Assembly of β-glucosidase multilayers on spherical colloidal particles and their use as active catalysts

Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications

“…[46] Proteins that are electrostatically attracted to the charged nanoparticles can provide an interface for the further deposition of an oppositely charged polyelectrolyte polymer, which again allows the deposition of a secondary protein layer. Multilayer films of BSA, [47] IgG, [47] b-glucosidase, [48] glucose oxidase (GOx), [49,50] urease, [51] and horseradish peroxidase (HRP) [50] have been assembled on polystyrene NPs by the alternate deposition of the proteins and an oppositely charged synthetic polyelectrolyte as a linker (e.g. poly(diallyldimethylammonium chloride) or poly(sodium 4-styrenesulfonate) were used as positively or negatively charged polyelectrolytes, respectively).…”

“…Au nanoparticles (13 nm) that were functionalized with oligonucleotide 48 were then added to yield a monolayer of ds-DNA (ds-47/48) attached to the Au NPs (Figure 20 A). As many of the DNA chains (48) around the Au NP are not treated with the surface-confined DNA 47, they can be reacted with further complementary domains of oligonucleotide 47 to provide binding sites for 46. A second type of Au NP modified with the oligonucleotide 46 was treated with this first layer assembly to result in a second layer of Au NPs.…”

Tailor‐Made Polyelectrolyte Microcapsules: From Multilayers to Smart Containers

“…Biocolloid particles with tailored enzymatic activities can be produced by means of protein multilayer architectures on colloidal particles. Multilayer films of bovine serum albumine, [110] immunoglobulin alone [110] or in combination with a fluorescent label, [111] b-glucosidase, [112] glucose oxidase, [113][114][115][116] horseradish peroxidase, [113] and urease [117] have been assembled on polystyrene microspheres by the alternate deposition of the protein and oppositely charged polymer. The protein multilayer shell could be varied from several nanometers in thickness to hundreds of nanometers.…”

Maßgeschneiderte Polyelektrolyt‐Mikrokapseln: von Multischichten zu smarten Kapseln

“…[104] Die Calcinierung von Kern-Hülle-Strukturen mit magnetischen Nanopartikeln bei erhöhter Temperatur liefert ebenfalls intakte magnetische Kugeln. [103] Andere Kern-Hülle-Systeme, beispielsweise auf Titanbasis [106,107] oder auf der Basis lumineszierender CdTe-Halbleiterkristalle, [108,109] [110] unmarkiertem [110] oder fluoreszenzmarkiertem [111] Immunglobulin, b-Glucosidase, [112] Glucoseoxidase, [113][114][115][116] Meerrettich-Peroxidase [113] und Urease [117] [150] PAH/PSS-Kapseln konnten ohne Verlust der Kapselform und -stabilität durch schrittweisen Lösungsmittelaustausch in unterschiedlichen organischen Medien suspendiert werden, [122] wobei steigende Permeabilitäten in organischen Lösungsmitteln auf Veränderungen in der Wandstruktur schließen lassen. [123] beschrieben wurde, die Größe der entstehenden MF-Oligomere bestimmt, die durch die Kapselwand nach außen gelangen müssen.…”