Assessment of a Multiplex PCR for the Simultaneous Diagnosis of Intestinal Cryptosporidiosis and Microsporidiosis

Epidemiological and clinical study of microsporidiosis in French kidney transplant recipients from 2005 to 2019: TRANS‐SPORE registry

“…15 In a recent monocentric study carried out in France, Moniot et al reported that 2.4% of overall diarrhea (i.e., infectious and non-infectious) in kidney transplant patients was attributable to E. bieneusi. 16 These results are in accordance to another recent French study in which microsporidiosis accounted for 11.1% of infectious diarrhea in kidney transplant patients. 17 Second, until recently, microsporidiosis was mainly diagnosed using light microscopy with staining-based techniques, but it might lack sensitivity and specificity and require knowledge.…”

“…These results are in accordance to another recent French study in which microsporidiosis accounted for 11.1% of infectious diarrhea in kidney transplant patients 17 . Second, until recently, microsporidiosis was mainly diagnosed using light microscopy with staining‐based techniques, but it might lack sensitivity and specificity and require knowledge 16 . Homemade PCR analysis represents an obvious choice, and although it is more expensive than microscopy, it allows for automation.…”

“…In a review article, the prevalence of E. bieneusi infection in HIV patients from both developed or developing countries ranged from 2% to 78% and from 2.5% to 51%, respectively 15 . In a recent monocentric study carried out in France, Moniot et al reported that 2.4% of overall diarrhea (i.e., infectious and non‐infectious) in kidney transplant patients was attributable to E. bieneusi 16 . These results are in accordance to another recent French study in which microsporidiosis accounted for 11.1% of infectious diarrhea in kidney transplant patients 17 .…”

Microsporidiosis after liver transplantation: A French nationwide retrospective study

“…In the prospective study by Moniot and coll., among 82 French kidney transplants presenting with diarrhea, microsporidiosis and cryptosporidiosis accounted for 2.4% and 7.3%, respectively. 19 However, prevalences were probably underestimated, as this work did not distinguish between infectious and non-infectious diarrhea.…”

“…Gastrointestinal microsporidiosis can be diagnosed using stool microscopy of at least three stool samples with chromotrope and chemofluorescent stains or immunofluorescence. 2,17 More recently, in house and commercialized PCR assays for detection and identification to the species level exhibit excellent diagnostic performance, and some of them allow simultaneous detection of Cryptosporidium sp 2,[18][19][20] Infection with E bieneusi is usually restricted to the intestinal tract, and is a major cause of acute or persistent diarrhea. Clinical manifestations also include weight loss, nausea, and abdominal pain.…”