Assessing product adulteration of<i>Eurycoma longifolia</i>(Tongkat Ali) herbal medicinal product using DNA barcoding and HPLC analysis

Abubakar, Bashir Mohammed; Salleh, Faezah Mohd; Shamsir, Mohd Shahir; Wagiran, Alina

doi:10.1080/13880209.2018.1479869

Cited by 21 publications

(20 citation statements)

References 51 publications

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“…Previous reports showed that a combination of DNA barcoding and HPLC successfully identified five Fritillariae bulbus species from TCM (Traditional Chinese Medicine) and its effective chemical constituents [49]. The authenticity of Eurycoma longifolia HMPs has been tested by DNA barcoding using the ITS2 marker, and its quality was verified using HPLC [50]. The quality of the famous Curcuma longa L. sold on the Chinese market was also assessed with DNA barcoding by discriminating the species using the ITS/LSU D1/D3 marker and HPLC to determine the chemical compositions [51].…”

Section: Hplc Fingerprinting Of L Pumila Hmpsmentioning

confidence: 99%

Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Tarmizi

Wagiran

Salleh

et al. 2021

Plants

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Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L.pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products.

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Section: Hplc Fingerprinting Of L Pumila Hmpsmentioning

confidence: 99%

Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Tarmizi

Wagiran

Salleh

et al. 2021

Plants

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“…Journal Homepage: https://eurekabiomedical.com/index.php/EHI antimalarials, antidiabetic, anticancer and aphrodisiac (Abubakar et al 2018). El can be consumed by combining its extracts in food or in drinks such as tea and coffee for the people of Malaysia (Ahmad et al 2018) and also the Batak ethnic group in Peadundung village is used as "tea" after activities in the fields (Silalahi and Nisyawati 2015).…”

Section: Eureka Herba Indonesiamentioning

confidence: 99%

“…Eurycoma distribution is limited in Southeast Asia(de Padua et al 1999), however some researchers claim it is also found in South Asia. E. longifolia and E Abubakar et al (2018). stated that DNA bar-coding with ITS2 barcodes can be used as the first screening step for HBP testing containing El.…”

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confidence: 99%

“…Although, El is one that has been bought and sold in Malaysia and is declared an aprosidic drug Abubakar et al (2018). reported that herbal medicinal products (HBP) which were stated to contain El extract, turned out that 27% of the 37% HBP studied were fake.products (HMP) containing El are easily found in the market in the form of raw crude root powder (root powder) or capsules, tablets, or a mixture of tea with coffee in Malaysia ( Rehman et al 2016) and markets in North Sumatra (Silalahi e t al.…”

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Relationships For The Utilization Of Pasak Bumi Plants (eurycoma longifolia Jack.) As A Traditional Medicine And Its Bioactivity

Silalahi

2020

Eu Herb Ina

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Pasak bumi (Eurycoma longifolia Jack.) is a species belonging Simarobuaceae hasbeen long used as traditional medicine, which known very well as aphrosidiac. Thestudies have been conducted to reveal the benefits of E. longifolia throughethnobotany, phyto- chemical, and bioessay approaches, but the review articles on theits utilization and bioactivity limited. This article is based on scien- tific articlespublished on line or off line, then synthesized so that to be information the relationshipbetween use and bioactivity. Ethno- botanyof E. longifolia have been used as amedicine for fever, ma- laria, improve stamina, diabetic, cancer, aphrodisiac, erectiledys- function. Bioactivity of E. longifolia is antiosteoporotic, antimicro- bial,aphrodisiac, anticancer, angiogenesis, and hepatoprotective. Eurycomanone issecondary metabolites in E, longifolia has activi- ty as an antimalaria, antipyretic,aphrodisiac, and cytotoxic. The quassinoids, coumarin and glycosides of El haveactivity increase the production, quality, totality, synthesis and release of spermato-zoa. Eurycoma longifolia is very potential to be developed as an antiosteoporotic andaprosidiac drug, but until now most of it is harvested directly from the forest, so topreserve it, we need to study the cultivation method.

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“…Some of pharmacological activities of E. longifolia were antimicrobial, antidiabetic, cytotoxicity against cancerous cells, drug for sexual insuf iciency, immunomodulator, antiin lammation, etc. (Abubakar et al, 2018). In this article review, we aimed to examine the chemical substances and pharmacological activities of E. longifolia.…”

Section: Introductionmentioning

confidence: 99%

A review of the phytochemical compounds and pharmacological activities of Eurycoma longifolia

Insanu¹,

Pratiwi²,

Fidrianny³

2021

ijrps

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Eurycoma longifolia belongs to Simaroubaceae family. It is a tall tree and has different local name from many countries and this review consisted of their traditional usage, phytochemical compounds and pharmacological activity. A systematic review was conducted to study the scientific work of E. longifolia which published in the last 10 years and minimum 20 articles that published in the last 2 years, published in Pubmed, Scopus etc. also has a digital object identifier (DOI). E. longifolia was a popular traditional medicine. The leaves wasused as supplement for giving birth, its bark as anthelmintic and its roots as antimalaria, laxative, antidiabetic etc.Due to high demand of this plant there are various formula of E. longifolia available in health food market. Many studies have been performed to determine the active constituents and pharmacology activities of E. longifolia. Alkaloid, quassinoid, polyphenols, flavonoid, polysaccharide, triterpenoid were found in E. longifolia. Quassinoids is a major phytochemical compound in E. longifolia which has various types like eurycumanone, eurycomanol, eurycomadilactone and eurylactone. Quassinoids has bitter taste and often found in Simaroubaceae family.Pharmacological activities of E. longifolia such as anti-inflammatory, analgesic, antioxidant, antimicrobial, antidiabetic, anti-osteoporotic activities. The literature review results showed that E. longifolia can be considered as medicinal plant for human. In the future, further studies on mechanism of pharmacology activity of E. longifolia are warranted.

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Assessing product adulteration ofEurycoma longifolia(Tongkat Ali) herbal medicinal product using DNA barcoding and HPLC analysis

Cited by 21 publications

References 51 publications

Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Relationships For The Utilization Of Pasak Bumi Plants (eurycoma longifolia Jack.) As A Traditional Medicine And Its Bioactivity

A review of the phytochemical compounds and pharmacological activities of Eurycoma longifolia

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