Abstract. Human umbilical vein endothelial (HUVE) cells have been previously reported to express the genes for the A and B chains of PDGF and to secrete PDGF-related factors into culture media. Antihuman PDGF IgG affinity chromatography was used to purify PDGF-related activity from HUVE cell-conditioned media . Immunoblot analysis of the affinity-purified proteins with anti-PDGF IgG and antibodies specific for the A or B chain peptides of PDGF combined with chemotactic and mitogenic assays revealed that the major PDGF immunorelated molecule secreted by HUVE cells is a monomer of ti 36-38 kD and that

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N-acetylglucosamine (O-GlcNAc) modification on serine or threonine residues of cytoplasmic and nuclear proteins has become a more recognized intracellular covalent modification. Removal of this modification is carried out by N-acetyl-beta-glucosaminidase (O-GlcNAcase). Since little information exists on monoglycosylation and O-GlcNAcase activity in mitogenic systems, we investigated O-GlcNAcase activity in primary breast tumors compared to matched normal adjacent breast tissue and examined enzymatic activity in relationship to the level of protein monoglycosylation. Using a variation of the acidic hexosaminidase activity assay, we demonstrated an increase in both O-GlcNAcase and lysosomal hexosaminidase activity in breast tumor tissue compared to matched adjacent tissue. Although no clear correlation with tumor grade or type was apparent among the samples examined (12 matched pairs), the increase in O-GlcNAcase and lysosomal hexosaminidase activity in tumor tissue was consistently elevated and statistically significant (P<0.05). Protein monoglycosylation was evaluated using immunoblotting, affinity blotting, and radioactive labeling. While the variety of modified proteins was greater in tumor tissue compared to adjacent tissue, the total amount of O-GlcNAc monoglycosylation was significantly decreased in the tumor tissue especially on proteins in the molecular mass range of 45-65 kDa. O-GlcNAcase may be involved in the selective removal of O-GlcNAc on certain proteins in breast tumor tissue.

Two hot dry rock geothermal energy reservoirs were created by hydraulic fracturing of Precambrian granitic rock on the west flank of the Valles Caldera, a dormant volcanic complex, in the Jemez Mountains of northern New Mexico. Heat was extracted in a closed‐loop mode of operation, injecting water into one well and extracting the heated water from a separate production well. The first reservoir was produced by fracturing the injection well at a depth of 2.75 km, where the indigenous rock temperature was 185°C. The relatively rapid decline in temperature of the water produced from the first reservoir, 100°C in 74 days, indicated an effective fracture radius of about 60 m with an average thermal power extracted of 4 MW. A second, larger reservoir was created by refracturing the injection well 180 m deeper. Downhole measurements of water temperature at the reservoir outlet as well as temperatures inferred from chemical geothermometry showed that the thermal decline of this reservoir was negligible; the effective heat transfer area of the new reservoir must be at least 45,000 m2, nearly 6 times larger than the first reservoir. In addition, reservoir residence time studies employing visible dye tracers indicated that the mean volume of the second reservoir is 9 times larger. Other measurements showed that flow impedances were low and that downhole water losses from these reservoirs should be manageable. The geochemistry of the produced water was essentially benign, with no scaling problems apparent. Moreover, the level of induced seismic activity was insignificantly small.

Scarification in sulfuric acid consistently increased germination of Opuntia edwardsii sp. nov, O. discata Griffiths, and O. lindheimeri Engelm. ♯ OPULI seeds over that of untreated seeds. Optimum constant temperatures for germination were generally 25 to 35 C and germination was not enhanced by alternating temperatures. There was a trend for increased germination following leaching in water for 12 h which suggested the presence of chemical germination inhibitors. Seeds passed through the digestive tracts of cattle exhibited average germination percentages that were 1.5 times greater than seeds removed from ripe fruits.

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