Assessing the determination of salivary electrolytes and anti-Ro and anti-La antibodies for the diagnosis of Sjogren s syndrome (SS)

Busamia, Beatriz; Gonzales-Moles, Miguel-Angel; Mazzeo, M; Linares, J.A.; Demarchi, Marcela; Gobbi, Carla; Albiero, Eduardo; Finkelberg, A.

doi:10.4317/medoral.15.e437

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…A decrease in saliva flow rate is a clinical characteristic of pSS, but the possible changes in the salivary constituents, such as SSA/B, are still unknown. Recently, researchers have detected several auto antibodies expressed in the saliva of patients with pSS, such as anti-alpha-fodrin, anti-M3R, and anti-SSA/B antibodies ( 17 - 19 ). Their roles in the diagnosis of SS have also been assessed.…”

Section: Discussionmentioning

confidence: 99%

Role of salivary anti-SSA/B antibodies for diagnosing primary Sjogren’s syndrome

Pan

Li²,

Lü³

et al. 2015

Med Oral

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The diagnosis of primary Sjögren’s syndrome (pSS) is complex, and the saliva test is a potential method to improve the existing diagnostic criteria. Objective: To estimate the diagnostic accuracy of salivary anti-SSA/B antibodies in primary Sjögren’s syndrome (pSS), and to analyze their correlations with clinical and laboratory profiles. Study Design: This study enrolled 100 pSS patients and 140 non-pSS controls, including 40 rheumatoid arthritis (RA) patients, 40 systemic lupus erythematosus (SLE) patients, and 60 healthy controls. Unstimulated whole saliva and stimulated parotid saliva samples were collected from the subjects. Salivary anti-SSA/B antibodies were measured using an enzyme-linked immunosorbent assay (ELISA). Clinical and laboratory data were retrieved from the medical records. Results: In the pSS group, the sensitivity of anti-SSA and anti-SSB antibodies in whole saliva was 49% and 29%, respectively, and the specificity was 87.5% and 95%. The sensitivity of anti-SSA and anti-SSB antibodies in parotid saliva was 32% and 8%, respectively, and the specificity was 95.52% and 97.86%, respectively. In the pSS group, the diagnostic accuracy of anti-SSA/B antibodies in whole saliva was significantly higher than in parotid saliva (p<0.05), but was significantly lower than in serum (p<0.05). The salivary flow rate in the pSS group positive for whole salivary anti-SSA was significantly lower than in the negative group (p<0.05). The prevalence of rheumatoid factor and antinuclear factor were significantly higher in salivary SSB-positive pSS patients than in SSB-negative patients (p<0.05). Conclusions: Compared to parotid saliva, whole saliva is a more suitable diagnostic fluid. Using salivary anti-SSA/B antibodies as a single test item is insufficient given the relatively low sensitivity. Further studies should investigate the possibility of combining tests for different salivary autoantibodies as a method for diagnosing pSS. Key words:Primary Sjögren’s syndrome, salivary diagnostics, anti-SSA autoantibodies, anti-SSB autoantibodies.

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Section: Discussionmentioning

confidence: 99%

Role of salivary anti-SSA/B antibodies for diagnosing primary Sjogren’s syndrome

Pan

Li²,

Lü³

et al. 2015

Med Oral

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“…Biotinylated antigens and immunohistochemical analysis have been used to detect Ro‐ and La‐specific lymphocytes with a plasma cell–like morphology in glandular infiltrates, surrounding acini, and along the basement membrane of the salivary ducts in patients with SS (). Enriched levels of anti‐Ro and/or anti‐La in tears and saliva (IgG and IgA) correlate with higher titers of these antibody specificities (IgG and IgM) in SS patient serum (). Thus, sites of mucosal immunity in patients with SS could reveal critical features about the development of the humoral immune response during disease progression.…”

