Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate

Stevanović, Milena; Djošić, Marija; Janković, Ana; Nešović, Katarina; Kojić, Vesna; Stojanović, Jovica; Grujić, Svetlana; Bujagić, Ivana Matić; Rhee, Kyong Yop; Mišković‐Stanković, Vesna

doi:10.1021/acsomega.0c01583

Cited by 34 publications

(32 citation statements)

References 59 publications

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“…Additionally, researchers showed enhanced cell adhesion and spreading on the surface of Ti-6Al-4V coated by both HA and Col/HA composite. In another study, HA/chitosan/gentamicin coating deposited by an electrophoretic process on the surface of Ti showed non-toxic character against mouse fibroblasts (L929 cell line) and human lung fibroblasts (MRC-5 cell line), as found by Stevanović et al [65]. In turn, Qiaoxia et al [66] deposited HA/tannic acid composite coating on the Ti modified with TiO 2 nanotubes.…”

Section: Inorganic and Composite Coatingsmentioning

confidence: 78%

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

Kazimierczak

Przekora

2020

Coatings

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The main aim of bone tissue engineering is to fabricate highly biocompatible, osteoconductive and/or osteoinductive biomaterials for tissue regeneration. Bone implants should support bone growth at the implantation site via promotion of osteoblast adhesion, proliferation, and formation of bone extracellular matrix. Moreover, a very desired feature of biomaterials for clinical applications is their osteoinductivity, which means the ability of the material to induce osteogenic differentiation of mesenchymal stem cells toward bone-building cells (osteoblasts). Nevertheless, the development of completely biocompatible biomaterials with appropriate physicochemical and mechanical properties poses a great challenge for the researchers. Thus, the current trend in the engineering of biomaterials focuses on the surface modifications to improve biological properties of bone implants. This review presents the most recent findings concerning surface modifications of biomaterials to improve their osteoconductivity and osteoinductivity. The article describes two types of surface modifications: (1) Additive and (2) subtractive, indicating biological effects of the resultant surfaces in vitro and/or in vivo. The review article summarizes known additive modifications, such as plasma treatment, magnetron sputtering, and preparation of inorganic, organic, and composite coatings on the implants. It also presents some common subtractive processes applied for surface modifications of the biomaterials (i.e., acid etching, sand blasting, grit blasting, sand-blasted large-grit acid etched (SLA), anodizing, and laser methods). In summary, the article is an excellent compendium on the surface modifications and development of advanced osteoconductive and/or osteoinductive coatings on biomaterials for bone regeneration.

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Section: Inorganic and Composite Coatingsmentioning

confidence: 78%

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

Kazimierczak

Przekora

2020

Coatings

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“…Choosing the ideal bone graft depends on several factors such as tissue viability, damage size, graft size, shape, graft volume, biocompatibility, biomechanical capabilities and cost (Guehennec, 2005;Micić, 2020). In order to determine whether a newly developed biomaterial intended for bone implantation conforms to the requirements of biocompatibility, mechanical stability and safety, it must undergo the recommended in vivo testing (Pérez-Sánchez, 2010;Borie, 2017;Stevanović et al, 2018;Stevanović et al, 2020a;Stevanović et al, 2020b;Đošić et al, 2017). Rabbits are one of the most commonly used animal models for exploring bone-implant interactions and for analysing the macrostructure and microstructure in bone composition and remodelling (Kleinschmidt and Hollinger, 1992;Guehennec, 2005).…”

