Assessing fungicide resistance in populations of Alternaria in Idaho potato fields

Fairchild, K.; Miles, Timothy D.; Wharton, P. S.

doi:10.1016/j.cropro.2013.03.003

Cited by 69 publications

(45 citation statements)

References 23 publications

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“…In our study, approximately 10 % of the total collected isolates were cyprodinil-resistant. Similar results were reported in Idaho where pyrimethanil-resistant A. alternata isolates from potato fields accounted for 11 % of the total collected sample (Fairchild et al 2013). An interesting phenotype, specifically resistant to the multi-site inhibitor mancozeb, was recorded for the first time in the present study.…”

Section: Discussionsupporting

confidence: 85%

“…Reduced conidial production observed in the above phenotype may contribute to the recorded low population frequencies. Fungicides belonging to the anilinopyrimidines have been successfully used, alone (pyrimethanil) or in mixtures (cyprodinil+fludioxonil) against B. cinerea and other pathogens including A. alternata (Fairchild et al 2013). In our study, approximately 10 % of the total collected isolates were cyprodinil-resistant.…”

Section: Discussionmentioning

confidence: 94%

“…A. alternata strains resistant to benzimidazoles, dicarboxamides, phenylpyrroles and the newest Qo inhibitors and SDHI fungicides have been reported (Sutar et al 2013;Avenot and Michailides 2007;Michailides 2004a, 2004b;Dry et al 2004;Solel et al 1996;Biggs 1994;Hutton 1988;Sitton and Pierson 1982;McPhee 1980). Effectiveness of various fungicides controlling A. alternata and the associated resistance risk has been the subject of many studies worldwide though no data is available on the sensitivity of field populations of the fungus on tomato plants in Greece (Fairchild et al 2013;Bochalyams et al 2012;Leiminger and Hausladen 2011;Gutierrez Chapin et al 2006;Ma and Michailides 2004b).…”

Section: Introductionmentioning

confidence: 97%

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Fitness and cross-resistance of Alternaria alternata field isolates with specific or multiple resistance to single site inhibitors and mancozeb

et al. 2015

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42 field isolates of Alternaria alternata collected from infected tomato fruit of processing plants located in the region of Pelloponisos, South Greece, were characterized in terms of sensitivity to mancozeb, tebuconazole, fludioxonil, iprodione and cyprodinil. Fungitoxicity tests in vitro revealed that 63 % of the isolates tested were sensitive to all the above fungicides whereas the remainder of the isolates exhibited reduced sensitivity to one or more of these fungicides. The observed resistance phenotypes were confirmed by in vivo experiments on artificially inoculated tomato fruit. EC 50 in vitro values ranged from 2.34 to >100 μg/ml for mancozeb, 0.16 to >10 μg/ml for iprodione, 0.43 to 20 μg/ml for tebuconazole, 0.007 to >5 μg/ml for cyprodinil and from 0.002 to >10 μg/ml for fludioxonil. Sensitivity distributions of A. alternata isolates to mancozeb, iprodione, cyprodinil and fludioxonil was bimodal indicating a probable initiation of a discrete shift of the population towards reduced sensitivity to these fungicides. Correlation analysis of respective EC 50 values showed positive cross-resistance relationships between fludioxonil and iprodione while positive correlation was observed between mancozeb and cyprodinil, mancozeb and tebuconazole, and cyprodinil and tebuconazole. Interestingly, a number of A. alternata isolates were specifically resistant to the multi-site inhibitor mancozeb, a fact reported for the first time. Isolates resistant to mancozeb were more aggressive while those with reduced sensitivity to both fludioxonil and iprodione produced significantly less conidia than sensitive ones. A considerable portion of the sample exhibited reduced sensitivity and no apparent fitness penalties, a fact that should be considered in future planning and implementation of anti-resistance strategies.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

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Fitness and cross-resistance of Alternaria alternata field isolates with specific or multiple resistance to single site inhibitors and mancozeb

et al. 2015

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“…A especificidade das acilalaninas, estrobilurinas e carboximidas torna esses fungicidas vulneráveis à seleção de linhagens resistentes de P. infestans e A. solani (Davidse et al, 1983;Pasche et al, 2004;Fairchild et al, 2013). Nesse contexto, o desenvolvimento de misturas conjugando esses fungicidas com produtos inespecíficos de ação tem permitido somar diferentes mecanismos de ação, prolongar a vida útil desses fungicidas e reduzir o risco de ocorrência de resistência Luce, 2014).…”

