Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Abstract

Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L.pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products.

Evaluation of plastid and nuclear DNA markers in barcoding of Aloe saudiarabica, KSA

“…The strength of these sequences and their containment of many areas of difference appeared here, as the A. species closest to A. saudiarabica here had some more diversity than its predecessor in the case of matK gene. Also, the results showed that the pairs of primers ITS1 were not sufficiently specific like ITS2, which often in the first attempts do not give good results, while the trend here is the latest and closest to use and distinguish plant species is through the regions of ITS2 (26,27). In conclusion, it can be said that among the genetic markers on which the study was conducted, the efficiency of the matK and ITS2 sequences appeared to distinguish A. saudiarabica plant, and therefore the study recommends using them to document this rare plant.…”

Authentication of Marantodes pumilum (Blume) Kuntze: A Systematic Review

“…This has become a source of concern for botanical drugs suppliers, as wild M. pumilum is known to grow slowly in its natural habitat. As a result, several research institutions have conducted extensive tissue culture breeding for M. pumilum to keep up with the expanding market demand ( Tarmizi et al, 2021 ).…”

“…(Fagaceae) had no DNA sequence. Additionally, a recent study by Tarmizi et al (2021) effectively used DNA barcoding and HPLC techniques for the identification of cultivated M. pumilum var. alata and var.…”

Chemotaxonomic Classification of Peucedanum japonicum and Its Chemical Correlation with Peucedanum praeruptorum, Angelica decursiva, and Saposhnikovia divaricata by Liquid Chromatography Combined with Chemometrics

“…The genetic authentication-based chromatographic profiling method is an emerging technique that is mainly performed by DNA-barcoding hyphenated chromatographic analysis. The combined method provides species-level accuracy of herbal samples, and therefore, a more reliable classification of those samples can be acquired by chemical analysis [ 19 ]. This technique has been applied to the chemical classification of diverse herbal medicines, such as Arnebia [ 20 ], Fritillaria [ 21 ], Phellodendron [ 22 ], and Daphne [ 23 ] species.…”