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confidence: 99%

Antibody‐Secreting Cell Specificity in Labial Salivary Glands Reflects the Clinical Presentation and Serology in Patients With Sjögren's Syndrome

Maier-Moore

Koelsch

Smith

et al. 2014

Arthritis & Rheumatology

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Objectives The serologic hallmark of primary Sjögren’s syndrome (pSS) is IgG antibodies specific for Ro (SSA) and La (SSB). Molecular characteristics of glandular-derived B cells at the site of pSS inflammation have been described; however, parallels between glandular antibody-secreting cells (ASC) and serologic antibody specificities have not been evaluated. We utilized recombinant monoclonal antibody (hmAb) technology to study salivary gland-(SG) derived ASC specificities, evaluating their molecular characteristics and identified IgG antibody specificity. Methods hmAbs were generated from glandular IgG ASC. Heavy and light chain usage and immunoglobulin subclass were analyzed by sequencing. ELISA, indirect immunofluorescence, enzyme immunoassay and 35S immunoprecipitation analysis were used to determine antibody specificity. Results Evaluation of single ASCs from SG biopsies of patients with primary SS or with SS and overlapping SLE revealed significant concordance between serum autoantibody and glandular ASC specificities. Glandular-derived ASC heavy and light chains were extensively somatically hypermutated, indicative of antigen-driven responses. Specifically, we produced the first fully human monoclonal autoantibodies derived from salivary glands in this study. Conclusions Salivary glands in SS patients are a site for antibody production, which extend beyond the canonical Ro and/or La SS specificities. Furthermore, we demonstrate that glandular antibody production strongly reflects the serological humoral response in the two patients studied herein.

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“…Diagnosing SjS is difficult because of diversity of its initial manifestations and the heterogeneity of the autoimmune markers described during the course of the disease. Consequently, a combined set of biomarkers are assumed to be required to assess more specifically this exocrinopathy such as using proteomics (Fleissig et al , 2009) and salivary anti‐SSA/Ro and anti‐SSB/La (Busamia et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have focused on the assessment of cortisol in the context of salivary gland impairment in primary and secondary SjS and xerostomic patients (Rivera Gómez et al , 2006; Shigeyama et al , 2008; van der Goes et al , 2011). In parallel, other studies examined changes in the salivary sialochemistry of these patients (Mandel, 1980; Pijpe et al , 2007; Busamia et al , 2010). However, to our knowledge, no study has assessed both the cortisol and sialochemistry in this group of patients.…”

Section: Introductionmentioning

confidence: 99%

Sialochemistry and cortisol levels in patients with Sjogren’s syndrome

Miller¹,

Deutsch

Redlich

et al. 2011

Oral Diseases

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Increased whole-salivary output of Cl(-) and K(+) in SjS may reflect release from apoptotic rests of acinar cells after secondary necrosis. Normal levels of salivary Na(+) , Mg(2+) , and (-) argue against concentration effect, deranged tubular function or cortisol (mineralocorticosteroid) effect as the cause for these findings. Increased salivary Ca(2+) levels probably reflect leakage of plasma Ca(2+) through the injured oral mucosa in SjS. In spite of disease-associated stress, salivary cortisol, a stress biomarker, was not increased, suggesting insufficient hypothalamus-pituitary-adrenal (HPA) axis response and/or local consumption of cortisol by lymphocyte infiltrates.

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Assessing the determination of salivary electrolytes and anti-Ro and anti-La antibodies for the diagnosis of Sjogren s syndrome (SS)

Cited by 5 publications

References 17 publications

Role of salivary anti-SSA/B antibodies for diagnosing primary Sjogren’s syndrome

Role of salivary anti-SSA/B antibodies for diagnosing primary Sjogren’s syndrome

Antibody‐Secreting Cell Specificity in Labial Salivary Glands Reflects the Clinical Presentation and Serology in Patients With Sjögren's Syndrome

Sialochemistry and cortisol levels in patients with Sjogren’s syndrome

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