Assessing genetic diversity and differentiation in Portuguese coarse-wool sheep breeds with microsatellite markers

Santos-Silva, F.; Ivo, Roland; Sousa, M. C. Oliveira; Carolino, Inês; Ginja, Catarina; Gama, L. T.

doi:10.1016/j.smallrumres.2008.04.006

Cited by 35 publications

(32 citation statements)

References 16 publications

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“…However, in these reports, the analysis was done on 6% denaturing PAGE followed by silver staining, hence direct comparisons cannot be made. The overall level of microsatellite loci variation observed in this study is within the range observed for other exotic sheep breeds (Baumung et al 2006;Peter et al 2007;Legaz et al 2008;Quiroz et al 2008;Santos-Silva et al 2008;Alvarez et al 2009). The highest and lowest allele as well as gene diversity was exhibited by Nellore and Garole sheep, respectively.…”

Section: Discussionsupporting

confidence: 83%

“…The observed differentiation (13.2%) is much higher than that observed between European and Middle eastern (Peter et al 2007), Alpine (Dalvit et al 2008), Portugese coarse wool (Santos-Silva et al 2008), West Balkan Prameka (Cinkulov et al 2008), Baltic (Tapio et al 2005) and Austrian (Baumung et al 2006) sheep but is comparable to Tsigai and Zackel type sheep (Kusza et al 2008). Although there are no strict breeding rules geographical isolation between the investigated sheep from different regions has probably led to the moderate level of differentiation among them.…”

Section: Discussionmentioning

confidence: 60%

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Diversity analysis of sheep breeds from Southern peninsular and Eastern regions of India

Arora

Bhatia

Mishra

et al. 2010

Trop Anim Health Prod

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Information is presented on the genetic diversity and relationship among six Indian sheep breeds/populations belonging to the Southern peninsular and Eastern agroecological zones, based on microsatellite markers. Parameters of genetic variation, viz., allele diversity, observed heterozygosity, gene diversity and population inbreeding estimates, were calculated for the six breeds. The allele diversity ranged from 6.40 to 7.92, whereas the gene diversity varied from 0.617 to 0.727. The highest allele and gene diversity was observed for Nellore sheep, while the lowest was exhibited by Garole breed. Within population inbreeding estimate (F(IS)) revealed a significant deficit of heterozygotes in Deccani, Madgyal, Nellore and Garole, whereas Ganjam and Chhotanagpuri sheep showed an excess of heterozygotes. The contribution of each breed to the total diversity of the breeds was quantified by the Weitzman approach. The marginal loss of diversity incurred with removal of Nellore and Garole breeds was higher (>27%), whereas removal of Deccani breed resulted in lowest loss of diversity (3.84%) from the set. Estimation of the genetic differentiation (F(ST)) and genetic distance (D(A)) between the pairs of breeds revealed a close relationship between Deccani and Madgyal sheep (F(ST) = 0.017; D(A) = 0.080) and greatest demarcation between Madgyal and Garole breeds (F(ST) = 0.110; D(A) = 0.622). The information generated would help in shaping genetic management and conservation programs for the sheep breeds under consideration.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 60%

Diversity analysis of sheep breeds from Southern peninsular and Eastern regions of India

Arora

Bhatia

Mishra

et al. 2010

Trop Anim Health Prod

View full text Add to dashboard Cite

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“…Obtained molecular genetic parameters (Na, Ne, PIC and Hª) obtained from this study were higher than previous research (Farid et al 2000;Arranz et al 2001;Santos-Silva et al 2008;Jyotsana et al 2010;Al-Barzinji et al 2011;Hoda & Marsan 2012;Gaouar et al 2014Gaouar et al , 2015bGaouar et al , 2016aGaouar et al , 2016bYilmaz et al 2014;Kdidi et al 2015;Othman et al 2016). These results indicated that microsatellites used in the present study have a high confidence to reveal genetic diversity for these breeds.…”

Section: Discussioncontrasting

confidence: 73%

New genetic identification and characterisation of 12 Algerian sheep breeds by microsatellite markers