Section: Rabbit a Common Animal Model For Assessing Hard Tissue Biocompatibilitymentioning

confidence: 99%

Animal models in bicompatibility assessments of implants in soft and hard tissues

et al. 2022

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The ethical dilemmas of using animals as in vivo models in preclinical and clinical examinations have been increasingly present in recent decades. Small laboratory animals (rats, rabbits) will continue to be used because they are cost-effective and permit the formation of statistically testable cohort groups; a task that, for financial, maintenance and care reasons, is almost prohibitive for larger animals. Technological advances in the production of new biomaterials for clinical use are enormous, but screening tests and methods used to assess biocompatibility lag behind these advances. The assessment of biological responses is slow and based on millennial recovery mechanisms in eukaryotic organisms. Therefore, the goal of researchers in this field is to re-evaluate old methods of biocompatibility assessment and introduce new methods of evaluation, especially for in vivo testing. In that sense, a revision of the ISO standards was planned and conducted in 2017, which insisted on cytotoxicity testing in cell lines and produced concrete proposals on how biocompatibility should be quantified. In vivo biocompatibility evaluation of biomaterials used for soft tissue recovery commonly utilises rats. Rabbits are recommended for implants used for hard tissues, because of the rabbit?s size, the possibility of implanting the biomaterials on a larger bone surface, and because of the peculiarities of rabbit bone tissue that favours rapid recovery after bone defects and enables easy reading of the results.

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“…An additional advantage of chitosan presence in composite coating can be observed through the slower release of gentamicin during the next 14 days. Prolonged gentamicin release from the hydroxyapatite/chitosan/gentamicin composite coatings makes these coatings great candidates for potential application in the treatment of orthopedic infections [ 68 ]. Composite coatings based on chitosan and hydroxyapatite show excellent biocompatibility, which was confirmed through the formation of new hydroxyapatite layer after only 7 days of immersion in simulated body fluid (SBF) [ 68 , 69 ].…”

Section: Engineering Implant Surfaces To Prevent Microbial Adhesion and Infectionmentioning

confidence: 99%

“…Prolonged gentamicin release from the hydroxyapatite/chitosan/gentamicin composite coatings makes these coatings great candidates for potential application in the treatment of orthopedic infections [ 68 ]. Composite coatings based on chitosan and hydroxyapatite show excellent biocompatibility, which was confirmed through the formation of new hydroxyapatite layer after only 7 days of immersion in simulated body fluid (SBF) [ 68 , 69 ]. Above everything mentioned before, chitosan has very high position in the field of biomaterials production due to its exceptional properties of easy film forming ability [ 57 , 70 ].…”

Section: Engineering Implant Surfaces To Prevent Microbial Adhesion and Infectionmentioning

confidence: 99%

“…Above everything mentioned before, chitosan has very high position in the field of biomaterials production due to its exceptional properties of easy film forming ability [ 57 , 70 ]. Chitosan, as a cationic polysaccharide, can be used in the electrophoretic deposition process (EPD), one of the most attractive techniques for bioactive coatings production [ 68 , 69 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ]. Lignin (Lig) is a polyphenolic polymer, originated from the various natural sources [ 78 , 79 , 80 ].…”

Section: Engineering Implant Surfaces To Prevent Microbial Adhesion and Infectionmentioning

confidence: 99%

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Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium

2021

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Current trends in biomaterials science address the issue of integrating artificial materials as orthopedic or dental implants with biological materials, e.g., patients’ bone tissue. Problems arise due to the simple fact that any surface that promotes biointegration and facilitates osteointegration may also provide a good platform for the rapid growth of bacterial colonies. Infected implant surfaces easily lead to biofilm formation that poses a major healthcare concern since it could have destructive effects and ultimately endanger the patients’ life. As of late, research has centered on designing coatings that would eliminate possible infection but neglected to aid bone mineralization. Other strategies yielded surfaces that could promote osseointegration but failed to prevent microbial susceptibility. Needless to say, in order to assure prolonged implant functionality, both coating functions are indispensable and should be addressed simultaneously. This review summarizes progress in designing multifunctional implant coatings that serve as carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the implant-tissue interface, while still promoting osseointegration.

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Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate

Cited by 34 publications

References 59 publications

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

Animal models in bicompatibility assessments of implants in soft and hard tissues

Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium

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