Assessing DNA damage in children environmentally exposed to pesticides through using the comet assay and the micronucleus test

Kapka‐Skrzypczak, Lucyna; PosobkiewicM, M; Holownia, Piotr; Niedzwiecka, J.; Sawicki, Krzysztof; Cyranka, Małgorzata; Kruszewski, Marcin

doi:10.1093/eurpub/ckt124.057

Cited by 3 publications

(2 citation statements)

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“…The extent of occupational exposure to these chemicals can be contingent upon a multitude of factors, such as the utilization of personal protective equipment (PPE) encompassing gloves, boots, and glasses, the implementation of precautionary measures like wearing protective clothing and frequent hand washing, the nature of the profession, the accessibility of specialized training for safe handling and usage of pesticides, and the presence of regulatory measures in the form of directives and laws directed at safeguarding workers from exposure to occupational toxins, as well as the undertaking of periodic health monitoring (Mehrpour et al, 2014). Dermal exposure of EDCs often occurs from the plant surface to the farmer's skin (Kapka-Skrzypczak et al, 2011). However, exposure can occur even when wearing protective clothes, as this might be a source of extra exposure (Poet and McDougal, 2002).…”

Section: Occupational Exposure To Edcsmentioning

confidence: 99%

Reproductive toxicity of combined effects of endocrine disruptors on human reproduction

Dutta

Sengupta

Bagchi

et al. 2023

Front. Cell Dev. Biol.

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Confluence of environmental, genetic, and lifestyle variables is responsible for deterioration of human fecundity. Endocrine disruptors or endocrine disrupting chemicals (EDCs) may be found in a variety of foods, water, air, beverages, and tobacco smoke. It has been demonstrated in experimental investigations that a wide range of endocrine disrupting chemicals have negative effects on human reproductive function. However, evidence on the reproductive consequences of human exposure to endocrine disrupting chemicals is sparse and/or conflicting in the scientific literature. The combined toxicological assessment is a practical method for assessing the hazards of cocktails of chemicals, co-existing in the environment. The current review provides a comprehensive overview of studies emphasizing the combined toxicity of endocrine disrupting chemicals on human reproduction. Endocrine disrupting chemicals interact with each other to disrupt the different endocrine axes, resulting in severe gonadal dysfunctions. Transgenerational epigenetic effects have also been induced in germ cells, mostly through DNA methylation and epimutations. Similarly, after acute or chronic exposure to endocrine disrupting chemicals combinations, increased oxidative stress (OS), elevated antioxidant enzymatic activity, disrupted reproductive cycle, and reduced steroidogenesis are often reported consequences. The article also discusses the concentration addition (CA) and independent action (IA) prediction models, which reveal the importance of various synergistic actions of endocrine disrupting chemicals mixtures. More crucially, this evidence-based study addresses the research limitations and information gaps, as well as particularly presents the future research views on combined endocrine disrupting chemicals toxicity on human reproduction.

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Section: Occupational Exposure To Edcsmentioning

confidence: 99%

Reproductive toxicity of combined effects of endocrine disruptors on human reproduction

Dutta

Sengupta

Bagchi

et al. 2023

Front. Cell Dev. Biol.

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“…For example, the GSTP1 gene has been associated with glutathione conjugation detoxification catalyzed by glutathione transferases. In cases where this detoxification process is ineffective, the toxic by-products can cause damage to intracellular molecules [12]. Of note, several GST enzymes expressed in the liver are polymorphic [13] and may impact pesticide metabolism and detoxification [14], potentially affecting normal cell function.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal Damage, Chromosome Instability, and Polymorphisms in GSTP1 and XRCC1 as Biomarkers of Effect and Susceptibility in Farmers Exposed to Pesticides

Aldana-Salazar,

Rangel,

Rodríguez

et al. 2024

IJMS

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In the department of Boyacá, Colombia, agriculture stands as one of the primary economic activities. However, the escalating utilization of pesticides within this sector has sparked concern regarding its potential correlation with elevated risks of genotoxicity, chromosomal alterations, and carcinogenesis. Furthermore, pesticides have been associated with a broad spectrum of genetic polymorphisms that impact pivotal genes involved in pesticide metabolism and DNA repair, among other processes. Nonetheless, our understanding of the genotoxic effects of pesticides on the chromosomes (as biomarkers of effect) in exposed farmers and the impact of genetic polymorphisms (as susceptibility biomarkers) on the increased risk of chromosomal damage is still limited. The aim of our study was to evaluate chromosomal alterations, chromosomal instability, and clonal heterogeneity, as well as the presence of polymorphic variants in the GSTP1 and XRCC1 genes, in peripheral blood samples of farmers occupationally exposed to pesticides in Aquitania, Colombia, and in an unexposed control group. Our results showed statistically significant differences in the frequency of numerical chromosomal alterations, chromosomal instability, and clonal heterogeneity levels between the exposed and unexposed groups. In addition, we also found a higher frequency of chromosomal instability and clonal heterogeneity in exposed individuals carrying the heterozygous GSTP1 AG and XRCC1 (exon 10) GA genotypes. The evaluation of chromosomal alterations and chromosomal instability resulting from pesticide exposure, combined with the identification of polymorphic variants in the GSTP1 and XRCC1 genes, and further research involving a larger group of individuals exposed to pesticides could enable the identification of effect and susceptibility biomarkers. Such markers could prove valuable for monitoring individuals occupationally exposed to pesticides.

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Cytogenetic damage in peripheral blood lymphocytes of children exposed to pesticides in agricultural areas of the department of Cordoba, Colombia

Ruíz-Guzmán

Gómez-Corrales

Cruz-Esquivel

et al. 2017

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Assessing DNA damage in children environmentally exposed to pesticides through using the comet assay and the micronucleus test

Cited by 3 publications

References 0 publications

Reproductive toxicity of combined effects of endocrine disruptors on human reproduction

Reproductive toxicity of combined effects of endocrine disruptors on human reproduction

Chromosomal Damage, Chromosome Instability, and Polymorphisms in GSTP1 and XRCC1 as Biomarkers of Effect and Susceptibility in Farmers Exposed to Pesticides

Cytogenetic damage in peripheral blood lymphocytes of children exposed to pesticides in agricultural areas of the department of Cordoba, Colombia

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