Ameur

Ata

Benyoucef

et al. 2017

Italian Journal of Animal Science

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Exposition of variations between breeds is very important for genetic diversity. Determination of this variation is needed to reveal population structure and relationship between populations and planning national breeding and conservation programmes. This study was carried out in 296 animals from 12 different local sheep breeds (Barbarine, Ouled Djellal, Ifilene, Srandi, Darâa, Rembi, Berbere, Taâdmit, Hamra, Sidaou, Tazegzawt and D'men) reared in different regions of Algeria. Fifteen microsatellite markers were used to determine between breed genetic diversity. The population of 12 sheep breeds studied from Algeria exhibited a high number of alleles (24.67) and polymorphic information content (0.90). Observed heterozygosity values were lower than expected for all molecular markers except INRA0123 locus. Obtained G ST value from the present study indicated that 1.9% of total genetic variation resulted from the differences between the breeds. The present study supplied important information to understand between breed genetic differences. Moreover, it has provided the opportunity to discuss with previously reported results. In light of these findings, it can be said that studied microsatellite markers can be successfully used to determine genetic diversity and population structure in Algerian sheep breeds. ARTICLE HISTORY

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“…When the sampled IST subpopulations are analysed separately (Salamon et al, 2012), F is values are lower. The F is values for other sheep are similar to ones reported for Greek breeds (Ligda et al, 2009), and lower than those reported for Portuguese sheep (Santos-Silva et al, 2008). Unlike in IST, estimated F is values in other populations are most likely caused by breeding practices carried out without knowledge regarding the genetic variants available in flocks of these populations.…”

Section: Diversity and Structure Of Pramenka Sheep Breedssupporting

confidence: 73%

Genetic diversity and differentiation of 12 eastern Adriatic and western Dinaric native sheep breeds using microsatellites

Šalamon

Gutiérrez-Gil

Arranz

et al. 2014

Animal

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Nuclear genetic diversity and differentiation of 341 sheep belonging to 12 sheep breeds from Croatia and Bosnia and Herzegovina were examined. The aim of the study was to provide the understanding of the genetic structure and variability of the analysed pramenka sheep populations, and to give indications for conservation strategies based on the population diversity and structure information. The genetic variation of the sheep populations, examined at the nuclear level using 27 microsatellite loci, revealed considerable levels of genetic diversity, similar to the diversity found in other European indigenous low-production sheep breeds. Population-specific alleles were detected at most loci and in breeds analysed. The observed heterozygosity ranged from 0.643 (in Lika pramenka) to 0.743 (in Vlasic pramenka), and the expected heterozygosity ranged from 0.646 (in Lika pramenka) to 0.756 (in Dalmatian pramenka). Significant inbreeding coefficients were found for half of the populations studied and ranged from 0.040 (Pag island sheep) to 0.091 (Kupres pramenka). Moderate genetic differentiation was found between the studied sheep populations. The total genetic variability observed between different populations was 5.29%, whereas 94.71% of the variation was found within populations. Cres island sheep, Lika pramenka and Istrian sheep were identified as the most distinct populations, which was confirmed by the factorial analysis of correspondence and supported through a bootstrapping adjustment to correct for the difference in the sample sizes. The population structure analysis distinguished 12 clusters for the 12 sheep breeds analysed. However, the cluster differentiation was low for Dalmatian, Vlasic, Stolac and Krk pramenka. This systematic study identified Lika pramenka and Rab island sheep as those with the lowest diversity, whereas Istrian sheep and Pag island sheep had the highest. Conservation actions are proposed for Istrian, Rab and Cres island sheep, Lika and Kupres pramenka because of high estimated coefficients of inbreeding.

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Assessing genetic diversity and differentiation in Portuguese coarse-wool sheep breeds with microsatellite markers

Cited by 35 publications

References 16 publications

Diversity analysis of sheep breeds from Southern peninsular and Eastern regions of India

Diversity analysis of sheep breeds from Southern peninsular and Eastern regions of India

New genetic identification and characterisation of 12 Algerian sheep breeds by microsatellite markers

Genetic diversity and differentiation of 12 eastern Adriatic and western Dinaric native sheep breeds using microsatellites

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