Assessing the feasibility of low temperature XAFS experiments at Indus-2, India: First results

Ramanan, Nitya; Rajput, Parasmani; Jha, S. N.; Lahiri, Debdutta

doi:10.1016/j.nima.2015.02.001

Cited by 2 publications

(2 citation statements)

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“…This mandates advanced supportive technologies, e.g. high photon flux (Fischetti et al, 2004;Gauthier et al, 1999;Cotelesage et al, 2012a,b;Proux et al, 2005;Adachi et al, 2001) and efficient fluorescence detectors (Cramer et al, 1988) for high signal statistics, fast scanning monochromators for short exposure time (Khalid et al, 2011) and cryo-cooling for arresting radiation damage (Ramanan et al, 2015). These experimental facilities are moderately satisfied at BL-9, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Assessing the prospect of XAFS experiments of metalloproteins under in vivo conditions at Indus-2 synchrotron facility, India

Lahiri¹,

Agrawal²,

Chandravanshi³

et al. 2023

J Synchrotron Radiat

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The feasibility of X-ray absorption fine-structure (XAFS) experiments of ultra-dilute metalloproteins under in vivo conditions (T = 300 K, pH = 7) at the BL-9 bending-magnet beamline (Indus-2) is reported, using as an example analogous synthetic Zn (0.1 mM) M1dr solution. The (Zn K-edge) XAFS of M1dr solution was measured with a four-element silicon drift detector. The first-shell fit was tested and found to be robust against statistical noise, generating reliable nearest-neighbor bond results. The results are found to be invariant between physiological and non-physiological conditions, which confirms the robust coordination chemistry of Zn with important biological implications. The scope of improving spectral quality for accommodation of higher-shell analysis is addressed.

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Section: Introductionmentioning

confidence: 99%

“…These experimental facilities are moderately satisfied at BL-9, e.g. flux ' 10 11 photons s À1 , four-element silicon VORTEX detector (Barkan et al, 2003), QEXAFS (Poswal et al, 2016) and cryocooling (Ramanan et al, 2015), which encouraged XAFS measurements of Cu protein (powder) at this beamline (Dutta Gupta et al, 2021).…”

Section: Introductionmentioning

confidence: 99%