Cytogenetic damage in peripheral blood lymphocytes of children exposed to pesticides in agricultural areas of the department of Cordoba, Colombia

Ruíz-Guzmán, Javier Alonso; Gómez-Corrales, Pamela; Cruz-Esquivel, Ángel; Marrugo‐Negrete, José

doi:10.1016/j.mrgentox.2017.10.002

Cited by 25 publications

(16 citation statements)

References 33 publications

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“…Cytokine shutdown may result in immunosuppression, a process already documented as a consequence of chronic pesticide exposure (21) that may favor not only cancer development but also enhance its aggressiveness (22). Several pesticides are proven to be immunotoxicants, and a variety of deleterious mechanisms can be listed, including reduction in the number and function of immune cells (23), genetic damage in lymphocytes (24) and suppression of Th1 responses (25). TNF-α can control the activity of HNF1 (26), which helps to understand why it was referred as the main transcription factor downregulated by chronic pesticide exposure in our study.…”

Section: Discussionmentioning

confidence: 99%

Toxicoproteomics Disclose Pesticides as Downregulators of TNF-α, IL-1β and Estrogen Receptor Pathways in Breast Cancer Women Chronically Exposed

et al. 2020

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Deleterious effects have been widely associated with chronic pesticide exposure, including cancer development. In spite of several known consequences that pesticides can trigger in the human body, few is known regarding its impact on breast cancer women that are chronically exposed to such substances during agricultural work lifelong. In this context, the present study performed a high-throughput toxicoproteomic study in association with a bioinformatics-based design to explore new putative processes and pathways deregulated by chronic pesticide exposure in breast cancer patients. To reach this goal, we analyzed comparatively non-depleted plasma samples from exposed (n = 130) and non-occupationally exposed (n = 112) women diagnosed with breast cancer by using a label-free proteomic tool. The list of proteins differentially expressed was explored by bioinformatics and the main pathways and processes further investigated. The toxicoproteomic study revealed that women exposed to pesticides exhibited mainly downregulated events, linked to immune response, coagulation and estrogen-mediated events in relation to the unexposed ones. Further investigation shown that the identified deregulated processes and pathways correlated with significant distinct levels tumor necrosis factor alpha and interleukin 1 beta in the blood, and specific clinicopathological characteristics pointed out by bioinformatics analysis as adipose-trophic levels, menopause and intratumoral clots formation. Altogether, these findings reinforce pesticides as downregulators of several biological process and highlight that these compounds can be linked to poor prognosis in breast cancer.

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Section: Discussionmentioning

confidence: 99%

Toxicoproteomics Disclose Pesticides as Downregulators of TNF-α, IL-1β and Estrogen Receptor Pathways in Breast Cancer Women Chronically Exposed

et al. 2020

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“…Deficiencies in processes such as tumor evasion prevent one's immune system from recognizing and disposing of malignant tumors. Importantly, recent studies have also linked exposure to atrazine with increased DNA damage in fish [59] and mammals [60], including humans [44,61]. As previously mentioned, this aspect of atrazine toxicity is likely to be connected with the increase in the formation of ROS caused by the reduced expression of cytochrome p450 family genes, hence leading to increased DNA damage.…”

Section: Atrazine: Standard Herbicide Potential Carcinogen and Envimentioning

confidence: 96%

Potential Health Risks Linked to Emerging Contaminants in Major Rivers and Treated Waters

Kessler

Dawley

Crow

et al. 2019

Water

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The presence of endocrine-disrupting chemicals (EDCs) in our local waterways is becoming an increasing threat to the surrounding population. These compounds and their degradation products (found in pesticides, herbicides, and plastic waste) are known to interfere with a range of biological functions from reproduction to differentiation. To better understand these effects, we used an in silico ontological pathway analysis to identify the genes affected by the most commonly detected EDCs in large river water supplies, which we grouped together based on four common functions: Organismal injuries, cell death, cancer, and behavior. In addition to EDCs, we included the opioid buprenorphine in our study, as this similar ecological threat has become increasingly detected in river water supplies. Through the identification of the pleiotropic biological effects associated with both the acute and chronic exposure to EDCs and opioids in local water supplies, our results highlight a serious health threat worthy of additional investigations with a potential emphasis on the effects linked to increased DNA damage.

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“…The populations with the risk of being highly exposed to pesticides are primarily farmers and sprayers, and especially so in low‐ to middle‐income countries where there is little public concern for proper handling and protection (Jeyaratnam, 1990; Jors et al, 2018). It has also been shown that farmers' family members, although not being active as farmers, can be exposed to increased levels of pesticides (Alvarado‐Hernandez et al, 2013; Ruiz‐Guzman et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro cytotoxicity and genotoxicity of single and combined pesticides used by Bolivian farmers

Cuenca

Galvão

Endirlik

et al. 2021

Environ and Mol Mutagen

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We previously showed that farmers in Bolivia are exposed to many pesticides, some at elevated levels, and that this was associated with increased risk of genetic damage. To improve the understanding of possible mixture effects, the cytotoxicity and genotoxicity of pesticides were studied in vitro using human liver HepG2 cells. The studied pesticides were 2,4‐D, chlorpyrifos, cypermethrin, glyphosate, methamidophos, paraquat, profenofos, and tebuconazole. Three mixtures (U1, U2, and U3) were based on profiles of urinary pesticide metabolites and one mixture on the most frequently used pesticides (S1). The results showed that paraquat and methamidophos were the most cytotoxic pesticides (EC50 ≤0.3 mM). Paraquat, chlorpyrifos, tebuconazole, and the U1, U2, and U3 mixtures, which contained a large proportion of either chlorpyrifos or tebuconazole, significantly increased intracellular ROS levels. Most pesticides activated DNA damage signaling through proteins Chk1 and H2AX. Strongest responses were elicited by paraquat, profenofos, chlorpyrifos, cypermethrin, and the S1 mixture, which contained 25% paraquat. Comet assay revealed significant increases of DNA damage in response to paraquat, cypermethrin, and U2 and S1 mixtures, which contained high levels of cypermethrin and paraquat, respectively. In summary, we showed that the tested pesticides, alone or in mixtures, in general induced oxidative stress and that most pesticides, and especially paraquat and cypermethrin, were genotoxic in HepG2 cells. We could also show that mixtures dominated by these two pesticides displayed a marked genotoxic potency, which agreed with our previous population studies.

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Cytogenetic damage in peripheral blood lymphocytes of children exposed to pesticides in agricultural areas of the department of Cordoba, Colombia

Cited by 25 publications

References 33 publications

Toxicoproteomics Disclose Pesticides as Downregulators of TNF-α, IL-1β and Estrogen Receptor Pathways in Breast Cancer Women Chronically Exposed

Toxicoproteomics Disclose Pesticides as Downregulators of TNF-α, IL-1β and Estrogen Receptor Pathways in Breast Cancer Women Chronically Exposed

Potential Health Risks Linked to Emerging Contaminants in Major Rivers and Treated Waters

In vitro cytotoxicity and genotoxicity of single and combined pesticides used by Bolivian farmers

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