Section: Resultsunclassified

Controle da requeima e pinta preta da batata por fungicidas e seu reflexo sobre a produtividade e a qualidade de tubérculos

et al. 2016

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RESUMO: A requeima (Phytophthora infestans) e a pinta preta (Alternaria solani) estão entre as doenças mais frequentes e destrutivas da cultura da batata. Com o objetivo de avaliar a ação de diferentes grupos fungicidas no controle dessas doenças, foram realizados dois experimentos em cultivo comercial de batata (cultivares Agata e Monalisa), localizados em Pilar do Sul, São Paulo, nas safras 2008 e 2009. O delineamento experimental utilizado foi o de blocos ao acaso, com 4 repetições, sendo cada parcela de 20 m2. As aplicações foram realizadas com um pulverizador costal, munido de barra de aplicação e pressão constante de 3 Bar. O volume de aplicação variou de 300 a 500 L.ha-1 em função do desenvolvimento da cultura. As variáveis avaliadas para as duas doenças foram: severidade em folhas, área abaixo da curva de progresso da doença (AACPD), produtividade total e comercial, e classificação de tubérculos. Os menores níveis de doença e aumentos significativos na produtividade comercial e qualidade de tubérculos foram observados nas parcelas tratadas com fluopicolide + propamocarbe, dimetomorfe + ametoctradina, mandipropamida + clorotalonil, mandipropamida, fenamidona + propamocarbe, bentiavalicarbe + fluazinam, seguidos de dimetomorfe + clorotalonil, mefenoxam + clorotalonil e famoxadona + cimoxanil + mancozebe para requeima, e azoxistrobina + difenoconazol, picoxistrobina, piraclostrobina + metconazol, trifloxistrobina + tebuconazol, azoxistrobina, boscalida + piraclostrobina, iprodiona + pirimetanil e ciprodinil para pinta preta.

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“…However, loss in the efficacy of several commonly used fungicides for controlling Alternaria spp. has been reported (2)(3)(4)(5)(6). Loss of effective fungicides requires discovery and development of new and safe alternative chemicals.…”

mentioning

confidence: 99%

Ex Vivo Application of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp

Ali

El-Sayed

Patel

et al. 2016

Appl Environ Microbiol

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Bacterial biological control agents (BCAs) are largely used as live products to control plant pathogens. However, due to variable environmental and ecological factors, live BCAs usually fail to produce desirable results against foliar pathogens. In this study, we investigated the potential of cell-free culture filtrates of 12 different bacterial BCAs isolated from flower beds for controlling foliar diseases caused by Alternaria spp. In vitro studies showed that culture filtrates from two isolates belonging to Bacillus subtilis and Bacillus amyloliquefaciens displayed strong efficacy and potencies against Alternaria spp. The antimicrobial activity of the culture filtrate of these two biological control agents was effective over a wider range of pH (3.0 to 9.0) and was not affected by autoclaving or proteolysis. Comparative liquid chromatography-mass spectrometry (LC-MS) analyses showed that a complex mixture of cyclic lipopeptides, primarily of the fengycin A and fengycin B families, was significantly higher in these two BCAs than inactive Bacillus spp. Interaction studies with mixtures of culture filtrates of these two species revealed additive activity, suggesting that they produce similar products, which was confirmed by LC-tandem MS analyses. In in planta pre-and postinoculation trials, foliar application of culture filtrates of B. subtilis reduced lesion sizes and lesion frequencies caused by Alternaria alternata by 68 to 81%. Taken together, our studies suggest that instead of live bacteria, culture filtrates of B. subtilis and B. amyloliquefaciens can be applied either individually or in combination for controlling foliar diseases caused by Alternaria species. Many species in the genus Alternaria cause significant yield and quality losses in food and ornamental crops (1). These species primarily attack aerial plant parts, including foliage, fruits, and stems, and cause a variety of symptoms ranging from necrotic leaf spots to enlarged blighted shoots, eventually resulting in defoliation and loss in yield and quality. Alternaria diseases could be managed with a combination of cultural practices, fungicides, and, if available, genetic resistance. Since many crop cultivars lack sufficient resistance to Alternaria spp., fungicide sprays are the primary means of controlling Alternaria diseases (1). However, loss in the efficacy of several commonly used fungicides for controlling Alternaria spp. has been reported (2-6). Loss of effective fungicides requires discovery and development of new and safe alternative chemicals. Although fungicides with newer chemistry are promising, their introduction into crop production systems is slow, and resistant field isolates of the pathogen usually appear very quickly, within a few seasons of the introduction of a new fungicide (5, 7, 8). As with many other plant pathogens, in most cases fungicide resistance in Alternaria spp. has been attributed to single-site mutations in the target genes (see, e.g., references 9, 10, and 11). Therefore, finding multisite-acting fungicides t...

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Assessing fungicide resistance in populations of Alternaria in Idaho potato fields

Cited by 69 publications

References 23 publications

Fitness and cross-resistance of Alternaria alternata field isolates with specific or multiple resistance to single site inhibitors and mancozeb

Fitness and cross-resistance of Alternaria alternata field isolates with specific or multiple resistance to single site inhibitors and mancozeb

Controle da requeima e pinta preta da batata por fungicidas e seu reflexo sobre a produtividade e a qualidade de tubérculos

Ex Vivo Application of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp

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