Assessment of a bioactive compound for its potential antiinflammatory property by tight junction permeability

Bai, Young Hoon; Pak, Sok Cheon; Lee, Seung Hoo; Bae, Chun Sik; Prosser, Colin G.; Stelwagen, K.; Lee, Jin Hee; Park, Soon Duck

doi:10.1002/ptr.1772

Cited by 8 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another conceivable mode of action for B. lactis is that they modulate directly the function of epithelial cells. It has been reported that different probiotic strains, including the VSL3# preparation, probiotic bacterial lysates or conditioned medium increase epithelial barrier function as measured with TER [25–27]. In addition, at least some probiotics stabilize tight junctions and induce mucin secretion in epithelial cells [25].…”

Section: Discussionmentioning

confidence: 99%

Live probioticBifidobacterium lactisbacteria inhibit the toxic effects induced by wheat gliadin in epithelial cell culture

Lindfors

Blomqvist

Juuti‐Uusitalo

et al. 2008

Clinical and Experimental Immunology

178

102

View full text Add to dashboard Cite

SummaryWheat gliadin induces severe intestinal symptoms and small-bowel mucosal damage in coeliac disease patients. At present, the only effective treatment for the disease is a strict life-long gluten-free diet. In this study we investigated whether probiotics Lactobacillus fermentum or Bifidobacterium lactis can inhibit the toxic effects of gliadin in intestinal cell culture conditions. The ability of live probiotics to inhibit peptic-tryptic digested gliadin-induced damage to human colon cells Caco-2 was evaluated by measuring epithelial permeability by transepithelial resistance, actin cytoskeleton arrangements by the extent of membrane ruffling and expression of tight junctional protein ZO-1. B. lactis inhibited the gliadin-induced increase dose-dependently in epithelial permeability, higher concentrations completely abolishing the gliadin-induced decrease in transepithelial resistance. The same bacterial strain also inhibited the formation of membrane ruffles in Caco-2 cells induced by gliadin administration. Furthermore, it also protected the tight junctions of Caco-2 cells against the effects of gliadin, as evinced by the pattern of ZO-1 expression. We conclude thus that live B. lactis bacteria can counteract directly the harmful effects exerted by coeliac-toxic gliadin and would clearly warrant further studies of its potential as a novel dietary supplement in the treatment of coeliac disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Live probioticBifidobacterium lactisbacteria inhibit the toxic effects induced by wheat gliadin in epithelial cell culture

Lindfors

Blomqvist

Juuti‐Uusitalo

et al. 2008

Clinical and Experimental Immunology

178

102

View full text Add to dashboard Cite

show abstract

“…It has been hypothesised that probiotics have anti-inflammatory effects in human IBD and preserve intestinal epithelial integrity (58) . A number of in vitro studies using epithelial monolayers have demonstrated that probiotics have improved epithelial barrier function following Escherichia coli infection or incubation with proinflammatory cytokines (59)(60)(61)(62) . Several in vivo studies have demonstrated that probiotic therapy may change the expression of TJ proteins (57,63) and decrease paracellular permeability by increasing the phosphorylation of TJ proteins, such as ZO-1, claudin-1, or occludin in dextran-sodium sulfate-induced colitis (acute model) mice (57,63,64) and IL-10 -/-(chronic model) mice (65,66) .…”

Section: Nutrition Research Reviewsmentioning

confidence: 99%

Intestinal barrier dysfunction: implications for chronic inflammatory conditions of the bowel

Miner-Williams

Moughan

2016

Nutr. Res. Rev.

View full text Add to dashboard Cite

The intestinal epithelium of adult humans acts as a differentially permeable barrier that separates the potentially harmful contents of the lumen from the underlying tissues. Any dysfunction of this boundary layer that disturbs the homeostatic equilibrium between the internal and external environments may initiate and sustain a biochemical cascade that results in inflammation of the intestine. Key to such dysfunction are genetic, microbial and other environmental factors that, singularly or in combination, result in chronic inflammation that is symptomatic of inflammatory bowel disease (IBD). The aim of the present review is to assess the scientific evidence to support the hypothesis that defective transepithelial transport mechanisms and the heightened absorption of intact antigenic proinflammatory oligopeptides are important contributing factors in the pathogenesis of IBD.

show abstract

“…Certain strains of S. cerevisae also seem to have beneficial effect on the gastrointestinal tract. Lifree, made of fruits fermented using a mixture of probiotics, including S. Cerevisae, promotes tight junction formation [74]. S. cerevisae UFMG 905 preserves gut barrier and immune system during the intestinal obstruction injury [75].…”

Section: Gastrointestinal Mucosal Barriermentioning

confidence: 99%

Protection and Restitution of Gut Barrier by Probiotics: Nutritional and Clinical Implications

Rao¹,

Samak²

2013

CNF

203

View full text Add to dashboard Cite

Probiotics are beneficial bacteria present in various dietary components and many of these colonize in the human and animal intestine. In the gut probiotics help the host by assisting in maintenance of normal mucosal homeostasis. Probiotics not only help maintain normal function of the gut mucosa, but also protect mucosa from injurious factors such as toxins, allergens and pathogens. The beneficial effect of probiotics is mediated by multiple mechanisms, including cytoprotection, cell proliferation, cell migration, resistance to apoptosis, synthesis of proteins and gene expression. One of the important cytoprotective effects of probiotics in the intestinal mucosa is to strengthen the epithelial tight junctions and preservation of mucosal barrier function. Probiotics not only enhance barrier function by inducing synthesis and assembly of tight junction proteins, but also preventing disruption of tight junctions by injurious factors. Bioactive factors released by probiotics trigger activation of various cell signaling pathways that lead to strengthening of tight junctions and the barrier function. This article reviews and summarizes the current understanding of various probiotics that are involved in the protection of gut barrier function, highlights the cellular and molecular mechanisms involved in the protective effect and addresses the clinical implications of probiotic supplementation.

show abstract

Assessment of a bioactive compound for its potential antiinflammatory property by tight junction permeability

Cited by 8 publications

References 20 publications

Live probioticBifidobacterium lactisbacteria inhibit the toxic effects induced by wheat gliadin in epithelial cell culture

Live probioticBifidobacterium lactisbacteria inhibit the toxic effects induced by wheat gliadin in epithelial cell culture

Intestinal barrier dysfunction: implications for chronic inflammatory conditions of the bowel

Protection and Restitution of Gut Barrier by Probiotics: Nutritional and Clinical Implications

Contact Info

Product

Resources

About