Assembly, Function, and Dynamics of the Photosynthetic Machinery in Chlamydomonas reinhardtii

Rochaix, J.-D.

doi:10.1104/pp.127.4.1394

Cited by 16 publications

(20 citation statements)

References 12 publications

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“…The primary photochemistry leading to photosynthetic oxygen evolution and electron flow in oxygenic organisms involves a supramolecular protein-pigment complex, photosystem II (PSII) [1]–[3]. PSII is dominated by the D1–D2 reaction center protein heterodimer, which is sensitive to environmental stresses including damage from light and UV irradiation [2], [4].…”

Section: Introductionmentioning

confidence: 99%

Mutations of Photosystem II D1 Protein That Empower Efficient Phenotypes of Chlamydomonas reinhardtii under Extreme Environment in Space

et al. 2013

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Space missions have enabled testing how microorganisms, animals and plants respond to extra-terrestrial, complex and hazardous environment in space. Photosynthetic organisms are thought to be relatively more prone to microgravity, weak magnetic field and cosmic radiation because oxygenic photosynthesis is intimately associated with capture and conversion of light energy into chemical energy, a process that has adapted to relatively less complex and contained environment on Earth. To study the direct effect of the space environment on the fundamental process of photosynthesis, we sent into low Earth orbit space engineered and mutated strains of the unicellular green alga, Chlamydomonas reinhardtii, which has been widely used as a model of photosynthetic organisms. The algal mutants contained specific amino acid substitutions in the functionally important regions of the pivotal Photosystem II (PSII) reaction centre D1 protein near the QB binding pocket and in the environment surrounding Tyr-161 (YZ) electron acceptor of the oxygen-evolving complex. Using real-time measurements of PSII photochemistry, here we show that during the space flight while the control strain and two D1 mutants (A250L and V160A) were inefficient in carrying out PSII activity, two other D1 mutants, I163N and A251C, performed efficient photosynthesis, and actively re-grew upon return to Earth. Mimicking the neutron irradiation component of cosmic rays on Earth yielded similar results. Experiments with I163N and A251C D1 mutants performed on ground showed that they are better able to modulate PSII excitation pressure and have higher capacity to reoxidize the QA − state of the primary electron acceptor. These results highlight the contribution of D1 conformation in relation to photosynthesis and oxygen production in space.

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Section: Introductionmentioning

confidence: 99%

Mutations of Photosystem II D1 Protein That Empower Efficient Phenotypes of Chlamydomonas reinhardtii under Extreme Environment in Space

et al. 2013

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“…Several genes that do not encode structural components of PSI have been shown to be important for the stability or the assembly of PSI (Boudreau et al ., 1997; Chitnis, 2001; Moseley et al ., 2002; Rochaix, 2001; Wilde et al ., 2001; Zak and Pakrasi, 2000). Among those are HCF101 of Arabidopsis , which is involved in PSI accumulation (Meurer et al ., 1996a), and the cyanobacterial rubredoxin RubA, which appears to be involved in protection of the F x cluster during PSI assembly (Shen et al ., 2002).…”

Section: Introductionmentioning

confidence: 99%

The universally conserved HCF101 protein is involved in assembly of [4Fe‐4S]‐cluster‐containing complexes in Arabidopsis thaliana chloroplasts

Lezhneva

Amann

Meurer³

2003

The Plant Journal

126

104

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SummaryThe seedling-lethal nuclear Arabidopsis hcf101 (high chlorophyll¯uorescence) mutant is impaired in photosynthesis and complemented by the wild-type HCF101 cDNA. Photosystem I (PSI) activity is abolished, and PSI core complexes fail to accumulate in hcf101, whereas levels of other thylakoid membrane proteins are unaffected. Northern and in vivo labelling analyses as well as studies on polysome loading show that PSI transcript levels and translation rates of proteins, which belong to PSI, are normal in hcf101. PSI-speci®c uorescence at 77 K is shifted from 735 to 728 nm in hcf101, indicating that exitons cannot ef®ciently be transferred to the PSI reaction centre, whereby the PSI antenna is almost unaffected. Mutant plants not only fail to accumulate mature PSI, which contains three [4Fe-4S] clusters (FSCs), but also are characterized by reduced levels of the soluble FSC-containing complex ferredoxin-thioredoxin reductase (FTR) in the stroma. Inhibited FTR maturation is not a secondary effect stemming from lack of PSI because the mutant hcf145, which also lacks PSI, accumulates FTR at normal levels. Levels of the [2Fe-2S] clustercontaining soluble and membrane proteins, ferredoxin and PetC, respectively, were unchanged in hcf101 plants. These data suggest a speci®c role of HCF101 in FSC biogenesis. HCF101 is plastid localized and belongs to an ancient and universally conserved family of P-loop ATPases previously designated as thè MRP' (metG related protein) family. The function identi®ed for HCF101 suggests a new designation, FSC, for this family.

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“…There was a general consensus that the light-induced damage to the photosynthetic machinery was particularly serious in PSII (Ohad et al 1990;Rochaix 2001). The photoinhibition of photosynthesis was such a major stress that the rate of lightinduced inactivation of the reaction center exceeded the rate of the recovery process from the light-induced inactivated state.…”

Section: Mechanism and Differences Of Responses To Two Pdfs Between Jmentioning

confidence: 99%

Changes in Unsaturated Levels of Fatty Acids in Thylakoid PSII Membrane Lipids During Chilling‐induced Resistance in Rice

Zhu

et al. 2007

JIPB

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Temperature is one of the abiotic factors limiting growth and productivity of plants. In the present work, the effect of low non-freezing temperature, as an inducer of "chilling resistance", was studied in three cultivars of rice (Oryza sativa L.), japonica cv. 9516 (j-9516), the two parental lines of superhigh-yield hybrid rice between subspecies, Peiai/E32 (ji-PE), and the traditional indica hybrid rice Shanyou 63 (i-SY63). Leaves of chill-treated rice showed chilling-induced resistance, as an increase of their low-temperature tolerance was measured using chlorophyll fluorescence measurements, revealing a change in photosystem II (PSII) efficiency.

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Assembly, Function, and Dynamics of the Photosynthetic Machinery in Chlamydomonas reinhardtii

Cited by 16 publications

References 12 publications

Mutations of Photosystem II D1 Protein That Empower Efficient Phenotypes of Chlamydomonas reinhardtii under Extreme Environment in Space

Mutations of Photosystem II D1 Protein That Empower Efficient Phenotypes of Chlamydomonas reinhardtii under Extreme Environment in Space

The universally conserved HCF101 protein is involved in assembly of [4Fe‐4S]‐cluster‐containing complexes in Arabidopsis thaliana chloroplasts

Changes in Unsaturated Levels of Fatty Acids in Thylakoid PSII Membrane Lipids During Chilling‐induced Resistance in Rice

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