Plants have evolved a variety of mechanisms to adapt to N starvation. NITRATE TRANSPORTER2.4 (NRT2.4) is one of seven NRT2 family genes in Arabidopsis thaliana, and NRT2.4 expression is induced under N starvation. Green fluorescent protein and b-glucuronidase reporter analyses revealed that NRT2.4 is a plasma membrane transporter expressed in the epidermis of lateral roots and in or close to the shoot phloem. The spatiotemporal expression pattern of NRT2.4 in roots is complementary with that of the major high-affinity nitrate transporter NTR2.1. Functional analysis in Xenopus laevis oocytes and in planta showed that NRT2.4 is a nitrate transporter functioning in the high-affinity range. In N-starved nrt2.4 mutants, nitrate uptake under low external supply and nitrate content in shoot phloem exudates was decreased. In the absence of NRT2.1 and NRT2.2, loss of function of NRT2.4 (triple mutants) has an impact on biomass production under low nitrate supply. Together, our results demonstrate that NRT2.4 is a nitrate transporter that has a role in both roots and shoots under N starvation. INTRODUCTIONNitrate (NO 3 -) uptake from the soil and distribution through the plant can profoundly affect plant growth and productivity. Nitrogen (N) limitation decreases crop yield worldwide. To meet expanding food demands, the global use of N fertilizer in agricultural production is projected to increase threefold to reach 249 million tons annually by the year 2050 (Tilman et al., 2001). However, the recovery of N fertilizer by crops is low, with in some cases only 30 to 50% of the applied N being taken up by the crop (Peoples et al., 1995;Sylvester-Bradley and Kindred, 2009). The remainder is partly used by subsequent crops but can also be lost from the agro-ecosystem, and fertilizer runoff into aquatic systems leads to environmentally harmful eutrophication (Tilman, 1998). Therefore, improving N uptake efficiency is important to reduce the costs of crop production and pollution damage. Beside N uptake, N remobilization is another key step to improve N use efficiency in crops (Mickelson et al., 2003;Masclaux-Daubresse et al., 2008).Plants have evolved versatile mechanisms to cope with N limitation and N starvation, and besides major adaptive changes of the root system architecture (Drew and Saker, 1975), root NO 3 -uptake characteristics are regulated in response to N availability (Clarkson et al., 1986;Lejay et al., 1999;Glass, 2003). Physiological studies have led to the conclusion that at least three NO 3 -uptake systems are responsible for the influx of NO 3 -into roots (reviewed in Crawford and Glass, 1998;Daniel-Vedele et al., 1998;Forde, 2000). Two high-affinity transport systems (HATS) operate to take up NO 3 -at low concentrations in the external medium, and both display saturable kinetics as a function of the external NO 3 -concentration, with saturation in the range of 0.2 to 0.5 mM. The first one, constitutive HATS, is active in plants that have not been supplied with NO 3 -, whereas the second HATS is induced by NO ...
SUMMARYNitrogen is a key mineral nutrient playing a crucial role in plant growth and development. Understanding the mechanisms of nitrate uptake from the soil and distribution through the plant in response to nitrogen starvation is an important step on the way to improve nitrogen uptake and utilization efficiency for better growth and productivity of plants, and to prevent negative effects of nitrogen fertilizers on the environment and human health. In this study, we show that Arabidopsis NITRATE TRANSPORTER 2.5 (NRT2.5) is a plasma membrane-localized high-affinity nitrate transporter playing an essential role in adult plants under severe nitrogen starvation. NRT2.5 expression is induced under nitrogen starvation and NRT2.5 becomes the most abundant transcript amongst the seven NRT2 family members in shoots and roots of adult plants after long-term starvation. GUS reporter analyses showed that NRT2.5 is expressed in the epidermis and the cortex of roots at the root hair zone and in minor veins of mature leaves. Reduction of NRT2.5 expression resulted in a decrease in high-affinity nitrate uptake without impacting low-affinity uptake. In the background of the high-affinity nitrate transporter mutant nrt2.4, an nrt2.5 mutation reduced nitrate levels in the phloem of N-starved plants further than in the single nrt2.4 mutants. Growth analyses of multiple mutants between NRT2.1, NRT2.2, NRT2.4, and NRT2.5 revealed that NRT2.5 is required to support growth of nitrogen-starved adult plants by ensuring the efficient uptake of nitrate collectively with NRT2.1, NRT2.2 and NRT2.4 and by taking part in nitrate loading into the phloem during nitrate remobilization.
Phylloquinone is a compound present in all photosynthetic plants serving as cofactor for Photosystem I-mediated electron transport. Newly identified seedling-lethal Arabidopsis thaliana mutants impaired in the biosynthesis of phylloquinone possess reduced Photosystem I activity. The affected gene, called PHYLLO, consists of a fusion of four previously individual eubacterial genes, menF, menD, menC, and menH, required for the biosynthesis of phylloquinone in photosynthetic cyanobacteria and the respiratory menaquinone in eubacteria. The fact that homologous men genes reside as polycistronic units in eubacterial chromosomes and in plastomes of red algae strongly suggests that PHYLLO derived from a plastid operon during endosymbiosis. The principle architecture of the fused PHYLLO locus is conserved in the nuclear genomes of plants, green algae, and the diatom alga Thalassiosira pseudonana. The latter arose from secondary endosymbiosis of a red algae and a eukaryotic host indicating selective driving forces for maintenance and/or independent generation of the composite gene cluster within the nuclear genomes. Besides, individual menF genes, encoding active isochorismate synthases (ICS), have been established followed by splitting of the essential 3 region of the menF module of PHYLLO only in genomes of higher plants. This resulted in inactivation of the ICS activity encoded by PHYLLO and enabled a metabolic branch from the phylloquinone biosynthetic route to independently regulate the synthesis of salicylic acid required for plant defense. Therefore, gene fusion, duplication, and fission events adapted a eubacterial multienzymatic system to the metabolic requirements of plants.
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