Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix

Du, Mingchun; Zhu, Y.; Yuan, Lan; Liang, Hui; Mou, Chenchen; Li, Xiaoran; Zhuang, Yan; Wen, Zhang; Shi, Qin; Chen, Bing; Dai, Jianwu

doi:10.1016/j.colsurfa.2013.05.044

Cited by 17 publications

(9 citation statements)

References 54 publications

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“…Ascorbic acid has large impact on tissues regeneration due to improving collagen synthesis [20], which is a component of the primary extracellular matrix (ECM) [21], which supports cells attachment, growth, and proliferation [22]. Some literature data show that researchers took an attempt to incorporate AA into the polyurethane structure to improve the material biocompatibility and biodegradability [23,24].…”

Section: Introductionmentioning

confidence: 99%

Thermal and mechanical properties of polyurethanes modified with L-ascorbic acid

Kucińska-Lipka

Gubańska

Sienkiewicz

2016

J Therm Anal Calorim

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In this study we report the thermal and mechanical properties of polyurethanes modified with ascorbic acid (AA). Ascorbic acid was used as a modifier at concentration of 1 or 2 mass%. The antioxidative properties of AA may improve the biocompatibility of the obtained materials, which were designed for biomedical applications. In this paper we describe characterization of obtained unmodified and ascorbic acid modified polyurethanes with the use of following methods: dynamic mechanical analysis, thermogravimetric analysis and mechanical tests including tensile strength, elongation at break, abrasive resistance, and hardness. Results of performed studies suggests that synthesized polyurethane materials may be suitable candidates for biomedical applications such as tissue scaffolds or implants, where required tensile strength is in the range of 1-14 MPa and elongation at break is approximately in the range of 100-380 %.

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Section: Introductionmentioning

confidence: 99%

Thermal and mechanical properties of polyurethanes modified with L-ascorbic acid

Kucińska-Lipka

Gubańska

Sienkiewicz

2016

J Therm Anal Calorim

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“…Recent works have produced a wealth of data on the advantages offered by new physical forms of chitosan stabilized with genipin [ 95 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 ]. The advances made become quite noticeable when the works dated 2010–2015 (amounting to >82% of the bibliography below) are compared to some most significant key articles [ 5 , 11 , 27 , 41 , 50 , 141 , 142 , 143 , 144 , 145 , 146 ] published in the 2000–2005 quinquennium.…”

Section: Discussionmentioning

confidence: 99%

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

et al. 2015

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The present review article intends to direct attention to the technological advances made since 2009 in the area of genipin-crosslinked chitosan (GEN-chitosan) hydrogels. After a concise introduction on the well recognized characteristics of medical grade chitosan and food grade genipin, the properties of GEN-chitosan obtained with a safe, spontaneous and irreversible chemical reaction, and the quality assessment of the gels are reviewed. The antibacterial activity of GEN-chitosan has been well assessed in the treatment of gastric infections supported by Helicobacter pylori. Therapies based on chitosan alginate crosslinked with genipin include stem cell transplantation, and development of contraction free biomaterials suitable for cartilage engineering. Collagen, gelatin and other proteins have been associated to said hydrogels in view of the regeneration of the cartilage. Viability and proliferation of fibroblasts were impressively enhanced upon addition of poly-l-lysine. The modulation of the osteocytes has been achieved in various ways by applying advanced technologies such as 3D-plotting and electrospinning of biomimetic scaffolds, with optional addition of nano hydroxyapatite to the formulations. A wealth of biotechnological advances and know-how has permitted reaching outstanding results in crucial areas such as cranio-facial surgery, orthopedics and dentistry. It is mandatory to use scaffolds fully characterized in terms of porosity, pore size, swelling, wettability, compressive strength, and degree of acetylation, if the osteogenic differentiation of human mesenchymal stem cells is sought: in fact, the novel characteristics imparted by GEN-chitosan must be simultaneously of physico-chemical and cytological nature. Owing to their high standard, the scientific publications dated 2010–2015 have met the expectations of an interdisciplinary audience.

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“…Quantification of total glycosaminoglycans (GAG) in chondrocyte cultures is necessary for the complete assessment of the metabolic profile of the system and is commonly performed using the 1,9-dimethylmethylene blue (DMB) dye method [22,23]. The artificial cells obtained after determinate time were cultured with 300 μL papain in a water-bath at 60 °C overnight and then were centrifuged at 4000 rpm for 5 min to obtain supernatant samples.…”

Section: Methodsmentioning

confidence: 99%

The Influence of bFGF on the Fabrication of Microencapsulated Cartilage Cells under Different Shaking Modes

et al. 2019

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Cell encapsulation in hydrogels has been extensively used in cytotherapy, regenerative medicine, 3D cell culture, and tissue engineering. Herein, we fabricated microencapsulated cells through microcapsules loaded with C5.18 chondrocytes alginate/chitosan prepared by a high-voltage electrostatic method. Under optimized conditions, microencapsulated cells presented uniform size distribution, good sphericity, and a smooth surface with different cell densities. The particle size distribution was determined at 150–280 μm, with an average particle diameter of 220 μm. The microencapsulated cells were cultured under static, shaking, and 3D micro-gravity conditions with or without bFGF (basic fibroblast growth factor) treatment. The quantified detection (cell proliferation detection and glycosaminoglycan (GAG)/type II collagen (Col-II)) content was respectively determined by cell counting kit-8 assay (CCK-8) and dimethylmethylene blue (DMB)/Col-II secretion determination) and qualitative detection (acridine orange/ethidium bromide, hematoxylin-eosin, alcian blue, safranin-O, and immunohistochemistry staining) of these microencapsulated cells were evaluated. Results showed that microencapsulated C5.18 cells under three-dimensional microgravity conditions promoted cells to form large cell aggregates within 20 days by using bFGF, which provided the possibility for cartilage tissue constructs in vitro. It could be found from the cell viability (cell proliferation) and synthesis (content of GAG and Col-II) results that microencapsulated cells had a better cell proliferation under 3D micro-gravity conditions using bFGF than under 2D conditions (including static and shaking conditions). We anticipate that these results will be a benefit for the design and construction of cartilage regeneration in future tissue engineering applications.

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Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix

Cited by 17 publications

References 54 publications

Thermal and mechanical properties of polyurethanes modified with L-ascorbic acid

Thermal and mechanical properties of polyurethanes modified with L-ascorbic acid

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

The Influence of bFGF on the Fabrication of Microencapsulated Cartilage Cells under Different Shaking Modes

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