extractant, erratic Pm values obtained from acidic and highly weathered soils are attributed to high P sorption capacities (Potter et al 1991). Because of the variation of P sorption in different soils, some authors have omitted attempts to correct for this factor (Selles et al 1995).…”

Section: Resultsmentioning

confidence: 99%

“…McLaughlin et al (1986) found that hexanol is just as effective as chloroform and is safer because chloroform is a suspected carcinogen. A solution of 0.5M NaHCO 3 is most often used for extracting Pm from soil biomass (Potter et al 1991, Joergensen et al 1995. When comparing various extractants in soil biomass studies, Brookes et al (1982) reported that 0.5M NaHCO 3 adjusted to pH 8.5 was the most satisfactory.…”

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confidence: 99%

Evaluation of effect of different sterilization methods on soil biomass phosphorus extracted with NaHCO3

Sinegani¹,

Hosseinpur²

2010

PLANT SOIL ENVIRON

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Soil microbial biomass is a dynamic force driving soil phosphorus cycling in soils. The temperature, time and method for killing soil organisms in soil biomass P determination are so important factors that affect the results. The aim of this study was to compare some methods of soil sterilization and soil microbial P (Pm) release in extraction with NaHCO 3 . Five samples of calcareous soils in three replicates were incubated in field capacity and 28°C. The texture of soils differed from loamy sand to sandy loam. They had 10-15% equivalent calcium carbonate, 1-3% organic carbon and 40-90 mg/kg available P content. After 1, 10, 30, and 50 days of incubation a portion of each soil was sterilized by five methods (chloroform fumigation, autoclaving at 121°C for 0.5 h, oven drying at 70°C for 48 h, oven drying at 180°C for 2 h, and ultrasonification). Phosphorus of the sterilized and control soils were extracted with 0.5M NaHCO 3 (pH 8.5) and determined spectrophotometrically as blue molybdate-phosphate complexes under partial reduction with ascorbic acid. Results showed that the method of soil sterilization and soil type had significant effects on biomass P estimated in soils (P < 0.01). There were no significant differences between extracted biomass P at chloroform fumigation, autoclaving at 121°C for 0.5 h, and oven drying at 70°C for 48 h. However biomass P was significantly more extracted from soil by oven drying at 180°C for 2 h and by ultrasonification. Fixation of Pm in soil during the extraction period can reduce the precision of biomass P estimates. This effect was more obvious in biomass P determination by fumigation and oven drying at 70°C for 48 h. Biomass P determined by these methods and autoclaving had positive and significant correlations with soil sand contents, respectively due to long sterilization period and temperature. Ultrasonification released more P from these calcareous soils especially at the start of soil incubation. It released higher microbial P and reduced P sorption in soils; biomass P determined by this method compared to other methods had relatively higher correlations with fungi, glumales spore and P solubilizing bacteria numbers in soil.

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“…The modestly new methodology is based on a strong base anion exchanger with the active group of trimethyl benzyl ammonium. In terms of forest soils generally characterised by low pH levels, the essential advantage of bicarbonate resin must be highlighted: unlike Cl-resin, the HCO 3 -resin chosen simulates the effect of the plant roots under acid conditions more accurately (SOON 1990;POTTER et al 1991). Because of the important roles of the pH level of the solutions measured and the contents of arsenates, silicates, and germanium compounds, the absorbances of phosphate-molybdate complexes were recommended not to be measured after reduction with ascorbic acid.…”

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confidence: 99%

Untitled

2022

Soil & Water Res.

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Abstract:The anion-exchange resin (AER) method for the estimation of bioavailable phosphorus was slightly optimised for soils with high contents of readily soluble organic compounds as well as for those having very different clay and sand contents. The method based on a strong base anion exchanger, the active group of trimethyl benzyl ammonium, the field moist soil, and the enclosure of resin beds in a bag was very different soils under relatively uniform outer conditions where the determination of the absorbance of (blue) phosphate-molybdate complex at 870 nm is used. The motivation of the proposed method comes from distinctive features of forest soils where marked differences are commonly encountered between clay/silt/sand contents, pH-values, and the contents of organic substances. A modestly new methodology was applied for the evaluation of seasonal phosphorus dynamics in a forest environment. The recommended rapid assay for available phosphorus was statistically compared with other procedures. Considerable seasonal effects on the bioavailable P contents were demonstrated.Keywords: soil phosphorus; forest soils; available P determination 118Soil & Water Res., 1, 2006 (4): 117-126 ELRASHIDI et al. 1975SIBBESEN 1978) that AER phosphorus correlates highly with the uptake of phosphates by plants. Methodological development of the bioavailable phosphorus content measurements using AER in bags is useful for forest soils from the viewpoint of their distinctive features such as a greater quantity of humic substances with markedly different qualities, easily soluble organic compounds, conspicuous organic horizons, distinctive biota, frequently a higher degree of rockiness and stoniness, lower soil reaction, and more extreme mobility of elements. In addition, each of the methods for bioavailable P determinations may result in the mobilisation of not only available phosphates (BISSANI et al. 2002) but also of part of the non-mobile phosphorus pool in the soil sample. Alternatively, there exist available methods that eliminate this disadvantage, out of which the use of isotopical solutions of 32 P (OLSEN & SOMMERS 1982), water-extraction methods based on deionised water ( VAN DER PAAUW 1971), and AER methods are the most relevant for the routine use. The use of isotopical solutions of P is negatively influenced by both short-term and long-term phosphate resorptions, the amount of phosphates extracted by deionised water being extremely low and the anion-exchange resin methods before usage of polyester-netting bags being time consuming. With respect to the facts and to the distinctive features of forest soils, resin-extractable phosphates were studied. The particular analytical steps were derived from the facts that the amount of anion resin-extractable phosphorus used depends upon the time required for adsorption, the concentration of the resin, and the concentration of phosphates.The following analytical limitations of particular extractions were tested: (i) the extremely low amount of phosphates extracted by deionise...

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Assessment of a phosphorus fractionation method for soils: problems for further investigation

Cited by 31 publications

References 17 publications

The Effects of Disturbance Events on Labile Phosphorus Fractions and Total Organic Phosphorus in the Southern Appalachians

The Effects of Disturbance Events on Labile Phosphorus Fractions and Total Organic Phosphorus in the Southern Appalachians

Evaluation of effect of different sterilization methods on soil biomass phosphorus extracted with NaHCO3

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